دورية أكاديمية
أعرض في EDS

Organotypic Rat Testicular Organoids for the Study of Testicular Maturation and Toxicology.

التفاصيل البيبلوغرافية
العنوان: Organotypic Rat Testicular Organoids for the Study of Testicular Maturation and Toxicology.
المؤلفون: Sakib S; Department of Biochemistry and Molecular Biology, Cumming School of Medicine, University of Calgary, Calgary, Canada.; Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary, Calgary, Canada., Lara NLEM; Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary, Calgary, Canada., Huynh BC; Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary, Calgary, Canada.; Department of Biological Sciences, Faculty of Science, University of Calgary, Calgary, Canada., Dobrinski I; Department of Biochemistry and Molecular Biology, Cumming School of Medicine, University of Calgary, Calgary, Canada.; Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary, Calgary, Canada.
المصدر: Frontiers in endocrinology [Front Endocrinol (Lausanne)] 2022 Jun 09; Vol. 13, pp. 892342. Date of Electronic Publication: 2022 Jun 09 (Print Publication: 2022).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural
اللغة: English
بيانات الدورية: Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101555782 Publication Model: eCollection Cited Medium: Print ISSN: 1664-2392 (Print) Linking ISSN: 16642392 NLM ISO Abbreviation: Front Endocrinol (Lausanne) Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Lausanne : Frontiers Research Foundation]
مواضيع طبية MeSH: Organoids* , Spermatogonia*/metabolism, Animals ; Cell Differentiation ; Male ; Rats ; Testis/metabolism
مستخلص: An in vitro system to study testicular maturation in rats, an important model organism for reproductive toxicity, could serve as a platform for high-throughput drug and toxicity screening in a tissue specific context. In vitro maturation of somatic cells and spermatogonia in organ culture systems has been reported. However, this has been a challenge for organoids derived from dissociated testicular cells. Here, we report generation and maintenance of rat testicular organoids in microwell culture for 28 days. We find that rat organoids can be maintained in vitro only at lower than ambient O 2 tension of 15% and organoids cultured at 34°C have higher somatic cell maturation and spermatogonial differentiation potential compared to cultures in 37°C. Upon exposure to known toxicants, phthalic acid mono-2-ethylhexyl ester and cadmium chloride, the organoids displayed loss of tight-junction protein Claudin 11 and altered transcription levels of somatic cell markers that are consistent with previous reports in animal models. Therefore, the microwell-derived rat testicular organoids described here can serve as a novel platform for the study of testicular cell maturation and reproductive toxicity in vitro .
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2022 Sakib, Lara, Huynh and Dobrinski.)
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معلومات مُعتمدة: R01 HD091068 United States HD NICHD NIH HHS; R01 OD016575 United States OD NIH HHS
فهرسة مساهمة: Keywords: MEHP; Sertoli cell; cadmium chloride; spermatogonia; testicular organoid
تواريخ الأحداث: Date Created: 20220627 Date Completed: 20220628 Latest Revision: 20220729
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC9218276
DOI: 10.3389/fendo.2022.892342
PMID: 35757431
قاعدة البيانات: MEDLINE
الوصف
تدمد:1664-2392
DOI:10.3389/fendo.2022.892342