دورية أكاديمية
A Potential Role of Cholinergic Dysfunction on Impaired Colon Motility in Experimental Intestinal Chagas Disease.
| العنوان: | A Potential Role of Cholinergic Dysfunction on Impaired Colon Motility in Experimental Intestinal Chagas Disease. |
|---|---|
| المؤلفون: | Ricci MF; Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brasil., Béla SR; Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brasil.; Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brasil., Barbosa JL; Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brasil., Moraes MM; Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brasil., Mazzeti AL; Laboratório de Biologia Celular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brasil., Bahia MT; Escola de Medicina & Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brasil., Horta LS; Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brasil., Santiago HDC; Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brasil., Cruz JS; Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brasil., Capettini LDSA; Departamento de Farmacologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brasil., Arantes RME; Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brasil. |
| المصدر: | Journal of neurogastroenterology and motility [J Neurogastroenterol Motil] 2022 Jul 30; Vol. 28 (3), pp. 483-500. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: The Korean Society of Neurogastroenterology and Motility Country of Publication: Korea (South) NLM ID: 101530189 Publication Model: Print Cited Medium: Print ISSN: 2093-0879 (Print) Linking ISSN: 20930879 NLM ISO Abbreviation: J Neurogastroenterol Motil Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Seoul : The Korean Society of Neurogastroenterology and Motility |
| مستخلص: | Background/aims: Chagasic megacolon is caused by Trypanosoma cruzi , which promotes in several cases, irreversible segmental colonic dilation. This alteration is the major anatomic-clinical disorder, characterized by the enteric nervous system and muscle wall structural damage. Herein, we investigate how T. cruzi -induced progressive colonic structural changes modulate the colonic contractile pattern activity. Methods: We developed a murine model of T. cruzi -infection that reproduced long-term modifications of the enlarged colon. We evaluated colonic and total intestinal transit time in animals. The patterns of motor response at several time intervals between the acute and chronic phases were evaluated using the organ bath assays. Enteric motor neurons were stimulated by electric field stimulation. The responses were analyzed in the presence of the nicotinic and muscarinic acetylcholine receptor antagonists. Western blot was performed to evaluate the expression of nicotinic and muscarinic receptors. The neurotransmitter expression was analyzed by real-time polymerase chain reaction. Results: In the chronic phase of infection, there was decreased intestinal motility associated with decreased amplitude and rhythmicity of intestinal contractility. Pharmacological tests suggested a defective response mediated by acetylcholine receptors. The contractile response induced by acetylcholine was decreased by atropine in the acute phase while the lack of its action in the chronic phase was associated with tissue damage, and decreased expression of choline acetyltransferase, nicotinic subunits of acetylcholine receptors, and neurotransmitters. Conclusions: T. cruzi -induced damage of smooth muscles was accompanied by motility disorders such as decreased intestinal peristalsis and cholinergic system response impairment. This study allows integration of the natural history of Chagasic megacolon motility disorders and opens new perspectives for the design of effective therapeutic. |
