دورية أكاديمية
أعرض في EDS

High genetic diversity of alphacoronaviruses in bat species (Mammalia: Chiroptera) from the Atlantic Forest in Brazil.

التفاصيل البيبلوغرافية
العنوان: High genetic diversity of alphacoronaviruses in bat species (Mammalia: Chiroptera) from the Atlantic Forest in Brazil.
المؤلفون: Bueno LM; Department of Veterinary Medicine, FZEA-USP, University of Sao Paulo, Pirassununga, Brazil., Rizotto LS; Graduate Program in Experimental Epidemiology Applied to Zoonoses, FMVZ-USP, University of São Paulo, São Paulo, Brazil., Viana AO; Institute of Biomedical Science, University of São Paulo, São Paulo, Brazil., Silva LMN; Graduate Program in Experimental Epidemiology Applied to Zoonoses, FMVZ-USP, University of São Paulo, São Paulo, Brazil., de Moraes MVDS; Graduate Program in Experimental Epidemiology Applied to Zoonoses, FMVZ-USP, University of São Paulo, São Paulo, Brazil., Benassi JC; Department of Veterinary Medicine, FZEA-USP, University of Sao Paulo, Pirassununga, Brazil., Scagion GP; Institute of Biomedical Science, University of São Paulo, São Paulo, Brazil., Dorlass EG; Institute of Biomedical Science, University of São Paulo, São Paulo, Brazil., Lopes BLT; Institute of Biomedical Science, University of São Paulo, São Paulo, Brazil., Cunha IN; Biological Museum, Instituto Butantan, São Paulo, Brazil., Melinski R; Biological Museum, Instituto Butantan, São Paulo, Brazil., de Alvarenga IF; Biological Museum, Instituto Butantan, São Paulo, Brazil., Leitão GL; Biological Museum, Instituto Butantan, São Paulo, Brazil., Rodrigues RC; Department of Biology, Federal Rural University of Pernambuco, Recife, Brazil., Pereira IMDS; Department of Biology, Federal Rural University of Pernambuco, Recife, Brazil., Dos Santos LDN; Department of Biology, Federal Rural University of Pernambuco, Recife, Brazil., Fisch F; School of Sea, Science and Technology, University of Vale do Itajaí, Itajaí, Brazil., Rocha AD; School of Sea, Science and Technology, University of Vale do Itajaí, Itajaí, Brazil., Port D; Brusque Educational Foundation, Brusque, Brazil., Pereira GS; School of Sea, Science and Technology, University of Vale do Itajaí, Itajaí, Brazil., Greatti A; Department of Genetics, Evolution and Bioagents, Institute of Biology, University of Campinas, Campinas, Brazil., Barnabé ACS; Department of Genetics, Evolution and Bioagents, Institute of Biology, University of Campinas, Campinas, Brazil., Tsukamoto J; Department of Genetics, Evolution and Bioagents, Institute of Biology, University of Campinas, Campinas, Brazil., Hingst-Zaher E; Biological Museum, Instituto Butantan, São Paulo, Brazil., de Azevedo SM Jr; Department of Biology, Federal Rural University of Pernambuco, Recife, Brazil., Telino WR Jr; Zoological Studies Laboratory, Federal University of Pernambuco Agreste, Garanhuns, Brazil., Branco JO; School of Sea, Science and Technology, University of Vale do Itajaí, Itajaí, Brazil., Ometto T; Institute of Biomedical Science, University of São Paulo, São Paulo, Brazil., de Araujo J; Institute of Biomedical Science, University of São Paulo, São Paulo, Brazil., Arns CW; Department of Genetics, Evolution and Bioagents, Institute of Biology, University of Campinas, Campinas, Brazil., Ferreira HL; Department of Veterinary Medicine, FZEA-USP, University of Sao Paulo, Pirassununga, Brazil.; Graduate Program in Experimental Epidemiology Applied to Zoonoses, FMVZ-USP, University of São Paulo, São Paulo, Brazil., Durigon EL; Institute of Biomedical Science, University of São Paulo, São Paulo, Brazil.
المصدر: Transboundary and emerging diseases [Transbound Emerg Dis] 2022 Sep; Vol. 69 (5), pp. e2863-e2875. Date of Electronic Publication: 2022 Jul 12.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Blackwell Verlag Country of Publication: Germany NLM ID: 101319538 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1865-1682 (Electronic) Linking ISSN: 18651674 NLM ISO Abbreviation: Transbound Emerg Dis Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Berlin : Blackwell Verlag
مواضيع طبية MeSH: Alphacoronavirus*/genetics , Chiroptera* , Coronavirus*/genetics , Coronavirus Infections*/veterinary, Amino Acids/genetics ; Animals ; Brazil/epidemiology ; Forests ; Genetic Variation ; Genome, Viral ; Phylogeny ; RNA-Dependent RNA Polymerase
مستخلص: Bat coronaviruses (Bat-CoVs) represent around 35% of all virus genomes described in bats. Brazil has one of the highest mammal species diversity, with 181 species of bats described so far. However, few Bat-CoV surveillance programmes were carried out in the country. Thus, our aim was to jevaluate the Bat-CoV diversity in the Atlantic Forest, the second biome with the highest number of bat species in Brazil. We analysed 456 oral and rectal swabs and 22 tissue samples from Atlantic Forest bats, detecting Alphacoronavirus in 44 swab samples (9.6%) targeting the RdRp gene from seven different bat species, three of which have never been described as Bat-CoV hosts. Phylogenetic analysis of the amino acid (aa) sequences coding the RdRp gene grouped the sequences obtained in our study with Bat-CoV previously detected in identical or congeneric bat species, belonging to four subgenera, with high aa identity (over 90%). The RdRp gene was also detected in three tissue samples from Diphylla ecaudata and Sturnira lilium, and the partial S gene was successfully sequenced in five tissues and swab samples of D. ecaudata. The phylogenetic analysis based on the partial S gene obtained here grouped the sequence of D. ecaudata with CoV from Desmodus rotundus previously detected in Peru and Brazil, belonging to the Amalacovirus subgenus, with aa identity ranging from 73.6% to 88.8%. Our data reinforce the wide distribution of Coronaviruses in bats from Brazil and the novelty of three bats species as Bat-CoV hosts and the co-circulation of four Alphacoronavirus subgenera in Brazil.
(© 2022 Wiley-VCH GmbH.)
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معلومات مُعتمدة: Ministry of Science, Technology, and Innovation (MCTI-Brazil); 403761/2020-4 National Council for Scientific and Technological Development; 400172/2022-4 National Council for Scientific and Technological Development; Brazilian Post and Telegraph Company (Correios)
فهرسة مساهمة: Keywords: RdRg gene; S gene; coronavirus; virus surveillance
المشرفين على المادة: 0 (Amino Acids)
EC 2.7.7.48 (RNA-Dependent RNA Polymerase)
تواريخ الأحداث: Date Created: 20220622 Date Completed: 20220929 Latest Revision: 20220929
رمز التحديث: 20231215
DOI: 10.1111/tbed.14636
PMID: 35729863
قاعدة البيانات: MEDLINE
الوصف
تدمد:1865-1682
DOI:10.1111/tbed.14636