دورية أكاديمية
أعرض في EDS

The Role of Egg Yolk in Modulating the Virulence of Salmonella Enterica Serovar Enteritidis.

التفاصيل البيبلوغرافية
العنوان: The Role of Egg Yolk in Modulating the Virulence of Salmonella Enterica Serovar Enteritidis.
المؤلفون: Xu Y; Department of Food Science and Technology, The Ohio State University, Columbus, OH, United States., Abdelhamid AG; Department of Food Science and Technology, The Ohio State University, Columbus, OH, United States.; Botany and Microbiology Department, Faculty of Science, Benha University, Benha, Egypt., Sabag-Daigle A; Department of Microbial Infection and Immunity, The Ohio State University, Columbus, OH, United States., Sovic MG; Center for Applied Plant Sciences, The Ohio State University, Columbus, OH, United States., Ahmer BMM; Department of Microbial Infection and Immunity, The Ohio State University, Columbus, OH, United States., Yousef AE; Department of Food Science and Technology, The Ohio State University, Columbus, OH, United States.; Department of Microbiology, The Ohio State University, Columbus, OH, United States.
المصدر: Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2022 Jun 14; Vol. 12, pp. 903979. Date of Electronic Publication: 2022 Jun 14 (Print Publication: 2022).
نوع المنشور: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: Frontiers Media SA Country of Publication: Switzerland NLM ID: 101585359 Publication Model: eCollection Cited Medium: Internet ISSN: 2235-2988 (Electronic) Linking ISSN: 22352988 NLM ISO Abbreviation: Front Cell Infect Microbiol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Lausanne : Frontiers Media SA
مواضيع طبية MeSH: Salmonella Infections* , Salmonella Infections, Animal*/microbiology, Animals ; Chickens/microbiology ; Egg Yolk/microbiology ; Mice ; Salmonella enteritidis/genetics ; Virulence/genetics
مستخلص: Contribution of food vehicles to pathogenicity of disease-causing microorganisms is an important but overlooked research field. The current study was initiated to reveal the relationship between virulence of Salmonella enterica serovar Enteritidis and egg yolk as a hosting medium. Mice were orally challenged with Salmonella Enteritidis cultured in egg yolk or tryptic soy broth (TSB). Additionally, mice were challenged with Salmonella Enteritidis cultured in TSB, followed by administration of sterile egg yolk, to discern the difference between pre-growth of the pathogen and its mere presence in egg yolk during infection. The pathogen's Lethal dose 50 (LD 50 ) was the lowest when grown in yolk (2.8×10 2 CFU), compared to 1.1×10 3 CFU in TSB, and 4.6×10 3 CFU in TSB followed by administration of sterile yolk. Additionally, mice that orally received Salmonella Enteritidis grown in egg yolk expressed a high death rate. These findings were supported by transcriptional analysis results. Expression of promoters of virulence-related genes ( sopB and sseA ) in genetically modified Salmonella Enteritidis reporter strains was significantly higher ( p < 0.05) when the bacterium was grown in the yolk, compared to that grown in TSB. Sequencing of RNA (RNA-seq) revealed 204 differentially transcribed genes in Salmonella Enteritidis grown in yolk vs . TSB. Yolk-grown Salmonella Enteritidis exhibited upregulated virulence pathways, including type III secretion systems, epithelial cell invasion, and infection processes; these observations were confirmed by RT-qPCR results. The transcriptomic analysis suggested that upregulation of virulence machinery of Salmonella Enteritidis grown in egg yolk was related to increased iron uptake, biotin utilization, flagellar biosynthesis, and export of virulence proteins encoded on Salmonella pathogenicity island 1, 2, 4, and 5. These biological responses may have acted in concert to increase the virulence of Salmonella infection in mice. In conclusion, growth in egg yolk enhanced Salmonella Enteritidis virulence, indicating the significance of this food vehicle to the risk assessment of salmonellosis.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2022 Xu, Abdelhamid, Sabag-Daigle, Sovic, Ahmer and Yousef.)
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فهرسة مساهمة: Keywords: RNA sequencing; Salmonella Enteritidis; mouse model; stress response; transcriptomic analysis; virulence
تواريخ الأحداث: Date Created: 20220701 Date Completed: 20220704 Latest Revision: 20220809
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC9237210
DOI: 10.3389/fcimb.2022.903979
PMID: 35774398
قاعدة البيانات: MEDLINE
الوصف
تدمد:2235-2988
DOI:10.3389/fcimb.2022.903979