دورية أكاديمية
أعرض في EDS

Proteomics and immunoblotting analyses reveal antigens that optimize the immunodiagnosis of the infection by Toxocara spp.

التفاصيل البيبلوغرافية
العنوان: Proteomics and immunoblotting analyses reveal antigens that optimize the immunodiagnosis of the infection by Toxocara spp.
المؤلفون: da Silva MB; Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Bahia, Brazil., Fernandes AMS; Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Bahia, Brazil., da Silva ES; Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Bahia, Brazil., Urrego JR; Department of Pharmaceutical Sciences, University of Cartagena, Cartagena, Colombia., Santiago LF; Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Bahia, Brazil., Garcés LFS; Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Bahia, Brazil., Portela RD; Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Bahia, Brazil., Pacheco LGC; Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Bahia, Brazil., Briza P; Department of Biosciences, University of Salzburg, Salzburg, Austria., Ferreira F; Department of Biosciences, University of Salzburg, Salzburg, Austria., Pinheiro CS; Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Bahia, Brazil., Alcantara-Neves NM; Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Bahia, Brazil.
المصدر: Transboundary and emerging diseases [Transbound Emerg Dis] 2022 Sep; Vol. 69 (5), pp. e2994-e3006. Date of Electronic Publication: 2022 Jul 19.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Blackwell Verlag Country of Publication: Germany NLM ID: 101319538 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1865-1682 (Electronic) Linking ISSN: 18651674 NLM ISO Abbreviation: Transbound Emerg Dis Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Berlin : Blackwell Verlag
مواضيع طبية MeSH: Toxocara canis* , Toxocariasis*/diagnosis, Animals ; Antigens, Helminth ; Enzyme-Linked Immunosorbent Assay/methods ; Enzyme-Linked Immunosorbent Assay/veterinary ; Humans ; Immunoblotting/veterinary ; Immunoglobulin G ; Immunologic Tests/veterinary ; Proteomics ; Recombinant Proteins ; Toxocara
مستخلص: Toxocariasis is an infection caused by the round worms Toxocara canis and Toxocara cati. It occurs worldwide though it is more prevalent in developing countries. For the diagnosis of toxocariasis, the most used method is the indirect enzyme-linked immunosorbent assay (indirect ELISA), based on the detection of specific antibodies using the excreted/secreted products from T. canis larvae (TES) as antigens, but it cross-reacts with several helminth infections. For this reason, there is a need to investigate species-specific immunoreactive proteins, which can be used for the development of a more sensitive and specific diagnosis. This study aims to investigate immunoreactive protein candidates to be used for the development of a more sensitive and specific diagnosis of Toxocara spp. infection in humans. We have used immunoblotting and mass spectrometry to select four Toxocara canis immunoreactive proteins that were recombinantly expressed in bacteria and evaluated as potential new diagnostic antigens (rMUC3, rTES 26, rTES32 and rCTL4). The recognition of these recombinant proteins by total serum IgG and IgG4 was assayed using the purified proteins in an isolated manner or in combination. The IgG ELISAs performed with individual recombinant antigens reached values of sensitivity and specificity that ranged from 91.7% to 97.3% and 94.0% to 97.9%, respectively. Among the analyses, the IgG4 immunoassay was proven to be more effective, revealing a sensitivity that ranged from 88.8% to 98.3% and a specificity of 97.8%-97.9%. The IgG4 ELISA was shown to be more effective and presented no cross-reactivity when using combinations of the rTES 26 and rCTL4 recombinant proteins. The combination of these two molecules achieved 100% sensitivity and specificity. The use of only two recombinant proteins can contribute to improve the current panorama of toxocariasis immunodiagnosis for, with a better optimization and reduced cost.
(© 2022 Wiley-VCH GmbH.)
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فهرسة مساهمة: Keywords: immunodiagnostic; immunoproteomics; recombinant proteins; toxocariasis
المشرفين على المادة: 0 (Antigens, Helminth)
0 (Immunoglobulin G)
0 (Recombinant Proteins)
تواريخ الأحداث: Date Created: 20220708 Date Completed: 20220929 Latest Revision: 20220929
رمز التحديث: 20240628
DOI: 10.1111/tbed.14650
PMID: 35801561
قاعدة البيانات: MEDLINE
الوصف
تدمد:1865-1682
DOI:10.1111/tbed.14650