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A Malaria Parasite Cross Reveals Genetic Determinants of Plasmodium falciparum Growth in Different Culture Media.

التفاصيل البيبلوغرافية
العنوان: A Malaria Parasite Cross Reveals Genetic Determinants of Plasmodium falciparum Growth in Different Culture Media.
المؤلفون: Kumar S; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA, United States., Li X; Program in Disease Intervention and Prevention, Texas Biomedical Research Institute, San Antonio, TX, United States., McDew-White M; Program in Disease Intervention and Prevention, Texas Biomedical Research Institute, San Antonio, TX, United States., Reyes A; Program in Disease Intervention and Prevention, Texas Biomedical Research Institute, San Antonio, TX, United States., Delgado E; Program in Disease Intervention and Prevention, Texas Biomedical Research Institute, San Antonio, TX, United States., Sayeed A; Program in Disease Intervention and Prevention, Texas Biomedical Research Institute, San Antonio, TX, United States., Haile MT; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA, United States., Abatiyow BA; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA, United States., Kennedy SY; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA, United States., Camargo N; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA, United States., Checkley LA; Department of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN, United States., Brenneman KV; Department of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN, United States., Button-Simons KA; Department of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN, United States., Duraisingh MT; Immunology and Infectious Diseases Department, Harvard T.H. Chan School of Public Health, Boston, MA, United States., Cheeseman IH; Program in Host Pathogen Interactions, Texas Biomedical Research Institute, San Antonio, TX, United States., Kappe SHI; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA, United States.; Department of Pediatrics, University of Washington, Seattle, WA, United States., Nosten F; Shoklo Malaria Research Unit, Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Mae Sot, Thailand.; Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine Research building, University of Oxford, Oxford, United Kingdom., Ferdig MT; Department of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN, United States., Vaughan AM; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA, United States.; Department of Pediatrics, University of Washington, Seattle, WA, United States., Anderson TJC; Program in Disease Intervention and Prevention, Texas Biomedical Research Institute, San Antonio, TX, United States.
المصدر: Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2022 May 30; Vol. 12, pp. 878496. Date of Electronic Publication: 2022 May 30 (Print Publication: 2022).
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Frontiers Media SA Country of Publication: Switzerland NLM ID: 101585359 Publication Model: eCollection Cited Medium: Internet ISSN: 2235-2988 (Electronic) Linking ISSN: 22352988 NLM ISO Abbreviation: Front Cell Infect Microbiol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Lausanne : Frontiers Media SA
مواضيع طبية MeSH: Malaria, Falciparum*/parasitology , Plasmodium falciparum*/genetics , Plasmodium falciparum*/growth & development, Animals ; Crosses, Genetic ; Culture Media ; Gene Frequency ; Mice ; Quantitative Trait Loci
مستخلص: What genes determine in vitro growth and nutrient utilization in asexual blood-stage malaria parasites? Competition experiments between NF54, clone 3D7, a lab-adapted African parasite, and a recently isolated Asian parasite (NHP4026) reveal contrasting outcomes in different media: 3D7 outcompetes NHP4026 in media containing human serum, while NHP4026 outcompetes 3D7 in media containing AlbuMAX, a commercial lipid-rich bovine serum formulation. To determine the basis for this polymorphism, we conducted parasite genetic crosses using humanized mice and compared genome-wide allele frequency changes in three independent progeny populations cultured in media containing human serum or AlbuMAX. This bulk segregant analysis detected three quantitative trait loci (QTL) regions [on chromosome (chr) 2 containing aspartate transaminase AST ; chr 13 containing EBA-140; and chr 14 containing cysteine protease ATG4 ] linked with differential growth in serum or AlbuMAX in each of the three independent progeny pools. Selection driving differential growth was strong ( s = 0.10 - 0.23 per 48-hour lifecycle). We conducted validation experiments for the strongest QTL on chr 13: competition experiments between ΔEBA-140 and 3D7 wildtype parasites showed fitness reversals in the two medium types as seen in the parental parasites, validating this locus as the causative gene. These results (i) demonstrate the effectiveness of bulk segregant analysis for dissecting fitness traits in P. falciparum genetic crosses, and (ii) reveal intimate links between red blood cell invasion and nutrient composition of growth media. Use of parasite crosses combined with bulk segregant analysis will allow systematic dissection of key nutrient acquisition/metabolism and red blood cell invasion pathways in P. falciparum.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2022 Kumar, Li, McDew-White, Reyes, Delgado, Sayeed, Haile, Abatiyow, Kennedy, Camargo, Checkley, Brenneman, Button-Simons, Duraisingh, Cheeseman, Kappe, Nosten, Ferdig, Vaughan and Anderson.)
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معلومات مُعتمدة: P01 AI127338 United States AI NIAID NIH HHS; R37 AI048071 United States AI NIAID NIH HHS; R21 AI133369 United States AI NIAID NIH HHS
فهرسة مساهمة: Keywords: AlbuMAX; Plasmodium falciparum; bulk segregant analysis; genetic cross; serum
المشرفين على المادة: 0 (Culture Media)
تواريخ الأحداث: Date Created: 20220617 Date Completed: 20220620 Latest Revision: 20230516
رمز التحديث: 20230516
مُعرف محوري في PubMed: PMC9197316
DOI: 10.3389/fcimb.2022.878496
PMID: 35711667
قاعدة البيانات: MEDLINE
الوصف
تدمد:2235-2988
DOI:10.3389/fcimb.2022.878496