دورية أكاديمية
أعرض في EDS

Activity of Delafloxacin and Levofloxacin against Stenotrophomonas maltophilia at Simulated Plasma and Intrapulmonary pH Values.

التفاصيل البيبلوغرافية
العنوان: Activity of Delafloxacin and Levofloxacin against Stenotrophomonas maltophilia at Simulated Plasma and Intrapulmonary pH Values.
المؤلفون: Vialichka A; College of Pharmacy, University of Illinois Chicago, Chicago, Illinois, USA., Biagi M; College of Pharmacy, University of Illinois Chicago, Rockford, Illinois, USA., Meyer K; College of Pharmacy, University of Illinois Chicago, Chicago, Illinois, USA., Wu T; College of Pharmacy, University of Illinois Chicago, Chicago, Illinois, USA., Shajee A; College of Pharmacy, University of Illinois Chicago, Chicago, Illinois, USA., Tan X; College of Pharmacy, University of Illinois Chicago, Chicago, Illinois, USA., Wenzler E; College of Pharmacy, University of Illinois Chicago, Chicago, Illinois, USA.
المصدر: Microbiology spectrum [Microbiol Spectr] 2022 Aug 31; Vol. 10 (4), pp. e0270521. Date of Electronic Publication: 2022 Jul 11.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: ASM Press Country of Publication: United States NLM ID: 101634614 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2165-0497 (Electronic) Linking ISSN: 21650497 NLM ISO Abbreviation: Microbiol Spectr Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, DC : ASM Press, 2013-
مواضيع طبية MeSH: Gram-Negative Bacterial Infections* , Stenotrophomonas maltophilia*, Anti-Bacterial Agents/pharmacology ; Fluoroquinolones/pharmacology ; Humans ; Hydrogen-Ion Concentration ; Levofloxacin/pharmacology ; Microbial Sensitivity Tests
مستخلص: Fluoroquinolones have become a popular treatment option for Stenotrophomonas maltophilia infections. Although levofloxacin is most commonly used, delafloxacin demonstrates comparable in vitro activity when evaluated under standard susceptibility testing conditions at neutral pH. At acidic pH, the activity of the anionic delafloxacin is improved, while the activity of the zwitterionic levofloxacin is reduced. Because the human respiratory tract has a pH of ~6.6 and is the most common site of S. maltophilia infection, it is vital to understand the activity of these agents in this environment. Therefore, levofloxacin and delafloxacin were tested against clinical S. maltophilia isolates via broth microdilution testing ( n  = 37) and time-kill analysis ( n  = 5) in neutral cation-adjusted Mueller-Hinton broth (CAMHB) (pH 7.3) and acidic CAMHB (aCAMHB) (pH 6.5). In CAMHB, MIC 50 values were similar between levofloxacin and delafloxacin (8 mg/L versus 8 mg/L). In aCAMHB, levofloxacin MICs did not change, while delafloxacin MICs decreased by a median of 4 log 2 dilutions (MIC 50 values of 8 mg/L versus 0.25 mg/L). In time-kill analyses, levofloxacin and delafloxacin at the maximum drug concentration for the free drug ( fC max ) were bactericidal against 3 and 2 isolates in CAMHB, respectively. In aCAMHB, levofloxacin was not bactericidal against any isolate, while delafloxacin was bactericidal against the same 2 isolates. Relative to CAMHB, levofloxacin activity was reduced by 2.5 log 10 CFU/mL in aCAMHB, whereas delafloxacin activity was increased 2.7 log 10 CFU/mL. Although the bactericidal activity of levofloxacin against S. maltophilia was attenuated in an acidic environment in this study, the increased potency of delafloxacin at pH 6.5 did not translate into improved bactericidal activity in time-kill analyses, compared to pH 7.3. IMPORTANCE Stenotrophomonas maltophilia most often infects the lungs, where the physiologic environment is naturally slightly acidic (pH ~6.6), compared to most parts of the body (such as the bloodstream), which have neutral pH values (~7.4). Pneumonia due to S. maltophilia is often treated with the antibiotic levofloxacin, despite the activity of levofloxacin being known to be impaired at acidic pH. Unfortunately, currently available methods for susceptibility testing of levofloxacin against S. maltophilia are performed at a neutral pH and therefore may not accurately represent the activity of levofloxacin at the site of infection in the lungs. A similar but newer antibiotic in the same class as levofloxacin, namely, delafloxacin, is not affected by being in an acidic environment and may actually work better at lower pH values. Therefore, the purpose of this study was to investigate whether one drug might be better than the other in this setting by testing each agent's ability to kill S. maltophilia at pH 7.3 and pH 6.5. These findings could then be used to design confirmatory studies that may ultimately impact which drug is given to patients with lung infections due to S. maltophilia.
