التفاصيل البيبلوغرافية
| العنوان: |
PCR-RLFP characterization of Leishmania spp. in domestic animals from the south-western border of Brazil. |
| المؤلفون: |
Pradella GD; Universidade Federal do Pampa - UNIPAMPA, Uruguaiana, RS, Brasil., Escobar TA; Universidade Federal do Pampa - UNIPAMPA, Uruguaiana, RS, Brasil., Santos TPD; Universidade Federal do Pampa - UNIPAMPA, Uruguaiana, RS, Brasil., Vargas RC; Universidade Federal do Pampa - UNIPAMPA, Uruguaiana, RS, Brasil., Góss GC; Universidade Federal do Pampa - UNIPAMPA, Uruguaiana, RS, Brasil., Ferrareze PAG; Universidade Federal de Ciências da Saúde de Porto Alegre - UFCSPA, Porto Alegre, RS, Brasil., Rosa E Silva LK; Universidade Federal do Rio Grande do Sul -- UFRGS, Porto Alegre, RS, Brasil., Zuravski L; Universidade Federal do Pampa - UNIPAMPA, Uruguaiana, RS, Brasil., Pereira KB; Universidade Federal do Pampa - UNIPAMPA, Uruguaiana, RS, Brasil., Duarte CA; Universidade Federal do Pampa - UNIPAMPA, Uruguaiana, RS, Brasil., Lübeck I; Universidade Federal do Pampa - UNIPAMPA, Uruguaiana, RS, Brasil. |
| المصدر: |
Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria [Rev Bras Parasitol Vet] 2022 Jul 18; Vol. 31 (3), pp. e005222. Date of Electronic Publication: 2022 Jul 18 (Print Publication: 2022). |
| نوع المنشور: |
Journal Article |
| اللغة: |
English |
| بيانات الدورية: |
Publisher: Colégio Brasileiro de Parasitologia Veterinária Country of Publication: Brazil NLM ID: 9440482 Publication Model: eCollection Cited Medium: Internet ISSN: 1984-2961 (Electronic) Linking ISSN: 0103846X NLM ISO Abbreviation: Rev Bras Parasitol Vet Subsets: MEDLINE |
| أسماء مطبوعة: |
Original Publication: São Paulo, Brasil : Colégio Brasileiro de Parasitologia Veterinária, [1992]- |
| مواضيع طبية MeSH: |
Cat Diseases*/diagnosis , Cat Diseases*/epidemiology , Cat Diseases*/parasitology , Dog Diseases*/diagnosis , Dog Diseases*/parasitology , Leishmania infantum*/genetics , Leishmaniasis, Visceral*/veterinary, Animals ; Animals, Domestic ; Brazil ; Cats ; DNA, Kinetoplast/genetics ; Dogs ; Phylogeny ; Polymerase Chain Reaction/veterinary |
| مستخلص: |
The aim of this study was to characterize Leishmania spp. from canine and feline samples using Polymerase Chain Reaction (PCR)- Restriction Fragment Length Polymorphism (RFLP). It was conducted in the southern region of Brazil, located at border crossings to Argentina and Uruguay. Samples were collected from 116 dogs (Canis lupus familiaris) and 89 cats (Felis catus). The PCR was performed to screen for an LT1 fragment from kinetoplast DNA (kDNA) target gene, and positive samples were subjected to a second PCR for an internal transcribed spacers (ITS1) region from ribosomal DNA (rDNA) target. RFLP was performed using the Haemophilus aegyptius (HAE III) restriction endonuclease (Fermentas ®). Positive samples by PCR ITS1 were sequenced and deposited in NCBI GenBank, and a phylogenetic analysis was developed. We found that 12.9% (15/116) of the samples from dogs were positive. All the 89 cat samples were negative. Positive samples were tested against Leishmania reference strains presenting different patterns in PCR-RFLP, and these samples showed bands denoting similarity to the standard species of Leishmania infantum, proven through sequencing and phylogenetic analysis. The RFLP technique, alone, was shown to be feasible for practical application and confirmation of the involved Leishmania spp. |
| المشرفين على المادة: |
0 (DNA, Kinetoplast) |
| تواريخ الأحداث: |
Date Created: 20220720 Date Completed: 20220722 Latest Revision: 20220722 |
| رمز التحديث: |
20240628 |
| DOI: |
10.1590/S1984-29612022035 |
| PMID: |
35858013 |
| قاعدة البيانات: |
MEDLINE |
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