Immunological quantitative detection of dicofol in medicinal materials.

التفاصيل البيبلوغرافية
العنوان:	Immunological quantitative detection of dicofol in medicinal materials.
المؤلفون:	Lin L; State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China. xcl@jiangnan.edu.cn., Xu X; State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China. xcl@jiangnan.edu.cn., Song S; State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China. xcl@jiangnan.edu.cn., Xu L; State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China. xcl@jiangnan.edu.cn., Zhu Y; School of Biotechnology and Food Engineering, Changshu Institute of Technology, Changshu, Jiangsu 215500, China. yyzhujnu@163.com., Kuang H; State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China. xcl@jiangnan.edu.cn., Liu L; State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China. xcl@jiangnan.edu.cn., Xu C; State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China. xcl@jiangnan.edu.cn.
المصدر:	The Analyst [Analyst] 2022 Jul 22; Vol. 147 (15), pp. 3478-3485. Date of Electronic Publication: 2022 Jul 22.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 0372652 Publication Model: Electronic Cited Medium: Internet ISSN: 1364-5528 (Electronic) Linking ISSN: 00032654 NLM ISO Abbreviation: Analyst Subsets: MEDLINE
أسماء مطبوعة:	Publication: Cambridge : Royal Society of Chemistry Original Publication: London : Chemical Society
مواضيع طبية MeSH:	Dicofol* , Gold Colloid*/chemistry, Chromatography, Affinity/methods ; Immunoassay/methods ; Limit of Detection
مستخلص:	In this study, a highly specific and sensitive monoclonal antibody against dicofol (DIC) was prepared and used to create a colloidal gold immunochromatographic strip (ICS) for the quantitative analysis of DIC in liquorice and Angelica sinensis samples. We found that for both the liquorice and Angelica sinensis samples, the cut-off value for the ICS was 500 ng g ^-1 , respectively, and using a colloidal gold test strip reader, the half-maximal inhibitory concentrations (IC 50 ) were found to be 35 ng g ^-1 and 88 ng g ^-1 , the limits of detection were 2 ng g ^-1 and 3 ng g ^-1 , and the linear detection scopes (IC 20 -IC 80 ) were from 6 to 201 ng g ^-1 and from 10 to 819 ng g ^-1 , respectively. In the recovery test, we found that recoveries were within the range of 90.6% to 97.2% and the coefficients of variation were less than 8.6% in the intra-assay and inter-assay for the liquorice samples and the recoveries ranged from 90.8% to 97.3% and the coefficients of variation were less than 9.1% in the intra-assay and inter-assay for the Angelica sinensis samples. The established ICS assay could be used for the rapid quantitative screening of chemicals in Chinese traditional medicinal materials.
المشرفين على المادة:	0 (Gold Colloid) W4WMM0WS91 (Dicofol)
تواريخ الأحداث:	Date Created: 20220629 Date Completed: 20220726 Latest Revision: 20230410
رمز التحديث:	20231215
DOI:	10.1039/d2an00462c
PMID:	35766970
قاعدة البيانات:	MEDLINE

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الوصف
تدمد:	1364-5528
DOI:	10.1039/d2an00462c