دورية أكاديمية
أعرض في EDS

Tumor necrosis factor signaling during equine placental infection leads to pro-apoptotic and necroptotic outcomes.

التفاصيل البيبلوغرافية
العنوان: Tumor necrosis factor signaling during equine placental infection leads to pro-apoptotic and necroptotic outcomes.
المؤلفون: Fedorka CE; Department of Veterinary Science, University of Kentucky, Lexington, KY, USA. Electronic address: Carleigh.Fedorka@uky.edu., El-Sheikh Ali H; Department of Veterinary Science, University of Kentucky, Lexington, KY, USA; Department of Theriogenology, Mansoura University, Dakahlia, Eqypt., Scoggin KE; Department of Veterinary Science, University of Kentucky, Lexington, KY, USA., Loux SC; Department of Veterinary Science, University of Kentucky, Lexington, KY, USA., Ball BA; Department of Veterinary Science, University of Kentucky, Lexington, KY, USA., Troedsson MHT; Department of Veterinary Science, University of Kentucky, Lexington, KY, USA.
المصدر: Journal of reproductive immunology [J Reprod Immunol] 2022 Aug; Vol. 152, pp. 103655. Date of Electronic Publication: 2022 Jun 11.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Ireland NLM ID: 8001906 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7603 (Electronic) Linking ISSN: 01650378 NLM ISO Abbreviation: J Reprod Immunol Subsets: MEDLINE
أسماء مطبوعة: Publication: Limerick : Elsevier/North-Holland Biomedical Press
Original Publication: Amsterdam, Elsevier/North-Holland Biomedical Press.
مواضيع طبية MeSH: Chorioamnionitis*/pathology , Horse Diseases*/metabolism , Horse Diseases*/pathology , Placenta Diseases* , Streptococcus equi*, Animals ; Cytokines ; Female ; Horses ; Humans ; Placenta/pathology ; Pregnancy ; Tumor Necrosis Factor-alpha ; Tumor Necrosis Factors
مستخلص: Ascending placentitis is the leading cause of abortion in the horse. The pleiotropic cytokine tumor necrosis factor (TNF) is an upstream regulator of this disease, but little is understood regarding its function in pregnancy maintenance or placental infection. To assess this, RNA sequencing was performed on chorioallantois and endometrium of healthy pregnant mares at various gestational lengths (n = 4/gestational age), in addition to postpartum chorioallantois, and diestrus endometrium to assess expression of TNF, TNFR-1, and TNFR-2. Additionally, ascending placentitis was induced via trans-cervical inoculation of S. equi spp. zooepidemicus in pregnant mares (n = 6 infected / n = 6 control) and tissues and serum were collected to evaluate TNF-related transcripts. IHC was performed to confirm protein localization of TNFR-1 and TNFR-2. In healthy pregnancy, TNFR-1 appears to be the predominant TNF-related receptor. Following induction of disease, TNF concentrations increased in maternal serum, but expression did not alter at the tissue level. While both TNFR-1 and TNFR-2 increased following induction of disease, alterations in downstream pathways indicate that TNFR-1 is the dominant receptor in ascending placentitis, and is primarily activated within the chorioallantois, with minimal signaling occurring within the endometrium. In conclusion, TNF appears to be involved in the pathophysiology of ascending placentitis. An increase in this cytokine during disease progression is believed to activate TNFR-1 within the chorioallantois, leading to various pro-apoptotic and necroptotic outcomes, all of which may signal for fetal demise and impending abortion.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Chorioallantois; Cytokine; Infectious abortion; Placentitis; Tumor necrosis factor
المشرفين على المادة: 0 (Cytokines)
0 (Tumor Necrosis Factor-alpha)
0 (Tumor Necrosis Factors)
تواريخ الأحداث: Date Created: 20220618 Date Completed: 20220719 Latest Revision: 20220927
رمز التحديث: 20231215
DOI: 10.1016/j.jri.2022.103655
PMID: 35716439
قاعدة البيانات: MEDLINE
الوصف
تدمد:1872-7603
DOI:10.1016/j.jri.2022.103655