دورية أكاديمية
أعرض في EDS

Prevalence and Molecular Characterization of Metallo β-Lactamase Producing Gram-Negative Pathogens Causing Eye Infections.

التفاصيل البيبلوغرافية
العنوان: Prevalence and Molecular Characterization of Metallo β-Lactamase Producing Gram-Negative Pathogens Causing Eye Infections.
المؤلفون: Rameshkumar G; Medical Microbiology Laboratory, Department of Microbiology, Alagappa University, Karaikudi, India.; Department of Ocular Microbiology, Aravind Eye Hospital, Madurai, India., Dhandapani R; Medical Microbiology Laboratory, Department of Microbiology, Alagappa University, Karaikudi, India., Lalitha P; Department of Ocular Microbiology, Aravind Eye Hospital, Madurai, India., Rajapandian SGK; Department of Ocular Microbiology, Aravind Eye Hospital, Madurai, India., Palanivel V; Centre for Material Engineering and Regenerative Medicine Bharath Institute of Higher Education, Chennai, India., Thangavelu S; Medical Microbiology Laboratory, Department of Microbiology, Alagappa University, Karaikudi, India., Alyousef AA; Clinical Laboratory Sciences Department, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia., Albalawi T; Department of Biology, College of Sciences and Humanities, Prince Sattam Bin Andulaziz University, Alkharj, Saudi Arabia., Alam P; Department of Biology, College of Sciences and Humanities, Prince Sattam Bin Andulaziz University, Alkharj, Saudi Arabia., Zubair M; Department of Medical Microbiology, Faculty of Medicine, University of Tabuk, Tabuk, Saudi Arabia., Saleh FM; Department of Medical Microbiology, Faculty of Medicine, University of Tabuk, Tabuk, Saudi Arabia., Alatawi FA; Department of Biology, Faculty of Science, University of Tabuk, Tabuk, Saudi Arabia., Husain FM; Department of Food Science and Nutrition, King Saud University, Riyadh, Saudi Arabia.
المصدر: Frontiers in public health [Front Public Health] 2022 Jun 14; Vol. 10, pp. 870354. Date of Electronic Publication: 2022 Jun 14 (Print Publication: 2022).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Editorial Office Country of Publication: Switzerland NLM ID: 101616579 Publication Model: eCollection Cited Medium: Internet ISSN: 2296-2565 (Electronic) Linking ISSN: 22962565 NLM ISO Abbreviation: Front Public Health Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Lausanne : Frontiers Editorial Office
مواضيع طبية MeSH: Endophthalmitis*/drug therapy , Endophthalmitis*/epidemiology , Eye Infections*/drug therapy, Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Escherichia coli ; Gram-Negative Bacteria/genetics ; Humans ; Microbial Sensitivity Tests ; Prevalence ; Pseudomonas aeruginosa/genetics ; beta-Lactamases/genetics
مستخلص: Purpose: Metallo β-lactamases (MβL) production is a worldwide problem, particularly in gram-negative bacteria. As scanty data is available on the prevalence of MBL, the present study is being undertaken to determine the prevalence, antibacterial sensitivity patterns, and molecular characterization of MβL associated resistant genes in gram-negative bacteria isolated from ocular infections.
Material and Methods: At a tertiary eye care center in south India, 359 gram-negative pathogens, 200 isolates from eye infections, and 159 isolates from normal flora of the eye were studied. A gold standard microbiology method was used to identify the isolates. An antibiotic double disc synergy test and a combination disc test were used to detect MβL production. Multiplex PCR was used to investigate the molecular characteristics of the MβL encoding genes bla VIM , bla IMP , and bla NDM .
Results: Of the 359 gram-negative bacterial pathogens, Pseudomonas aeruginosa 108 (30.1%) and Enterobacter agglomerans 46 (12.8%) were commonly isolated. High prevalence of P. aeruginosa 81% (17 strains) was detected as an MβL producer and it shows 100% resistance to 2nd and 3rd generation cephalosporins and meropenem. Multiplex PCR detected only the bla VIM gene in 56 (28%) of various eye infections and 27 (17%) of normal flora of the gram-negative bacteria (GNB). The bla VIM gene is detected predominantly in 51.8% of keratitis and 21.4% of postoperative endophthalmitis. High prevalence of the gene was detected in P. aeruginosa 42.9% (24 of 56) and Alcaligens denitrificans 10.7% (6 of 56) from eye infections. Whereas, in the control group, P. aeruginosa and E. coli each had 14.8% (4 of 27) that were shown positive.
Conclusion: The emerging MβLs mediated resistance among P. aeruginosa is a challenging task for ophthalmologists, especially in patients with endophthalmitis and bacterial keratitis. This local knowledge will aid in advising appropriate antibiotic use and avoiding unnecessary antibiotic prescriptions, which are highly warranted.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2022 Rameshkumar, Dhandapani, Lalitha, Rajapandian, Palanivel, Thangavelu, Alyousef, Albalawi, Alam, Zubair, Saleh, Alatawi and Husain.)
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فهرسة مساهمة: Keywords: Enterobacteriaceae; Metallo β-lactamases; gram-negative bacilli; non-Enterobacteriaceae; normal flora
المشرفين على المادة: 0 (Anti-Bacterial Agents)
EC 3.5.2.6 (beta-Lactamases)
تواريخ الأحداث: Date Created: 20220701 Date Completed: 20220704 Latest Revision: 20220803
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC9237426
DOI: 10.3389/fpubh.2022.870354
PMID: 35774580
قاعدة البيانات: MEDLINE
الوصف
تدمد:2296-2565
DOI:10.3389/fpubh.2022.870354