دورية أكاديمية
أعرض في EDS

Identification of novel, functional, long noncoding RNAs involved in programmed, large-scale genome rearrangements.

التفاصيل البيبلوغرافية
العنوان: Identification of novel, functional, long noncoding RNAs involved in programmed, large-scale genome rearrangements.
المؤلفون: Bechara ST; Institute of Cell Biology, University of Bern, Bern 3012, Switzerland.; Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern 3012, Switzerland., Kabbani LES; Institute of Cell Biology, University of Bern, Bern 3012, Switzerland.; Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern 3012, Switzerland., Maurer-Alcalá XX; Institute of Cell Biology, University of Bern, Bern 3012, Switzerland.; Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern 3012, Switzerland., Nowacki M; Institute of Cell Biology, University of Bern, Bern 3012, Switzerland.
المصدر: RNA (New York, N.Y.) [RNA] 2022 Aug; Vol. 28 (8), pp. 1110-1127. Date of Electronic Publication: 2022 Jun 09.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Cold Spring Harbor Laboratory Press Country of Publication: United States NLM ID: 9509184 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1469-9001 (Electronic) Linking ISSN: 13558382 NLM ISO Abbreviation: RNA Subsets: MEDLINE
أسماء مطبوعة: Publication: <2003->: Cold Spring Harbor, NY : Cold Spring Harbor Laboratory Press
Original Publication: New York, NY : Cambridge University Press, c1995-
مواضيع طبية MeSH: Paramecium tetraurelia*/genetics , RNA, Long Noncoding*/genetics, Cell Nucleus/genetics ; DNA Transposable Elements/genetics ; Gene Rearrangement ; RNA, Untranslated/genetics
مستخلص: Noncoding RNAs (ncRNAs) make up to ∼98% percent of the transcriptome of a given organism. In recent years, one relatively new class of ncRNAs, long noncoding RNAs (lncRNAs), were shown to be more than mere by-products of gene expression and regulation. The unicellular eukaryote Paramecium tetraurelia is a member of the ciliate phylum, an extremely heterogeneous group of organisms found in most bodies of water across the globe. A hallmark of ciliate genetics is nuclear dimorphism and programmed elimination of transposons and transposon-derived DNA elements, the latter of which is essential for the maintenance of the somatic genome. Paramecium and ciliates in general harbor a plethora of different ncRNA species, some of which drive the process of large-scale genome rearrangements, including DNA elimination, during sexual development. Here, we identify and validate the first known functional lncRNAs in ciliates to date. Using deep-sequencing and subsequent bioinformatic processing and experimental validation, we show that Paramecium expresses at least 15 lncRNAs. These candidates were predicted by a highly conservative pipeline, and informatic analyses hint at differential expression during development. Depletion of two lncRNAs, lnc1 and lnc15, resulted in clear phenotypes, decreased survival, morphological impairment, and a global effect on DNA elimination.
(© 2022 Bechara et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.)
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فهرسة مساهمة: Keywords: DNA elimination; ciliate; genome rearrangement; lncRNA; sRNA
المشرفين على المادة: 0 (DNA Transposable Elements)
0 (RNA, Long Noncoding)
0 (RNA, Untranslated)
تواريخ الأحداث: Date Created: 20220609 Date Completed: 20220720 Latest Revision: 20220830
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC9297840
DOI: 10.1261/rna.079134.122
PMID: 35680167
قاعدة البيانات: MEDLINE
الوصف
تدمد:1469-9001
DOI:10.1261/rna.079134.122