دورية أكاديمية
Organotypic Rat Testicular Organoids for the Study of Testicular Maturation and Toxicology.
العنوان: | Organotypic Rat Testicular Organoids for the Study of Testicular Maturation and Toxicology. |
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المؤلفون: | Sakib S; Department of Biochemistry and Molecular Biology, Cumming School of Medicine, University of Calgary, Calgary, Canada.; Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary, Calgary, Canada., Lara NLEM; Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary, Calgary, Canada., Huynh BC; Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary, Calgary, Canada.; Department of Biological Sciences, Faculty of Science, University of Calgary, Calgary, Canada., Dobrinski I; Department of Biochemistry and Molecular Biology, Cumming School of Medicine, University of Calgary, Calgary, Canada.; Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary, Calgary, Canada. |
المصدر: | Frontiers in endocrinology [Front Endocrinol (Lausanne)] 2022 Jun 09; Vol. 13, pp. 892342. Date of Electronic Publication: 2022 Jun 09 (Print Publication: 2022). |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural |
اللغة: | English |
بيانات الدورية: | Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101555782 Publication Model: eCollection Cited Medium: Print ISSN: 1664-2392 (Print) Linking ISSN: 16642392 NLM ISO Abbreviation: Front Endocrinol (Lausanne) Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: [Lausanne : Frontiers Research Foundation] |
مواضيع طبية MeSH: | Organoids* , Spermatogonia*/metabolism, Animals ; Cell Differentiation ; Male ; Rats ; Testis/metabolism |
مستخلص: | An in vitro system to study testicular maturation in rats, an important model organism for reproductive toxicity, could serve as a platform for high-throughput drug and toxicity screening in a tissue specific context. In vitro maturation of somatic cells and spermatogonia in organ culture systems has been reported. However, this has been a challenge for organoids derived from dissociated testicular cells. Here, we report generation and maintenance of rat testicular organoids in microwell culture for 28 days. We find that rat organoids can be maintained in vitro only at lower than ambient O Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Sakib, Lara, Huynh and Dobrinski.) |
References: | J Toxicol Sci. 2015 Apr;40(2):151-61. (PMID: 25786521) Biol Reprod. 1998 Feb;58(2):470-4. (PMID: 9475403) Mol Endocrinol. 2013 Jan;27(1):63-73. (PMID: 23125070) J Androl. 2012 May-Jun;33(3):368-74. (PMID: 21868750) Oncotarget. 2015 Nov 10;6(35):37012-27. (PMID: 26473289) Toxicol In Vitro. 2014 Sep;28(6):1158-64. (PMID: 24909373) Biol Reprod. 1990 Feb;42(2):351-65. (PMID: 2186815) Sci Rep. 2016 Apr 11;6:24215. (PMID: 27062982) Int J Mol Sci. 2021 Dec 17;22(24):. (PMID: 34948348) Dev Biol. 2019 Jan 15;445(2):178-188. (PMID: 30391586) Nat Genet. 2001 Mar;27(3):271-6. (PMID: 11242108) Physiol Rev. 2012 Apr;92(2):577-95. (PMID: 22535892) Biol Reprod. 2010 Jan;82(1):202-13. (PMID: 19741204) Tohoku J Exp Med. 1986 May;149(1):79-87. (PMID: 3016947) Hum Reprod Update. 2017 Nov 1;23(6):660-680. (PMID: 28981651) Development. 