دورية أكاديمية
7-epi-cylindrospermopsin and microcystin producers among diverse Anabaena/Dolichospermum/Aphanizomenon CyanoHABs in Oregon, USA.
| العنوان: | 7-epi-cylindrospermopsin and microcystin producers among diverse Anabaena/Dolichospermum/Aphanizomenon CyanoHABs in Oregon, USA. |
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| المؤلفون: | Dreher TW; Department of Microbiology, Oregon State University, Corvallis, OR 97331, USA. Electronic address: theo.dreher@oregonstate.edu., Foss AJ; GreenWater Laboratories, 205 Zeagler Drive, Suite 302, Palatka, FL 32177, USA. Electronic address: amandafoss@greenwaterlab.com., Davis EW 2nd; Center for Quantitative Life Sciences, Oregon State University, Corvallis, OR 97331, USA., Mueller RS; Department of Microbiology, Oregon State University, Corvallis, OR 97331, USA. |
| المصدر: | Harmful algae [Harmful Algae] 2022 Jul; Vol. 116, pp. 102241. Date of Electronic Publication: 2022 May 16. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101128968 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-1470 (Electronic) Linking ISSN: 15689883 NLM ISO Abbreviation: Harmful Algae Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam ; New York : Elsevier, c2002- |
| مواضيع طبية MeSH: | Anabaena* , Aphanizomenon*/genetics , Bacterial Toxins* , Cyanobacteria*/genetics, Alkaloids ; Chromatography, Liquid ; Cyanobacteria Toxins ; Microcystins ; Oregon ; Tandem Mass Spectrometry |
| مستخلص: | Several genomes of Nostocales ADA clade members from the US Pacific Northwest were recently sequenced. Biosynthetic genes for microcystin, cylindrospermopsin or anatoxin-a were present in 7 of the 15 Dolichospermum/Anabaena strains and none of the 5 Aphanizomenon flos-aquae (AFA) strains. Toxin analyses (ELISA and LC-MS/MS) were conducted to quantitate and identify microcystin (MC) and cylindrospermopsin (CYN) congeners/analogs in samples dominated by Dolichospermum spp. of known genome sequence. MC-LR was the main congener produced by Dolichospermum spp. from Junipers Reservoir, Lake Billy Chinook and Odell Lake, while a congener provisionally identified as [Dha 7 ]MC-HtyR was produced by a Dolichospermum sp. in Detroit Reservoir. A second Dolichospermum sp. from Detroit Reservoir was found to produce 7-epi-CYN, with 7-deoxy-CYN also present, but no CYN. The monitoring history of each of these lakes indicates the capacity for high levels of cyanotoxins during periods when Dolichospermum spp. are the dominant cyanobacteria. The diversity of ADA strains found in the US Pacific NW emphasizes the importance of these cyanobacteria as potentially toxic HAB formers in this temperate climatic region. Our results linking congener and genetic identity add data points that will help guide development of improved tools for predicting congener specificity from cyanotoxin gene sequences. (Copyright © 2022 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: 7-epi-cylindrospermopsin; CyanoHAB; Microcystin, homotyrosine; NRPS, specificity prediction; mcy genes |
| المشرفين على المادة: | 0 (Alkaloids) 0 (Bacterial Toxins) 0 (Cyanobacteria Toxins) 0 (Microcystins) 2JIZ556BA3 (cylindrospermopsin) |
| تواريخ الأحداث: | Date Created: 20220616 Date Completed: 20220620 Latest Revision: 20220827 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.hal.2022.102241 |
| PMID: | 35710201 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1878-1470 |
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| DOI: | 10.1016/j.hal.2022.102241 |