| References: | Cell Tissue Res. 1997 Oct;290(1):61-9. (PMID: 9377643) Braz J Med Biol Res. 1985;18(3):255-64. (PMID: 3939103) Neurochem Int. 1994 Jul;25(1):93-7. (PMID: 7950977) Nat Rev Gastroenterol Hepatol. 2020 Jun;17(6):338-351. (PMID: 32152479) Eur Rev Med Pharmacol Sci. 2008 Aug;12 Suppl 1:3-19. (PMID: 18924440) Arq Gastroenterol. 1982 Jul-Sep;19(3):127-32. (PMID: 6821025) Gastroenterology. 2007 May;132(5):1912-24. (PMID: 17484884) Gastroenterology Res. 2017 Aug;10(4):213-217. (PMID: 28912906) Sci Rep. 2015 Jul 10;5:12095. (PMID: 26160593) Dig Dis Sci. 2005 Jul;50(7):1335-41. (PMID: 16047483) PLoS One. 2016 Apr 05;11(4):e0153038. (PMID: 27045678) Neurogastroenterol Motil. 2004 Apr;16 Suppl 1:34-8. (PMID: 15066002) Behav Brain Res. 2011 Aug 10;221(2):555-63. (PMID: 21145918) Am J Trop Med Hyg. 1987 Jan;36(1):41-5. (PMID: 3101527) Am J Physiol Gastrointest Liver Physiol. 2006 Oct;291(4):G584-94. (PMID: 16751171) Gastroenterology. 2007 Jul;133(1):175-83. (PMID: 17631141) Gastroenterology. 1993 Mar;104(3):690-7. (PMID: 8095033) Am J Gastroenterol. 1998 Jun;93(6):884-9. (PMID: 9647012) Infect Immun. 2004 Aug;72(8):4918-23. (PMID: 15271961) Hum Pathol. 2007 Aug;38(8):1256-64. (PMID: 17490721) Histochem Cell Biol. 2011 Jan;135(1):47-57. (PMID: 21184236) Gut. 1995 May;36(5):718-23. (PMID: 7541008) Peptides. 1988 Nov-Dec;9(6):1207-19. (PMID: 2470062) Am J Physiol. 1992 Jun;262(6 Pt 1):G1041-6. (PMID: 1616034) Front Cell Infect Microbiol. 2020 Oct 15;10:583899. (PMID: 33178632) Arq Gastroenterol. 2008 Apr-Jun;45(2):128-31. (PMID: 18622466) Res Commun Chem Pathol Pharmacol. 1988 May;60(2):261-4. (PMID: 3393734) Mol Biochem Parasitol. 2003 Jun;129(1):53-9. (PMID: 12798506) Br J Pharmacol. 2008 Dec;155(7):1043-55. (PMID: 18846038) J Pediatr Surg. 2006 May;41(5):1029-35. (PMID: 16677906) Adv Exp Med Biol. 2014;817:39-71. (PMID: 24997029) Hospital (Rio J). 1960 Jun;57:1057-62. (PMID: 14410284) JAMA. 2007 Nov 14;298(18):2171-81. (PMID: 18000201) Virchows Arch. 2001 Mar;438(3):254-8. (PMID: 11315622) Br J Pharmacol. 2010 Aug;160(7):1577-9. (PMID: 20649561) Br J Pharmacol. 2015 May;172(10):2427-32. (PMID: 25899710) Am J Pathol. 2004 Apr;164(4):1361-8. (PMID: 15039223) Auton Neurosci. 2006 Jun 30;126-127:232-49. (PMID: 16616700) CNS Neurol Disord Drug Targets. 2014;13(7):1294-303. (PMID: 25230223) Lancet. 1993 Aug 7;342(8867):338-40. (PMID: 7687730) Neurogastroenterol Motil. 2009 Dec;21(12):1326-e128. (PMID: 19650775) Int J Colorectal Dis. 2012 Sep;27(9):1181-9. (PMID: 22729712) Rev Soc Bras Med Trop. 1989 Oct-Dec;22(4):211-5. (PMID: 2518668) Adv Parasitol. 1968;6:63-116. (PMID: 4239747) Curr Opin Pharmacol. 2008 Aug;8(4):490-5. (PMID: 18675937) Naunyn Schmiedebergs Arch Pharmacol. 2020 Feb;393(2):243-251. (PMID: 31511953) Am J Trop Med Hyg. 2015 May;92(5):898-902. (PMID: 25778503) Rev Inst Med Trop Sao Paulo. 1962 Nov-Dec;4:389-96. (PMID: 14015230) J Pharmacol Exp Ther. 2006 Mar;316(3):1238-48. (PMID: 16303917) Br J Pharmacol. 2006 May;148(2):191-9. (PMID: 16520743) Dig Dis Sci. 1999 Jul;44(7):1265-72. (PMID: 10489904) Am J Physiol. 1994 Jan;266(1 Pt 1):G123-31. (PMID: 8304451) Am J Physiol Heart Circ Physiol. 2008 Dec;295(6):H2503-11. (PMID: 18952716) Am J Physiol Gastrointest Liver Physiol. 2021 Apr 1;320(4):G675-G687. (PMID: 33624530) Invest Clin. 2001 Sep;42(3):195-209. (PMID: 11552508) J Neurosci. 2014 Jul 30;34(31):10361-78. (PMID: 25080596) Br J Pharmacol. 2006 Dec;149(8):1022-30. (PMID: 17099717) J Neurosci. 2015 Aug 19;35(33):11543-58. (PMID: 26290232) Gastroenterology. 1993 Oct;105(4):1009-16. (PMID: 8104837) Wien Klin Wochenschr. 1956 Apr 27;68(17):333-9. (PMID: 13338284) Neurogastroenterol Motil. 2011 Nov;23(11):980-8. (PMID: 21895878) Gastroenterology. 2018 Aug;155(2):514-528.e6. (PMID: 29782847) |
| فهرسة مساهمة: | Keywords: Acetylcholine; Enteric nervous system; Gastrointestinal motility; Megacolon; Trypanosoma cruzi |
| تواريخ الأحداث: | Date Created: 20220707 Latest Revision: 20220731 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC9274474 |
| DOI: | 10.5056/jnm21074 |
| PMID: | 35799242 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2093-0879 |
|---|---|
| DOI: | 10.5056/jnm21074 |