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Emergence of circulating influenza A H3N2 viruses with genetic drift in the matrix gene: be alert of false-negative test results.

التفاصيل البيبلوغرافية
العنوان: Emergence of circulating influenza A H3N2 viruses with genetic drift in the matrix gene: be alert of false-negative test results.
المؤلفون: Jørgensen RL; Department of Clinical Microbiology, Copenhagen University Hospital, Hvidovre Hospital, Hvidovre, Denmark., Lerche CJ; Department of Clinical Microbiology, Copenhagen University Hospital, Hvidovre Hospital, Hvidovre, Denmark., Pedersen MS; Department of Clinical Microbiology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark., Kirkby NS; Department of Clinical Microbiology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark., Botnen AB; National Influenza Center, Statens Serum Institut, Copenhagen, Denmark., Trebbien R; National Influenza Center, Statens Serum Institut, Copenhagen, Denmark., Nilsson-Møller S; Department of Clinical Microbiology, Copenhagen University Hospital, Hvidovre Hospital, Hvidovre, Denmark., Pinholt M; Department of Clinical Microbiology, Copenhagen University Hospital, Hvidovre Hospital, Hvidovre, Denmark., Nielsen ACY; Department of Clinical Microbiology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark., Westh H; Department of Clinical Microbiology, Copenhagen University Hospital, Hvidovre Hospital, Hvidovre, Denmark.; Institute of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark., Lisby JG; Department of Clinical Microbiology, Copenhagen University Hospital, Hvidovre Hospital, Hvidovre, Denmark., Schneider UV; Department of Clinical Microbiology, Copenhagen University Hospital, Hvidovre Hospital, Hvidovre, Denmark.
المصدر: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica [APMIS] 2022 Oct; Vol. 130 (10), pp. 612-617. Date of Electronic Publication: 2022 Aug 10.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Munksgaard Country of Publication: Denmark NLM ID: 8803400 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1600-0463 (Electronic) Linking ISSN: 09034641 NLM ISO Abbreviation: APMIS Subsets: MEDLINE
أسماء مطبوعة: Publication: Copenhagen : Munksgaard
Original Publication: Copenhagen : Munksgaard, c1988-
مواضيع طبية MeSH: Influenza A Virus, H1N1 Subtype*/genetics , Influenza A virus*/genetics , Influenza, Human*/diagnosis, Genetic Drift ; Humans ; Influenza A Virus, H3N2 Subtype/genetics
مستخلص: In March 2022, we observed samples with a negative fluorescent signal (60.5%, n = 43) for the influenza A matrix gene and a stronger positive signal for subtype A(H3N2). Forty-three samples were positive in InfA (H3N2) (mean Cq 30.9, range 23.9-35.1), and 26 of the 43 samples were negative in InfA matrix (mean Cq 28.0, range 23.2-30.6). Our multiplex test is a laboratory-developed four-target, four-color influenza A reverse-transcription PCR assay targeting the matrix gene, subtypes A(H3N2) and A(H1N1)pdm09. Several samples were negative when retested on commercial influenza Point-of-Care assays. As the matrix gene is a stand-alone target in most commercial diagnostic assays, we caution against false-negative subtype A test results.
(© 2022 The Authors. APMIS published by John Wiley & Sons Ltd on behalf of Scandinavian Societies for Medical Microbiology and Pathology.)
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فهرسة مساهمة: Keywords: Assay; H3N2; M gene; RT-PCR; diagnostic; genetic drift; mutations; sequencing; surveillance
تواريخ الأحداث: Date Created: 20220715 Date Completed: 20220907 Latest Revision: 20221015
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC9544743
DOI: 10.1111/apm.13262
PMID: 35836366
قاعدة البيانات: MEDLINE
الوصف
تدمد:1600-0463
DOI:10.1111/apm.13262