دورية أكاديمية
أعرض في EDS

Validation of a colorimetric LAMP to detect Loxosceles experimental envenomation.

التفاصيل البيبلوغرافية
العنوان: Validation of a colorimetric LAMP to detect Loxosceles experimental envenomation.
المؤلفون: Fernandes LP; Laboratory of Synthetic Biology and Biomimetics, Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil., Rocha MN; Laboratory of Synthetic Biology and Biomimetics, Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil., Duarte CG; Centro de Pesquisa e Desenvolvimento, Fundação Ezequiel Dias, 30510-010, Belo Horizonte, MG, Brazil., Minozzo JC; Production and Research Centre of Immunobiological Products, Department of Health of the State of Paraná, Piraquara 83302-200, Brazil., do Monte-Neto RL; Biotechnology Applied to Patogens (BAP) - Instituto René Rachou - Fundação Oswaldo Cruz, Belo Horizonte, 30190-009, Minas Gerais, Brazil., Felicori LF; Laboratory of Synthetic Biology and Biomimetics, Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil. Electronic address: liza@icb.ufmg.br.
المصدر: Toxicon : official journal of the International Society on Toxinology [Toxicon] 2022 Sep; Vol. 216, pp. 50-56. Date of Electronic Publication: 2022 Jul 01.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Pergamon Press Country of Publication: England NLM ID: 1307333 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3150 (Electronic) Linking ISSN: 00410101 NLM ISO Abbreviation: Toxicon Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Oxford ; New York : Pergamon Press, c1962-
مواضيع طبية MeSH: Spider Venoms*/genetics , Spider Venoms*/toxicity , Spiders*/genetics, Animals ; Colorimetry ; Molecular Diagnostic Techniques ; Nucleic Acid Amplification Techniques ; Phosphoric Diester Hydrolases/genetics ; Rabbits ; Sensitivity and Specificity
مستخلص: Diagnostic tests for brown spider accidents are unavailable and impact treatment decisions, increasing costs and patient risks. In this work, we used for the first time a fast, simple, and visual method based on the loop-mediated isothermal amplification assay (LAMP) to detect Loxosceles envenomation. Using the DNA from L. similis legs, we observed a high sensitivity using this test since as low as 0.32 pg of DNA could be detected. This pH-dependent colorimetric assay was 64 times more sensitive than PCR to detect spider DNA. The test was specific for Loxosceles once no cross-reaction was observed when testing DNA from different agents that cause similar dermonecrotic injuries. The test allowed the detection of Loxosceles intermedia DNA from hair, serum, and exudate samples obtained from experimentally-envenomed rabbit within 72 h. The method sensitivity varied according to the sample and the collection time, reaching 100% sensitivity in serum and hair, respectively, 1 h and 24 h after the experimental envenomation. Due to its ease of execution, speed, sensitivity, and specificity, LAMP presents an excellent potential for identifying Loxosceles spp. Envenomation. This can reduce the burden on the Health System and the morbidity for the patient by implementing the appropriate therapy immediately.In addition, this work opens up the perspective to other venomous animal accident identification using LAMP.
(Copyright © 2022 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: Envenomation; Isothermal DNA amplification; LAMP; Loxosceles DNA detection; Loxoscelism diagnosis
المشرفين على المادة: 0 (Spider Venoms)
EC 3.1.4.- (Phosphoric Diester Hydrolases)
SCR Protocol: LAMP assay
تواريخ الأحداث: Date Created: 20220705 Date Completed: 20220816 Latest Revision: 20220816
رمز التحديث: 20231215
DOI: 10.1016/j.toxicon.2022.06.017
PMID: 35787893
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-3150
DOI:10.1016/j.toxicon.2022.06.017