دورية أكاديمية
أعرض في EDS

Genome analysis and virulence gene expression profile of a multi drug resistant Salmonella enterica serovar Typhimurium ms202.

التفاصيل البيبلوغرافية
العنوان: Genome analysis and virulence gene expression profile of a multi drug resistant Salmonella enterica serovar Typhimurium ms202.
المؤلفون: Mohakud NK; School of Biotechnology, KIIT University, Bhubaneswar, 751024, India.; Kalinga Institute of Medical Sciences (KIMS), KIIT University, Bhubaneswar, 751024, India., Panda RK; SCB Medical College, Cuttack, India., Patra SD; School of Biotechnology, KIIT University, Bhubaneswar, 751024, India., Sahu BR; School of Biotechnology, KIIT University, Bhubaneswar, 751024, India., Ghosh M; KIIT-Technology Business Incubator (KIIT-TBI), KIIT University, Bhubaneswar, 751024, India. mringhs2010@gmail.com., Kushwaha GS; KIIT-Technology Business Incubator (KIIT-TBI), KIIT University, Bhubaneswar, 751024, India., Misra N; KIIT-Technology Business Incubator (KIIT-TBI), KIIT University, Bhubaneswar, 751024, India., Suar M; School of Biotechnology, KIIT University, Bhubaneswar, 751024, India. mrutyunjay@kiitincubator.in.; KIIT-Technology Business Incubator (KIIT-TBI), KIIT University, Bhubaneswar, 751024, India. mrutyunjay@kiitincubator.in.
المصدر: Gut pathogens [Gut Pathog] 2022 Jun 28; Vol. 14 (1), pp. 28. Date of Electronic Publication: 2022 Jun 28.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101474263 Publication Model: Electronic Cited Medium: Print ISSN: 1757-4749 (Print) Linking ISSN: 17574749 NLM ISO Abbreviation: Gut Pathog Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central
مستخلص: Background: In India, multi-drug resistance in Salmonella enterica serovar Typhimurium poses a significant health threat. Indeed, S. Typhimurium has remained unknown for a large portion of its genome associated with various physiological functions including mechanism of drug resistance and virulence. The whole-genome sequence of a Salmonella strain obtained from feces of a patient with gastroenteritis in Odisha, India, was analyzed for understanding the disease association and underlying virulence mechanisms.
Results: The de novo assembly yielded 17 contigs and showed 99.9% similarity to S. enterica sub sp enterica strain LT2 and S. enteric subsp salamae strain DSM 9220. S. Typhimurium ms202 strain constitutes six known Salmonella pathogenicity islands and nine different phages. The comparative interpretation of pathogenic islands displayed the genes contained in SPI-1 and SPI-2 to be highly conserved. We identified sit ABCD cluster regulatory cascade in SPI-1. Multiple antimicrobial resistance genes were identified that directly implies antibiotic-resistant phenotype. Notably, seven unique genes were identified as "acquired antibiotic resistance". These data suggest that virulence in S. enterica Typhimurium ms202 is associated with SPI-1 and SPI-2. Further, we found several virulent genes encoding SPI regions belonging to type III secretion systems (T3SS) of bacteria were significantly upregulated in ms202 compared to control LT2. Moreover, all these genes were significantly downregulated in S. enterica Typhimurium ms202 as compared to control LT2 on adding Mn 2+ exogenously.
Conclusions: Our study raises a vital concern about the potential diffusion of a novel multi-drug resistant S. enterica Typhimurium ms202. It justifies this clinical pathogen to demonstrate a higher degree survival due to higher expression of virulent genes and enhanced ability of metallic ion acquisition.
(© 2022. The Author(s).)
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فهرسة مساهمة: Keywords: Salmonella Typhimurium; Virulence; Whole genome sequence; ms202
تواريخ الأحداث: Date Created: 20220628 Latest Revision: 20220716
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC9237969
DOI: 10.1186/s13099-022-00498-w
PMID: 35765034
قاعدة البيانات: MEDLINE
الوصف
تدمد:1757-4749
DOI:10.1186/s13099-022-00498-w