دورية أكاديمية
Tissue engineering application combining epoxidized natural rubber blend and mesenchymal stem cells in in vivo response.
| العنوان: | Tissue engineering application combining epoxidized natural rubber blend and mesenchymal stem cells in in vivo response. |
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| المؤلفون: | Henckes NAC; Laboratório de Embriologia e Diferenciação Celular - Centro de Pesquisa Experimental, 37895Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.; Programa de Pós-graduação em Ciências da Saúde: Ginecologia e Obstetrícia, 28124Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil., Chuang L; Laboratório de Embriologia e Diferenciação Celular - Centro de Pesquisa Experimental, 37895Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil., Bosak I; Laboratório de Embriologia e Diferenciação Celular - Centro de Pesquisa Experimental, 37895Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil., Carazzai R; Laboratório de Biomateriais e Cerâmicas Avançadas, Departamento de Materiais, 28124Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil., Garcez T; Unidade de Experimentação Animal - Centro de Pesquisa Experimental, 37895Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil., Kuhl CP; Laboratório de Embriologia e Diferenciação Celular - Centro de Pesquisa Experimental, 37895Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.; Programa de Pós-graduação em Ciências da Saúde: Ginecologia e Obstetrícia, 28124Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil., de Oliveira FDS; Laboratório de Embriologia e Diferenciação Celular - Centro de Pesquisa Experimental, 37895Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil., Loureiro Dos Santos LA; Laboratório de Biomateriais e Cerâmicas Avançadas, Departamento de Materiais, 28124Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil., Visioli F; Unidade de Patologia Experimental - Centro de Pesquisa Experimental, 37895Hospital de Clinicas de Porto Alegre, Porto Alegre, Brazil.; Programa de Pós-graduação em Odontologia, 28124Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil., Cirne-Lima EO; Laboratório de Embriologia e Diferenciação Celular - Centro de Pesquisa Experimental, 37895Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.; Programa de Pós-graduação em Ciências da Saúde: Ginecologia e Obstetrícia, 28124Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.; Departamento de Patologia Clínica Veterinária, Faculdade de Veterinária, 28124Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil. |
| المصدر: | Journal of biomaterials applications [J Biomater Appl] 2022 Oct; Vol. 37 (4), pp. 698-711. Date of Electronic Publication: 2022 Jun 22. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Sage Publications Country of Publication: England NLM ID: 8813912 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1530-8022 (Electronic) Linking ISSN: 08853282 NLM ISO Abbreviation: J Biomater Appl Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2002- : London : Sage Publications Original Publication: Lancaster, PA : Technomic Pub. Co., Inc., [c1986- |
| مواضيع طبية MeSH: | Mesenchymal Stem Cells*/metabolism , Tissue Engineering*/methods, Animals ; Collagen/metabolism ; Female ; Ki-67 Antigen/metabolism ; Lactic Acid ; Polyglycolic Acid ; Polylactic Acid-Polyglycolic Acid Copolymer ; Rats ; Rubber/metabolism ; Tissue Scaffolds |
| مستخلص: | This study aimed to investigate biocompatibility, integration, and tissue host response of the Poly (Lactic-co-Glycolic acid) (PLGA)/Poly (isoprene) (PI) epoxidized (PLGA/PIepox) innovative scaffold combined with adipose derived mesenchymal stem cells (ADSC). We implanted the scaffold subcutaneously on the back of 18 female rats and monitored them for up to 14 days. When compared to controls, PLGA/PIepox + ADSC demonstrated an earlier vascularization, a tendency of inflammation reduction, an adequate tissue integration, higher cell proliferation, and a tendency of expression of collagen decreasing. However, 14 days post-implantation we found similar levels of CD31, Ki67 and AE1/AE3 in PLGA/PIepox when compared to control groups. The interesting results, lead us to the assumption that PLGA/PIepox is able to provide an effective delivery system for ADSC on tissue host. This animal study assesses PLGA/PIepox + ADSC in in vivo tissue functionality and validation of use, serving as a proof of concept for future clinical translation as it presents an innovative and promising tissue engineering opportunity for the use in tissue reconstruction. |
| فهرسة مساهمة: | Keywords: PLGA/PIepox; Tissue engineering; adipose derived mesenchymal stem cells; scaffolds |
| المشرفين على المادة: | 0 (Ki-67 Antigen) 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer) 26009-03-0 (Polyglycolic Acid) 33X04XA5AT (Lactic Acid) 9006-04-6 (Rubber) 9007-34-5 (Collagen) |
| تواريخ الأحداث: | Date Created: 20220623 Date Completed: 20221011 Latest Revision: 20221222 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1177/08853282221110476 |
| PMID: | 35733325 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1530-8022 |
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| DOI: | 10.1177/08853282221110476 |