دورية أكاديمية
أعرض في EDS

Ascorbate oxidase enabling glucometer readout for portable detection of hydrogen peroxide.

التفاصيل البيبلوغرافية
العنوان: Ascorbate oxidase enabling glucometer readout for portable detection of hydrogen peroxide.
المؤلفون: Tian T; Chongqing Key Laboratory of High Active Traditional Chinese Drug Delivery System, Chongqing Medical and Pharmaceutical College, Chongqing 401331, China; School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China., Zhang H; Chongqing Key Laboratory of High Active Traditional Chinese Drug Delivery System, Chongqing Medical and Pharmaceutical College, Chongqing 401331, China. Electronic address: zhanghao199392@163.com., Yang FQ; School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China. Electronic address: fengqingyang@cqu.edu.cn.
المصدر: Enzyme and microbial technology [Enzyme Microb Technol] 2022 Oct; Vol. 160, pp. 110096. Date of Electronic Publication: 2022 Jul 09.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: United States NLM ID: 8003761 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0909 (Electronic) Linking ISSN: 01410229 NLM ISO Abbreviation: Enzyme Microb Technol Subsets: MEDLINE
أسماء مطبوعة: Publication: New York, NY : Elsevier
Original Publication: [Guildford, Eng.] IPC Science and Technology Press.
مواضيع طبية MeSH: Ascorbate Oxidase*/metabolism , Hydrogen Peroxide*/analysis, Ascorbic Acid ; Glucose ; Humans ; Limit of Detection ; Oxidation-Reduction
مستخلص: A rapid, portable, and cost-effective method using personal glucose meter (PGM) for quantitative analysis of hydrogen peroxide (H 2 O 2 ) was established based on ascorbate oxidase (AAO)-catalyzed reaction for the first time. Ascorbic acid (AA) can rapidly reduce ferricyanide (K 3 [Fe(CN) 6 ]) to ferrocyanide (K 4 [Fe(CN) 6 ]) in the glucose test strip and transfer electron to the electrode to generating a PGM detectable signal. Thus, the concentration of AA can be directly determined by the PGM as simple as measuring the blood glucose. On the other hand, AAO can catalyze the reduction of H 2 O 2 and produce an enzyme-peroxide complex, which decreases the yields of dehydroascorbic acid formed by the oxidation of AA, resulting in the increase in PGM detectable signal of residual ascorbic acid (re-AA). Therefore, the concentration of H 2 O 2 is proportional to the concentration of re-AA. After optimization of the experimental conditions, the developed method can be used to detect H 2 O 2 at linear range of 2.5-5 × 10 3 μM with the quantification limit of 2.5 μM. In addition, the satisfactory spiked recoveries (95.3-108.9 %) of real samples (i.e., tap water, contact lens solution, medical hydrogen peroxide, and normal human serum) confirm its feasibility for practical applications. In short, this study provides a feasible PGM-based method for H 2 O 2 detection with simple operations.
(Copyright © 2022 Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: Ascorbate oxidase; Ascorbic acid; Hydrogen peroxide; Personal glucose meter; Point-of-care testing
المشرفين على المادة: BBX060AN9V (Hydrogen Peroxide)
EC 1.10.3.3 (Ascorbate Oxidase)
IY9XDZ35W2 (Glucose)
PQ6CK8PD0R (Ascorbic Acid)
تواريخ الأحداث: Date Created: 20220715 Date Completed: 20220809 Latest Revision: 20220809
رمز التحديث: 20240628
DOI: 10.1016/j.enzmictec.2022.110096
PMID: 35839591
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0909
DOI:10.1016/j.enzmictec.2022.110096