دورية أكاديمية
أعرض في EDS

Aedes aegypti Aag-2 Cell Proteome Modulation in Response to Chikungunya Virus Infection.

التفاصيل البيبلوغرافية
العنوان: Aedes aegypti Aag-2 Cell Proteome Modulation in Response to Chikungunya Virus Infection.
المؤلفون: Vasconcellos AF; Laboratory of Biochemistry and Protein Chemistry, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, Brazil.; Laboratory of Virology, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, Brazil., Melo RM; Laboratory of Biochemistry and Protein Chemistry, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, Brazil., Mandacaru SC; Laboratory of Toxinology and Center for Technological Development in Health, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil., de Oliveira LS; Laboratory of Biochemistry and Protein Chemistry, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, Brazil., de Oliveira AS; Laboratory of Virology, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, Brazil., Moraes ECDS; Laboratory of Toxinology and Center for Technological Development in Health, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil., Trugilho MRO; Laboratory of Toxinology and Center for Technological Development in Health, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil., Ricart CAO; Laboratory of Biochemistry and Protein Chemistry, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, Brazil., Báo SN; Laboratory of Microscopy and Microanalysis, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, Brazil., Resende RO; Laboratory of Virology, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, Brazil., Charneau S; Laboratory of Biochemistry and Protein Chemistry, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, Brazil.
المصدر: Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2022 Jun 15; Vol. 12, pp. 920425. Date of Electronic Publication: 2022 Jun 15 (Print Publication: 2022).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Frontiers Media SA Country of Publication: Switzerland NLM ID: 101585359 Publication Model: eCollection Cited Medium: Internet ISSN: 2235-2988 (Electronic) Linking ISSN: 22352988 NLM ISO Abbreviation: Front Cell Infect Microbiol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Lausanne : Frontiers Media SA
مواضيع طبية MeSH: Aedes* , Chikungunya Fever* , Chikungunya virus*, Animals ; Humans ; Mosquito Vectors ; Proteome ; Proteomics
مستخلص: Chikungunya virus (CHIKV) is a single-stranded positive RNA virus that belongs to the genus Alphavirus and is transmitted to humans by infected Aedes aegypti and Aedes albopictus bites. In humans, CHIKV usually causes painful symptoms during acute and chronic stages of infection. Conversely, virus-vector interaction does not disturb the mosquito's fitness, allowing a persistent infection. Herein, we studied CHIKV infection of Ae. aegypti Aag-2 cells (multiplicity of infection (MOI) of 0.1) for 48 h through label-free quantitative proteomic analysis and transmission electron microscopy (TEM). TEM images showed a high load of intracellular viral cargo at 48 h postinfection (hpi), as well as an unusual elongated mitochondria morphology that might indicate a mitochondrial imbalance. Proteome analysis revealed 196 regulated protein groups upon infection, which are related to protein synthesis, energy metabolism, signaling pathways, and apoptosis. These Aag-2 proteins regulated during CHIKV infection might have roles in antiviral and/or proviral mechanisms and the balance between viral propagation and the survival of host cells, possibly leading to the persistent infection.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2022 Vasconcellos, Melo, Mandacaru, de Oliveira, de Oliveira, Moraes, Trugilho, Ricart, Báo, Resende and Charneau.)
References: Annu Rev Biochem. 2016 Jun 2;85:103-32. (PMID: 27023846)
Sci Rep. 2021 Dec 8;11(1):23696. (PMID: 34880409)
Front Physiol. 2018 May 24;9:575. (PMID: 29881355)
Front Physiol. 2019 Jan 11;9:1912. (PMID: 30687124)
Cell Death Dis. 2016 Mar 03;7:e2127. (PMID: 26938301)
Nucleic Acids Res. 2019 Jan 8;47(D1):D442-D450. (PMID: 30395289)
Annu Rev Microbiol. 2012;66:305-23. (PMID: 22746336)
Rev Soc Bras Med Trop. 2014 Nov-Dec;47(6):677-83. (PMID: 25626645)
Trends Cell Biol. 2020 Apr;30(4):263-275. (PMID: 32200805)
PLoS Negl Trop Dis. 2010 Oct 26;4(10):e856. (PMID: 21049065)
Virology. 2018 Feb;515:223-234. (PMID: 29306785)
Arch Virol. 2019 Apr;164(4):1205-1208. (PMID: 30729309)