2020 Jul 13;147(13):. (PMID: 32554530) Zhonghua Nan Ke Xue. 2013 Jan;19(1):29-34. (PMID: 23469658) Front Endocrinol (Lausanne). 2011 Apr 25;2:9. (PMID: 22649361) Hum Reprod. 2002 Jan;17(1):161-72. (PMID: 11756382) Andrology. 2020 Jul;8(4):879-891. (PMID: 31823507) Biofabrication. 2020 Jul 09;12(4):045002. (PMID: 32492667) Curr Res Toxicol. 2020 Mar 10;1:5-11. (PMID: 34345832) Toxicol Appl Pharmacol. 2009 Aug 1;238(3):240-9. (PMID: 19236889) J Occup Med Toxicol. 2006 Sep 10;1:22. (PMID: 16961932) Sci Rep. 2017 Nov 13;7(1):15459. (PMID: 29133858) mBio. 2019 Jul 16;10(4):. (PMID: 31311882) Sci Rep. 2017 Jul 24;7(1):6337. (PMID: 28740105) Endocrinology. 2010 Jun;151(6):2911-22. (PMID: 20357222) Philos Trans R Soc Lond B Biol Sci. 2010 May 27;365(1546):1697-712. (PMID: 20403879) Brain Plast. 2020 Oct 01;5(2):135-145. (PMID: 33282677) FASEB J. 2020 Aug;34(8):10373-10386. (PMID: 32557858) Adv Exp Med Biol. 1995;377:33-54. (PMID: 7484435) Nature. 2011 Mar 24;471(7339):504-7. (PMID: 21430778) Int J Mol Sci. 2021 Jul 31;22(15):. (PMID: 34361033) Philos Trans R Soc Lond B Biol Sci. 2009 Jul 27;364(1526):2079-96. (PMID: 19528057) Mol Hum Reprod. 2010 Feb;16(2):97-110. (PMID: 19770206) Nature. 2003 Aug 21;424(6951):870-2. (PMID: 12931155) J Reprod Fertil. 1981 Nov;63(2):373-9. (PMID: 7299745) Arch Androl. 1987;19(2):127-32. (PMID: 3435194) Environ Pollut. 2021 Sep 1;284:117446. (PMID: 34058501) Spermatogenesis. 2015 Jan 21;4(2):e981485. (PMID: 26413399) PLoS One. 2014 Dec 04;9(12):e113793. (PMID: 25474107) Biol Reprod. 2019 Jun 1;100(6):1648-1660. (PMID: 30927418) Fertil Steril. 2018 Nov;110(6):1045-1057.e3. (PMID: 30396549) Methods Mol Biol. 2014;1154:563-78. (PMID: 24782029) Sci Rep. 2021 Feb 10;11(1):3458. (PMID: 33568686) Rev Reprod. 1999 Jan;4(1):23-30. (PMID: 10051099) Biofabrication. 2019 Apr 26;11(3):035011. (PMID: 30921781) Reprod Domest Anim. 2015 Dec;50(6):1032-8. (PMID: 26482643) Int J Mol Sci. 2021 Jan 24;22(3):. (PMID: 33498962) J Cell Physiol. 2018 Apr;233(4):3629-3637. (PMID: 29034469) Environ Res. 2008 Oct;108(2):168-76. (PMID: 18949836) Acta Histochem Cytochem. 2021 Oct 29;54(5):167-175. (PMID: 34764525) PLoS One. 2011;6(11):e27172. (PMID: 22087261) CNS Neurosci Ther. 2020 Oct;26(10):1058-1068. (PMID: 32562453) Int J Mol Med. 2018 May;41(5):2629-2639. (PMID: 29484379) PLoS One. 2018 Oct 16;13(10):e0204269. (PMID: 30325922) Biol Reprod. 2017 Mar 1;96(3):720-732. (PMID: 28339648) J Androl. 2001 Nov-Dec;22(6):911-26. (PMID: 11700854) Biomaterials. 2017 Jun;130:76-89. (PMID: 28364632) J Hum Reprod Sci. 2015 Oct-Dec;8(4):191-6. (PMID: 26752853) J Endocrinol. 2007 Sep;194(3):579-93. (PMID: 17761897) Mol Cell. 2000 Jan;5(1):73-83. (PMID: 10678170) Adv Exp Med Biol. 2008;636:1-15. (PMID: 19856159) |
معلومات مُعتمدة: | R01 HD091068 United States HD NICHD NIH HHS; R01 OD016575 United States OD NIH HHS |
فهرسة مساهمة: | Keywords: MEHP; Sertoli cell; cadmium chloride; spermatogonia; testicular organoid |
تواريخ الأحداث: | Date Created: 20220627 Date Completed: 20220628 Latest Revision: 20220729 |
رمز التحديث: | 20221213 |
مُعرف محوري في PubMed: | PMC9218276 |
DOI: | 10.3389/fendo.2022.892342 |
PMID: | 35757431 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1664-2392 |
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DOI: | 10.3389/fendo.2022.892342 |