دورية أكاديمية
Emergence of circulating influenza A H3N2 viruses with genetic drift in the matrix gene: be alert of false-negative test results.
العنوان: | Emergence of circulating influenza A H3N2 viruses with genetic drift in the matrix gene: be alert of false-negative test results. |
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المؤلفون: | Jørgensen RL; Department of Clinical Microbiology, Copenhagen University Hospital, Hvidovre Hospital, Hvidovre, Denmark., Lerche CJ; Department of Clinical Microbiology, Copenhagen University Hospital, Hvidovre Hospital, Hvidovre, Denmark., Pedersen MS; Department of Clinical Microbiology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark., Kirkby NS; Department of Clinical Microbiology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark., Botnen AB; National Influenza Center, Statens Serum Institut, Copenhagen, Denmark., Trebbien R; National Influenza Center, Statens Serum Institut, Copenhagen, Denmark., Nilsson-Møller S; Department of Clinical Microbiology, Copenhagen University Hospital, Hvidovre Hospital, Hvidovre, Denmark., Pinholt M; Department of Clinical Microbiology, Copenhagen University Hospital, Hvidovre Hospital, Hvidovre, Denmark., Nielsen ACY; Department of Clinical Microbiology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark., Westh H; Department of Clinical Microbiology, Copenhagen University Hospital, Hvidovre Hospital, Hvidovre, Denmark.; Institute of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark., Lisby JG; Department of Clinical Microbiology, Copenhagen University Hospital, Hvidovre Hospital, Hvidovre, Denmark., Schneider UV; Department of Clinical Microbiology, Copenhagen University Hospital, Hvidovre Hospital, Hvidovre, Denmark. |
المصدر: | APMIS : acta pathologica, microbiologica, et immunologica Scandinavica [APMIS] 2022 Oct; Vol. 130 (10), pp. 612-617. Date of Electronic Publication: 2022 Aug 10. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Munksgaard Country of Publication: Denmark NLM ID: 8803400 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1600-0463 (Electronic) Linking ISSN: 09034641 NLM ISO Abbreviation: APMIS Subsets: MEDLINE |
أسماء مطبوعة: | Publication: Copenhagen : Munksgaard Original Publication: Copenhagen : Munksgaard, c1988- |
مواضيع طبية MeSH: | Influenza A Virus, H1N1 Subtype*/genetics , Influenza A virus*/genetics , Influenza, Human*/diagnosis, Genetic Drift ; Humans ; Influenza A Virus, H3N2 Subtype/genetics |
مستخلص: | In March 2022, we observed samples with a negative fluorescent signal (60.5%, n = 43) for the influenza A matrix gene and a stronger positive signal for subtype A(H3N2). Forty-three samples were positive in InfA (H3N2) (mean Cq 30.9, range 23.9-35.1), and 26 of the 43 samples were negative in InfA matrix (mean Cq 28.0, range 23.2-30.6). Our multiplex test is a laboratory-developed four-target, four-color influenza A reverse-transcription PCR assay targeting the matrix gene, subtypes A(H3N2) and A(H1N1)pdm09. Several samples were negative when retested on commercial influenza Point-of-Care assays. As the matrix gene is a stand-alone target in most commercial diagnostic assays, we caution against false-negative subtype A test results. (© 2022 The Authors. APMIS published by John Wiley & Sons Ltd on behalf of Scandinavian Societies for Medical Microbiology and Pathology.) |
References: | Euro Surveill. 2020 Nov;25(44):. (PMID: 33153522) Science. 2006 Apr 21;312(5772):384-8. (PMID: 16627734) Proc Natl Acad Sci U S A. 1981 Jul;78(7):4170-4. (PMID: 6945577) Virol J. 2009 May 29;6:67. (PMID: 19476650) Ann Intern Med. 2021 Nov;174(11):ITC161-ITC176. (PMID: 34748378) Euro Surveill. 2022 Apr;27(15):. (PMID: 35426364) J Antimicrob Chemother. 2021 Sep 23;76(Suppl 3):iii28-iii32. (PMID: 34555156) Euro Surveill. 2022 Apr;27(15):. (PMID: 35426361) Euro Surveill. 2020 Nov;25(44):. (PMID: 33153518) J Virol. 1991 Oct;65(10):5491-8. (PMID: 1895397) |
فهرسة مساهمة: | Keywords: Assay; H3N2; M gene; RT-PCR; diagnostic; genetic drift; mutations; sequencing; surveillance |
تواريخ الأحداث: | Date Created: 20220715 Date Completed: 20220907 Latest Revision: 20221015 |
رمز التحديث: | 20231215 |
مُعرف محوري في PubMed: | PMC9544743 |
DOI: | 10.1111/apm.13262 |
PMID: | 35836366 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1600-0463 |
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DOI: | 10.1111/apm.13262 |