دورية أكاديمية
أعرض في EDS

Extended-spectrum-beta-lactamases and carbapenemase-producing Klebsiella pneumoniae isolated from fresh produce farms in different governorates of Egypt.

التفاصيل البيبلوغرافية
العنوان: Extended-spectrum-beta-lactamases and carbapenemase-producing Klebsiella pneumoniae isolated from fresh produce farms in different governorates of Egypt.
المؤلفون: Elshafiee EA; Department of Zoonoses, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt., Kadry M; Department of Zoonoses, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt., Nader SM; Department of Zoonoses, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt., Ahmed ZS; Department of Zoonoses, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt.
المصدر: Veterinary world [Vet World] 2022 May; Vol. 15 (5), pp. 1191-1196. Date of Electronic Publication: 2022 May 18.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Veterinary World Country of Publication: India NLM ID: 101504872 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0972-8988 (Print) Linking ISSN: 09728988 NLM ISO Abbreviation: Vet World Subsets: PubMed not MEDLINE
أسماء مطبوعة: Publication: Dist. Morbi (Gujarat), India : Veterinary World
Original Publication: Rajkot (Gujarat), India : Veterinary World, [2008]-
مستخلص: Background and Aim: Fresh produce farms represents a major source of concern since they are becoming increasingly antibiotic resistant. This study aimed to investigate the occurrence of carbapenemase and extended-spectrum-beta-lactamases (ESBL) - producing genes in Klebsiella pneumonia e isolated from fresh produce farms in Egypt, irrigation water, and people working in these fields.
Materials and Methods: One hundred tomatoes from typical farms were collected in plastic bags. The study also included 20 surface-water samples from different irrigation watersheds in fresh produce farms, as well as 50 feces samples from farmworkers. Suspected K. pneumoniae was grown on Eosin Methylene Blue agar for 24 h before being biochemically identified using the RapID ONE test. PCR was used to detect carbapenemase ( bla KPC, bla OXA-48, and bla NDM) and ESBL ( bla SHV, bla TEM, and bla CTX) expressing genes on isolates.
Results: K . pneumoniae was identified in 30% of water and 10% of worker samples, while only one isolate was found in tomato samples. One of the six irrigation water isolates tested positive for carbapenem-resistant NDM. In contrast, two isolates tested positive for ESBL determinants, one of which was bl SHV and the other having both bla SHV and bla TEM genes. Two of the five K. pneumoniae isolates from farmworkers were positive for bla NDM, with one isolate also testing positive for bla SHV and bla TEM. The bla OXA-48 gene was also discovered in the carbapenem-resistant K. pneumoniae tomato isolate used in this study.
Conclusion: Carbapenemase- and ESBL-producing K. pneumoniae were found in fresh produce farms, implying that these resistance genes were being passed down to Egyptian consumers.
Competing Interests: The authors declare that they have no competing interests.
(Copyright: © Elshafiee, et al.)
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فهرسة مساهمة: Keywords: Klebsiella pneumonia; carbapenemase; extended-spectrum-beta-lactamases; fresh produce; humans; irrigation water
تواريخ الأحداث: Date Created: 20220629 Latest Revision: 20220716
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC9210849
DOI: 10.14202/vetworld.2022.1191-1196
PMID: 35765488
قاعدة البيانات: MEDLINE
الوصف
تدمد:0972-8988
DOI:10.14202/vetworld.2022.1191-1196