References: J Antimicrob Chemother. 2003 Dec;52(6):925-30. (PMID: 14585864)
Pharmaceutics. 2020 Aug 14;12(8):. (PMID: 32823957)
Int J Antimicrob Agents. 2021 Aug;58(2):106367. (PMID: 34058337)
Antimicrob Agents Chemother. 2006 Mar;50(3):841-51. (PMID: 16495241)
Drugs Exp Clin Res. 1985;11(5):335-8. (PMID: 2941261)
Antimicrob Agents Chemother. 1996 Dec;40(12):2859-64. (PMID: 9124855)
Int J Mol Sci. 2021 Mar 25;22(7):. (PMID: 33806154)
Open Forum Infect Dis. 2022 Jan 17;9(2):ofab644. (PMID: 35097154)
J Antimicrob Chemother. 2008 Nov;62(5):889-94. (PMID: 18662945)
J Clin Microbiol. 2021 Aug 18;59(9):e0065421. (PMID: 34011524)
J Bacteriol. 2019 Mar 13;201(7):. (PMID: 30642989)
Lung. 2004;182(5):297-317. (PMID: 15742242)
J Antimicrob Chemother. 2021 Sep 15;76(10):2615-2621. (PMID: 34212183)
J Clin Microbiol. 2020 Jan 28;58(2):. (PMID: 31748318)
Future Microbiol. 2021 Jan;16(2):83-93. (PMID: 33470844)
Clin Infect Dis. 2001 May 15;32 Suppl 2:S104-13. (PMID: 11320451)
Open Forum Infect Dis. 2020 Apr 22;7(5):ofaa141. (PMID: 32462047)
Diagn Microbiol Infect Dis. 2005 Jan;51(1):39-43. (PMID: 15629227)
Antimicrob Agents Chemother. 2019 Oct 22;63(11):. (PMID: 31427295)
Antimicrob Agents Chemother. 2020 Jan 27;64(2):. (PMID: 31767717)
J Urol. 2015 Aug;194(2):563-70. (PMID: 25637857)
JAC Antimicrob Resist. 2022 May 05;4(3):dlac040. (PMID: 35529051)
Antimicrob Agents Chemother. 2011 Feb;55(2):649-58. (PMID: 21135179)
PLoS One. 2016 Mar 21;11(3):e0152132. (PMID: 26999818)
Clin Infect Dis. 2022 Jul 6;74(12):2089-2114. (PMID: 34864936)
PLoS One. 2016 Jun 03;11(6):e0156690. (PMID: 27257956)
Nat Commun. 2017 Aug 1;8(1):171. (PMID: 28761097)
JAMA. 1998 Jan 14;279(2):125-9. (PMID: 9440662)
J Antimicrob Chemother. 2020 Apr 1;75(4):997-1005. (PMID: 31930305)
Antimicrob Agents Chemother. 2020 Aug 20;64(9):. (PMID: 32571820)
Antimicrob Agents Chemother. 2018 Mar 27;62(4):. (PMID: 29378716)
Antimicrob Agents Chemother. 2003 Oct;47(10):3260-9. (PMID: 14506039)
Clin Microbiol Rev. 2012 Jan;25(1):2-41. (PMID: 22232370)
J Antimicrob Chemother. 2000 Mar;45(3):363-5. (PMID: 10702558)
Antimicrob Agents Chemother. 2004 Mar;48(3):946-53. (PMID: 14982788)
Int J Antimicrob Agents. 2004 Aug;24(2):173-7. (PMID: 15288317)
J Glob Antimicrob Resist. 2018 Mar;12:104-106. (PMID: 28964955)
Antimicrob Agents Chemother. 2019 Oct 22;63(11):. (PMID: 31427300)
Open Forum Infect Dis. 2022 Mar 21;9(5):ofac095. (PMID: 35415194)
J Antimicrob Chemother. 2021 Dec 24;77(1):164-168. (PMID: 34542637)
فهرسة مساهمة: Keywords: Stenotrophomonas maltophilia; acidic environment; delafloxacin; epithelial lining fluid; fluoroquinolone; levofloxacin; pH; pharmacodynamics; pneumonia; susceptibility testing; time-kill assay
المشرفين على المادة: 0 (Anti-Bacterial Agents)
0 (Fluoroquinolones)
6315412YVF (delafloxacin)
6GNT3Y5LMF (Levofloxacin)
تواريخ الأحداث: Date Created: 20220721 Date Completed: 20220908 Latest Revision: 20231020
رمز التحديث: 20231020
مُعرف محوري في PubMed: PMC9431699
DOI: 10.1128/spectrum.02705-21
PMID: 35862943
قاعدة البيانات: MEDLINE
الوصف
تدمد:2165-0497
DOI:10.1128/spectrum.02705-21