دورية أكاديمية
أعرض في EDS

Functional changes in hemostasis during asexual and sexual parasitemia in a controlled human malaria infection.

التفاصيل البيبلوغرافية
العنوان: Functional changes in hemostasis during asexual and sexual parasitemia in a controlled human malaria infection.
المؤلفون: Huang S; Department of Functional Coagulation, Synapse Research Institute, Maastricht, The Netherlands.; Department of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands., van der Heijden W; Department of Internal Medicine, Radboud Center for Infectious Diseases, Radboud University Medical Centre, Nijmegen, The Netherlands., Reuling IJ; Department of Medical Microbiology, Radboud University Medical Centre, Nijmegen, The Netherlands., Wan J; Department of Functional Coagulation, Synapse Research Institute, Maastricht, The Netherlands.; Department of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands., Yan Q; Department of Functional Coagulation, Synapse Research Institute, Maastricht, The Netherlands.; Department of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands., de Laat-Kremers RMW; Department of Data Analysis and Artificial Intelligence, Synapse Research Institute, Maastricht, The Netherlands., Van der Ven AJ; Department of Internal Medicine, Radboud Center for Infectious Diseases, Radboud University Medical Centre, Nijmegen, The Netherlands., de Groot PG; Department of Functional Coagulation, Synapse Research Institute, Maastricht, The Netherlands., McCall M; Department of Medical Microbiology, Radboud University Medical Centre, Nijmegen, The Netherlands., Sauerwein RW; Department of Medical Microbiology, Radboud University Medical Centre, Nijmegen, The Netherlands., Bousema T; Department of Medical Microbiology, Radboud University Medical Centre, Nijmegen, The Netherlands., Roest M; Department of Platelet pathophysiology, Synapse Research Institute, Maastricht, The Netherlands., Ninivaggi M; Department of Functional Coagulation, Synapse Research Institute, Maastricht, The Netherlands., de Mast Q; Department of Internal Medicine, Radboud Center for Infectious Diseases, Radboud University Medical Centre, Nijmegen, The Netherlands., de Laat B; Department of Functional Coagulation, Synapse Research Institute, Maastricht, The Netherlands.; Department of Data Analysis and Artificial Intelligence, Synapse Research Institute, Maastricht, The Netherlands.
المصدر: PloS one [PLoS One] 2022 Jul 15; Vol. 17 (7), pp. e0271527. Date of Electronic Publication: 2022 Jul 15 (Print Publication: 2022).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Hemostasis*/physiology , Malaria*/complications , Malaria*/metabolism , Parasitemia*/complications , Parasitemia*/metabolism, Blood Platelets/metabolism ; Humans ; Prothrombin/metabolism ; Thrombin/metabolism ; von Willebrand Factor/metabolism
مستخلص: Decreased platelet count is an early phenomenon in asexual Plasmodium falciparum parasitemia, but its association with acute or long-term functional changes in platelets and coagulation is unknown. Moreover, the impact of gametocytemia on platelets and coagulation remains unclear. We investigated the changes in platelet number and function during early asexual parasitemia, gametocytemia and convalescence in 16 individuals participating in a controlled human malaria infection study, and studied its relationship with changes in total and active von Willebrand factor levels (VWF) and the coagulation system. Platelet activation and reactivity were determined by flow cytometry, and the coagulation system was assessed using different representative assays including antigen assays, activity assays and global functional assays. Platelet count was decreased during asexual blood stage infection but normalized during gametocytemia. Platelet P-selectin expression was slightly increased during asexual parasitemia, gametocytemia and at day 64. In contrast, platelet reactivity to different agonists remained unchanged, except a marked decrease in reactivity to low dose collagen-related peptide-XL. Thrombin generation and antigen assays did not show a clear activation of the coagulation during asexual parasitemia, whereas total and active VWF levels were markedly increased. During gametocytemia and on day 64, the endogenous thrombin potential, thrombin peak and velocity index were increased and prothrombin conversion and plasma prothrombin levels were decreased. We conclude that the decreased platelet count during asexual parasitemia is associated with increased active VWF levels (i.e. endothelial activation), but not platelet hyperreactivity or hypercoagulability, and that the increased platelet clearance in asexual parasitemia could cause spontaneous VWF-platelet complexes formation.
Competing Interests: The authors have declared that no competing interests exist.
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المشرفين على المادة: 0 (von Willebrand Factor)
9001-26-7 (Prothrombin)
EC 3.4.21.5 (Thrombin)
تواريخ الأحداث: Date Created: 20220715 Date Completed: 20220719 Latest Revision: 20220802
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC9286275
DOI: 10.1371/journal.pone.0271527
PMID: 35839244
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0271527