دورية أكاديمية
Hydroalcoholic extract of Remijia ferruginea accelerates the closure of skin wounds by modulating tissue morphology and antioxidant profile: An in vitro and in vivo study.
| العنوان: | Hydroalcoholic extract of Remijia ferruginea accelerates the closure of skin wounds by modulating tissue morphology and antioxidant profile: An in vitro and in vivo study. |
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| المؤلفون: | Sarandy MM; Department of General Biology, Federal University of Viçosa, Viçosa, Minas Gerais, 36570-900, Brazil. Electronic address: mariaureasarandy@gmail.com., Gusmão LJ; Department of Biochemistry and Molecular Biology, Federal University of Viçosa, Viçosa, Minas Gerais, 36570-900, Brazil. Electronic address: leandrojgusmao@gmail.com., Purgato GA; Department of Biochemistry and Molecular Biology, Federal University of Viçosa, Viçosa, Minas Gerais, 36570-900, Brazil. Electronic address: gislaine.aparecida@ufv.br., Píccolo MS; Department of Biochemistry and Molecular Biology, Federal University of Viçosa, Viçosa, Minas Gerais, 36570-900, Brazil. Electronic address: mayraspiccolo@gmail.com., Pinto da Matta SL; Department of General Biology, Federal University of Viçosa, Viçosa, Minas Gerais, 36570-900, Brazil. Electronic address: smatta@ufv.br., Pizziolo VR; Department of Biochemistry and Molecular Biology, Federal University of Viçosa, Viçosa, Minas Gerais, 36570-900, Brazil. Electronic address: virginia.pizziolo@ufv.br., Diaz-Munoz G; Department of Chemistry, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, 31270-901, Brazil. Electronic address: gaspardm@qui.ufmg.br., Nogueira Diaz MA; Department of Biochemistry and Molecular Biology, Federal University of Viçosa, Viçosa, Minas Gerais, 36570-900, Brazil. Electronic address: marisanogueira@ufv.br. |
| المصدر: | Journal of ethnopharmacology [J Ethnopharmacol] 2022 Oct 05; Vol. 296, pp. 115464. Date of Electronic Publication: 2022 Jun 15. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Sequoia Country of Publication: Ireland NLM ID: 7903310 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7573 (Electronic) Linking ISSN: 03788741 NLM ISO Abbreviation: J Ethnopharmacol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Limerick : Elsevier Sequoia Original Publication: Lausanne, Elsevier Sequoia. |
| مواضيع طبية MeSH: | Antioxidants*/therapeutic use , Rubiaceae*, Animals ; Ointments/pharmacology ; Plant Extracts/therapeutic use ; Plant Leaves/chemistry ; Rats ; Rats, Wistar |
| مستخلص: | Ethnopharmacological Relevance: Remijia ferruginea DC. (Rubiaceae) (syn. Cinchona ferruginea A.St.-Hil.) is used in traditional medicine for the treatment of wounds, fever and malaria. Aim: This study investigated in vitro the proliferative and antioxidant effects of hydroalcoholic extract of leaves of R. ferruginea (HERF) and in vivo the healing effect of ointment based on HERF. Materials and Methods: The plant extract was characterized by liquid chromatography/mass spectrometry. Cell proliferation assays and in vitro antioxidant activity were performed. In in vivo assays, wound contraction ax was evaluated, as well as histological analyzes such as cellularity, proportion of blood vessels and collagen type I and III index. In addition, analyzes of the antioxidant enzymes SOD, CAT and GST were performed. Results: Our results showed in the chromatographic analysis that catechin, rutin and quercetin were the main phenolic compounds in the plant extract and may be responsible for the antioxidant and proliferative effects (p < 0.05). In addition, these compounds were found in higher concentration in leaves collected in spring. The ointment containing HERF was able to modulate tissue morphology, increasing cell proliferation, blood vessels, being able to stimulate the production of collagen fibers type I and III, (p < 0.05) contributing to scar tissue maturation and resistance. Conclusion: Our findings indicated that the three doses of HERF tested (1%, 3% and 5%) can modulate the skin repair process, but the best effects were observed after exposure to the highest dose. (Copyright © 2022 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Antioxidants defenses; Cells proliferation; Flavonoids; Herbal medicine; Wound heling |
| المشرفين على المادة: | 0 (Antioxidants) 0 (Ointments) 0 (Plant Extracts) |
| تواريخ الأحداث: | Date Created: 20220617 Date Completed: 20220719 Latest Revision: 20220719 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.jep.2022.115464 |
| PMID: | 35714880 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1872-7573 |
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| DOI: | 10.1016/j.jep.2022.115464 |