دورية أكاديمية
Raman Spectroscopy Enables Confirmatory Diagnostics of Fusarium Wilt in Asymptomatic Banana.
| العنوان: | Raman Spectroscopy Enables Confirmatory Diagnostics of Fusarium Wilt in Asymptomatic Banana. |
|---|---|
| المؤلفون: | Parlamas S; Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX, United States., Goetze PK; Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX, United States., Humpal D; Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX, United States., Kurouski D; Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX, United States.; Institute for Advancing Health Through Agriculture, Texas A&M University, College Station, TX, United States., Jo YK; Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX, United States. |
| المصدر: | Frontiers in plant science [Front Plant Sci] 2022 Jun 15; Vol. 13, pp. 922254. Date of Electronic Publication: 2022 Jun 15 (Print Publication: 2022). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101568200 Publication Model: eCollection Cited Medium: Print ISSN: 1664-462X (Print) Linking ISSN: 1664462X NLM ISO Abbreviation: Front Plant Sci Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Research Foundation, 2010- |
| مستخلص: | Fusarium oxysporum f. sp. cubense (FOC) causes Fusarium wilt, one of the most concerning diseases in banana ( Musa spp.), compromising global banana production. There are limited curative management options after FOC infections, and early Fusarium wilt symptoms are similar with other abiotic stress factors such as drought. Therefore, finding a reliable and timely form of early detection and proper diagnostics is critical for disease management for FOC. In this study, Portable Raman spectroscopy (handheld Raman spectrometer equipped with 830 nm laser source) was applied for developing a confirmatory diagnostic tool for early infection of FOC on asymptomatic banana. Banana plantlets were inoculated with FOC; uninoculated plants exposed to a drier condition were also prepared compared to well-watered uninoculated control plants. Subsequent Raman readings from the plant leaves, without damaging or destroying them, were performed weekly. The conditions of biotic and abiotic stresses on banana were modeled to examine and identify specific Raman spectra suitable for diagnosing FOC infection. Our results showed that Raman spectroscopy could be used to make highly accurate diagnostics of FOC at the asymptomatic stage. Based on specific Raman spectra at vibrational bands 1,155, 1,184, and 1,525 cm -1 , Raman spectroscopy demonstrated nearly 100% accuracy of FOC diagnosis at 40 days after inoculation, differentiating FOC-infected plants from uninoculated plants that were well-watered or exposed to water deficit condition. This study first reported that Raman spectroscopy can be used as a rapid and non-destructive tool for banana Fusarium wilt diagnostics. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Parlamas, Goetze, Humpal, Kurouski and Jo.) |
| References: | Front Plant Sci. 2019 Aug 06;10:1006. (PMID: 31447871) Phytopathology. 2006 Jun;96(6):653-6. (PMID: 18943184) Planta. 2019 Oct;250(4):1247-1254. (PMID: 31222494) Phys Chem Chem Phys. 2009 Dec 28;11(48):11471-8. (PMID: 20024418) Planta. 2020 Feb 11;251(3):64. (PMID: 32048047) PLoS One. 2020 Mar 16;15(3):e0230330. (PMID: 32176731) Spectrochim Acta A Mol Biomol Spectrosc. 1997 Nov;53A(13):2383-92. (PMID: 9477578) Forensic Sci Int. 2009 Jul 1;188(1-3):1-17. (PMID: 19328638) Analyst. 2015 Aug 7;140(15):4967-80. (PMID: 26042229) Anal Bioanal Chem. 2010 Aug;397(7):2693-701. (PMID: 20213166) Nat Methods. 2019 Dec;16(12):1199-1200. (PMID: 31780825) Proc Natl Acad Sci U S A. 2017 Mar 28;114(13):3393-3396. (PMID: 28289201) Anal Biochem. 2017 Sep 1;532:60-63. (PMID: 28602750) Annu Rev Phytopathol. 2003;41:305-24. (PMID: 14527331) Planta. 2006 Oct;224(5):1141-53. (PMID: 16761135) FEBS Lett. 2014 May 2;588(9):1637-43. (PMID: 24631541) Anal Chem. 2018 Mar 6;90(5):3009-3012. (PMID: 29461798) Appl Spectrosc. 2007 Jan;61(1):32-7. (PMID: 17311714) Front Plant Sci. 2020 Oct 22;11:573321. (PMID: 33193509) Anal Bioanal Chem. 2019 May;411(14):3125-3133. (PMID: 30989272) ACS Chem Neurosci. 2018 Mar 21;9(3):404-420. (PMID: 29308873) Sci Rep. 2020 Nov 19;10(1):20206. (PMID: 33214575) Plant J. 2017 Jun;90(6):1187-1195. (PMID: 28301688) Sci Rep. 2020 Jun 22;10(1):10101. (PMID: 32572139) Mol Plant Pathol. 2012 May;13(4):414-30. (PMID: 22471698) Front Plant Sci. 2020 Sep 03;11:01300. (PMID: 33013951) Anal Chem. 2018 Jul 17;90(14):8616-8621. (PMID: 29898358) Front Plant Sci. 2021 Jan 20;11:616672. (PMID: 33552109) Anal Chem. 2019 Jul 16;91(14):9025-9031. (PMID: 31265250) |
| فهرسة مساهمة: | Keywords: Fusarium oxysporum f. sp. cubense; Fusarium wilt; Raman spectroscopy; banana; diagnostics |
| تواريخ الأحداث: | Date Created: 20220715 Latest Revision: 20220716 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC9275401 |
| DOI: | 10.3389/fpls.2022.922254 |
| PMID: | 35837469 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1664-462X |
|---|---|
| DOI: | 10.3389/fpls.2022.922254 |