دورية أكاديمية
أعرض في EDS

Platelet proteome reveals features of cell death, antiviral response and viral replication in covid-19.

التفاصيل البيبلوغرافية
العنوان: Platelet proteome reveals features of cell death, antiviral response and viral replication in covid-19.
المؤلفون: Trugilho MRO; Center for Technological Development in Health, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil. monique.trugilho@fiocruz.br.; Laboratory of Toxinology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil. monique.trugilho@fiocruz.br., Azevedo-Quintanilha IG; Laboratory of Immunopharmacology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil., Gesto JSM; Center for Technological Development in Health, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil., Moraes ECS; Laboratory of Toxinology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.; Laboratory of Immunopharmacology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil., Mandacaru SC; Center for Technological Development in Health, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil., Campos MM; Laboratory of Immunopharmacology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil., Oliveira DM; Laboratory of Immunopharmacology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil., Dias SSG; Laboratory of Immunopharmacology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil., Bastos VA; Laboratory of Toxinology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil., Santos MDM; Laboratory for Structural and Computational Proteomics, Carlos Chagas Institute, Oswaldo Cruz Foundation, Curitiba, Brazil., Carvalho PC; Laboratory for Structural and Computational Proteomics, Carlos Chagas Institute, Oswaldo Cruz Foundation, Curitiba, Brazil., Valente RH; Laboratory of Toxinology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil., Hottz ED; Laboratory of Immunothrombosis, Department of Biochemistry, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil., Bozza FA; National Institute of Infectious Disease Evandro Chagas, Oswaldo Cruz Foundation, and D'Or Institute for Research and Education, Rio de Janeiro, Brazil., Souza TML; Center for Technological Development in Health, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.; National Institute for Science and Technology on Innovation on Diseases of Neglected Populations, Rio de Janeiro, Brazil., Perales J; Laboratory of Toxinology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil., Bozza PT; Laboratory of Immunopharmacology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil. pbozza@ioc.fiocruz.br.
المصدر: Cell death discovery [Cell Death Discov] 2022 Jul 16; Vol. 8 (1), pp. 324. Date of Electronic Publication: 2022 Jul 16.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: United States NLM ID: 101665035 Publication Model: Electronic Cited Medium: Print ISSN: 2058-7716 (Print) Linking ISSN: 20587716 NLM ISO Abbreviation: Cell Death Discov Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: [New York, NY] : Nature Publishing Group, [2015]-
مستخلص: Coronavirus disease 2019 (COVID-19) has affected over 400 million people worldwide, leading to 6 million deaths. Among the complex symptomatology of COVID-19, hypercoagulation and thrombosis have been described to directly contribute to lethality, pointing out platelets as an important SARS-CoV-2 target. In this work, we explored the platelet proteome of COVID-19 patients through a label-free shotgun proteomics approach to identify platelet responses to infection, as well as validation experiments in a larger patient cohort. Exclusively detected proteins (EPs) and differentially expressed proteins (DEPs) were identified in the proteomic dataset and thus classified into biological processes to map pathways correlated with pathogenesis. Significant changes in the expression of proteins related to platelet activation, cell death, and antiviral response through interferon type-I were found in all patients. Since the outcome of COVID-19 varies highly among individuals, we also performed a cross-comparison of proteins found in survivors and nonsurvivors. Proteins belonging to the translation pathway were strongly highlighted in the nonsurvivor group. Moreover, the SARS-CoV-2 genome was fully sequenced in platelets from five patients, indicating viral internalization and preprocessing, with CD147 as a potential entry route. In summary, platelets play a significant role in COVID-19 pathogenesis via platelet activation, antiviral response, and disease severity.
(© 2022. The Author(s).)
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تواريخ الأحداث: Date Created: 20220716 Latest Revision: 20221115
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC9287722
DOI: 10.1038/s41420-022-01122-1
PMID: 35842415
قاعدة البيانات: MEDLINE
الوصف
تدمد:2058-7716
DOI:10.1038/s41420-022-01122-1