mBio. 2019 Mar 12;10(2):. (PMID: 30862747)
BMC Syst Biol. 2010 May 13;4:61. (PMID: 20465796)
Bioinformatics. 2018 May 1;34(9):1600-1602. (PMID: 29069305)
Microbiol Rev. 1994 Sep;58(3):491-562. (PMID: 7968923)
Virusdisease. 2021 Dec;32(4):657-665. (PMID: 34901322)
J Proteome Res. 2013 Mar 1;12(3):1377-86. (PMID: 23323968)
J Med Virol. 2012 Nov;84(11):1757-70. (PMID: 22997079)
Proc Natl Acad Sci U S A. 2019 Sep 17;116(38):19136-19144. (PMID: 31488709)
Biochim Biophys Acta. 2013 Aug;1829(8):854-65. (PMID: 23567047)
Trends Microbiol. 2022 Jul;30(7):679-692. (PMID: 35063304)
J Virol. 2011 Jan;85(1):606-20. (PMID: 20962078)
J Virol. 2009 Jun;83(11):5815-24. (PMID: 19297497)
Lancet Infect Dis. 2017 Apr;17(4):e107-e117. (PMID: 28159534)
Genes Dev. 2008 Feb 15;22(4):476-88. (PMID: 18281461)
Front Cell Infect Microbiol. 2018 Jan 17;7:542. (PMID: 29387631)
PLoS Negl Trop Dis. 2015 Mar 04;9(3):e0003544. (PMID: 25738640)
J Proteome Res. 2019 Feb 1;18(2):623-632. (PMID: 30450911)
Trends Microbiol. 2013 Aug;21(8):360-3. (PMID: 23910545)
Viruses. 2018 Feb 22;10(2):. (PMID: 29470397)
Front Cell Infect Microbiol. 2019 Jan 07;8:440. (PMID: 30666300)
J Proteome Res. 2020 Jun 5;19(6):2443-2456. (PMID: 32375005)
PLoS Pathog. 2010 Jul 15;6(7):e1000986. (PMID: 20657822)
Int J Biochem Cell Biol. 2013 Jan;45(1):41-6. (PMID: 23036789)
J Proteome Res. 2018 Jan 5;17(1):386-391. (PMID: 29083185)
Parasit Vectors. 2012 Nov 15;5:264. (PMID: 23153178)
Virus Res. 2021 Apr 15;296:198352. (PMID: 33640359)
Nat Rev Microbiol. 2011 Oct 17;9(12):860-75. (PMID: 22002165)
Cells. 2019 May 27;8(5):. (PMID: 31137833)
J Proteome Res. 2018 May 4;17(5):1844-1851. (PMID: 29578715)
Virology. 2010 Oct 10;406(1):149-61. (PMID: 20674955)
Mol Cell. 2001 Aug;8(2):251-62. (PMID: 11545728)
Parasit Vectors. 2020 Jun 10;13(1):297. (PMID: 32522239)
Viruses. 2020 Feb 05;12(2):. (PMID: 32033386)
BMC Bioinformatics. 2009 May 27;10:161. (PMID: 19473525)
Biochim Biophys Acta. 1998 Aug 10;1366(1-2):139-49. (PMID: 9714780)
mBio. 2018 Mar 6;9(2):. (PMID: 29511072)
BMC Biol. 2019 Jul 18;17(1):59. (PMID: 31319842)
J Cell Mol Med. 2006 Apr-Jun;10(2):353-63. (PMID: 16796804)
PLoS Negl Trop Dis. 2013;7(2):e2050. (PMID: 23409203)
J Gen Virol. 2021 Apr;102(4):. (PMID: 33904816)
J Proteome Res. 2018 Oct 5;17(10):3348-3359. (PMID: 30192139)
J Exp Med. 2012 May 7;209(5):1029-47. (PMID: 22508836)
Bioinformatics. 2009 Feb 15;25(4):555-6. (PMID: 19129209)
Nucleic Acids Res. 2019 Jan 8;47(D1):D607-D613. (PMID: 30476243)
PLoS Negl Trop Dis. 2019 Jan 3;13(1):e0006822. (PMID: 30605475)
Bioinformatics. 2015 Nov 15;31(22):3718-20. (PMID: 26209431)
J Cell Sci. 2010 Oct 1;123(Pt 19):3209-14. (PMID: 20844150)
Mitochondrion. 2018 Jul;41:21-27. (PMID: 29246869)
J Cell Physiol. 2014 Aug;229(8):998-1004. (PMID: 24347342)
Cell Host Microbe. 2016 Sep 14;20(3):342-356. (PMID: 27545046)
J Proteome Res. 2019 Mar 1;18(3):1418-1425. (PMID: 30638385)
Virol Sin. 2021 Dec;36(6):1532-1542. (PMID: 34519916)
Virology. 2009 Oct 25;393(2):183-97. (PMID: 19732931)
Annu Rev Biochem. 2004;73:87-106. (PMID: 15189137)
Nucleic Acids Res. 2021 Jan 8;49(D1):D480-D489. (PMID: 33237286)
Mol Omics. 2021 Jun 14;17(3):376-393. (PMID: 34125125)
Curr Opin Struct Biol. 2002 Feb;12(1):123-33. (PMID: 11839499)
Rev Saude Publica. 2015;49:58. (PMID: 26398876)
Viruses. 2019 Nov 19;11(11):. (PMID: 31752346)
Bioinorg Chem Appl. 2011;2011:363941. (PMID: 21716720)
Mol Cell. 2003 Jan;11(1):127-38. (PMID: 12535527)
Bioinformatics. 2018 Sep 1;34(17):2965-2972. (PMID: 29635359)
J Biol Chem. 2003 Sep 19;278(38):36027-31. (PMID: 12840017)
Trop Med Health. 2011 Dec;39(4 Suppl):37-43. (PMID: 22500135)
Nat Cell Biol. 2017 Jul;19(7):856-863. (PMID: 28628083)
Front Microbiol. 2020 Jun 26;11:1297. (PMID: 32670231)
Mol Microbiol. 1992 Feb;6(3):283-91. (PMID: 1552844)
Viruses. 2019 May 16;11(5):. (PMID: 31100912)
Curr Biol. 2017 Mar 20;27(6):795-806. (PMID: 28262484)
Sci Rep. 2020 Jan 27;10(1):1218. (PMID: 31988337)
فهرسة مساهمة: Keywords: CHIKV; RNA helicases; apoptosis; label-free quantification; mass spectrometry; mitochondrion; mosquito cell culture; protein synthesis
المشرفين على المادة: 0 (Proteome)
تواريخ الأحداث: Date Created: 20220705 Date Completed: 20220707 Latest Revision: 20220817
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC9240781
DOI: 10.3389/fcimb.2022.920425
PMID: 35782121
قاعدة البيانات: MEDLINE
الوصف
تدمد:2235-2988
DOI:10.3389/fcimb.2022.920425