دورية أكاديمية
أعرض في EDS

Novel Insights into bla GES Mobilome Reveal Extensive Genetic Variation in Hospital Effluents.

التفاصيل البيبلوغرافية
العنوان: Novel Insights into bla GES Mobilome Reveal Extensive Genetic Variation in Hospital Effluents.
المؤلفون: Conte D; Faculdades Pequeno Príncipe (FPP), Curitiba, Paraná, Brazil.; Instituto de Pesquisa Pelé Pequeno Príncipe (IPPPP), Curitiba, Paraná, Brazil., Mesa D; Faculdades Pequeno Príncipe (FPP), Curitiba, Paraná, Brazil.; Instituto de Pesquisa Pelé Pequeno Príncipe (IPPPP), Curitiba, Paraná, Brazil., Jové T; University of Limoges, INSERM, CHU Limoges, RESINFIT, Limoges, France., Zamparette CP; Laboratório de Microbiologia Molecular Aplicada, Universidade Federal de Santa Catarina (UFSC), Florianópolis, Santa Catarina, Brazil., Sincero TCM; Departamento de Análises Clínicas, Universidade Federal de Santa Catarina (ACL-UFSC), Florianópolis, Santa Catarina, Brazil.; Laboratório de Microbiologia Molecular Aplicada, Universidade Federal de Santa Catarina (UFSC), Florianópolis, Santa Catarina, Brazil., Palmeiro JK; Instituto de Pesquisa Pelé Pequeno Príncipe (IPPPP), Curitiba, Paraná, Brazil.; Departamento de Análises Clínicas, Universidade Federal de Santa Catarina (ACL-UFSC), Florianópolis, Santa Catarina, Brazil.; Laboratório de Microbiologia Molecular Aplicada, Universidade Federal de Santa Catarina (UFSC), Florianópolis, Santa Catarina, Brazil., Dalla-Costa LM; Faculdades Pequeno Príncipe (FPP), Curitiba, Paraná, Brazil.; Instituto de Pesquisa Pelé Pequeno Príncipe (IPPPP), Curitiba, Paraná, Brazil.
المصدر: Microbiology spectrum [Microbiol Spectr] 2022 Aug 31; Vol. 10 (4), pp. e0246921. Date of Electronic Publication: 2022 Jul 26.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: ASM Press Country of Publication: United States NLM ID: 101634614 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2165-0497 (Electronic) Linking ISSN: 21650497 NLM ISO Abbreviation: Microbiol Spectr Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, DC : ASM Press, 2013-
مواضيع طبية MeSH: Aeromonas*/genetics , beta-Lactamases*/genetics, Animals ; Anti-Bacterial Agents/pharmacology ; Drug Resistance, Multiple, Bacterial/genetics ; Genetic Variation ; Hospitals ; Humans ; Klebsiella pneumoniae/genetics ; Microbial Sensitivity Tests ; Multilocus Sequence Typing ; Plasmids/genetics
مستخلص: Mobile genetic elements contribute to the emergence and spread of multidrug-resistant bacteria by enabling the horizontal transfer of acquired antibiotic resistance among different bacterial species and genera. This study characterizes the genetic backbone of bla GES in Aeromonas spp. and Klebsiella spp. isolated from untreated hospital effluents. Plasmids ranging in size from 9 to 244 kb, sequenced using Illumina and Nanopore platforms, revealed representatives of plasmid incompatibility groups IncP6, IncQ1, IncL/M1, IncFII, and IncFII-FIA. Different GES enzymes (GES-1, GES-7, and GES-16) were located in novel class 1 integrons in Aeromonas spp. and GES-5 in previously reported class 1 integrons in Klebsiella spp. Furthermore, in Klebsiella quasipneumoniae, bla GES-5 was found in tandem as a coding sequence that disrupted the 3' conserved segment (CS). In Klebsiella grimontii, bla GES-5 was observed in two different plasmids, and one of them carried multiple IncF replicons. Three Aeromonas caviae isolates presented bla GES-1 , one Aeromonas veronii isolate presented bla GES-7 , and another A. veronii isolate presented bla GES-16 . Multilocus sequence typing (MLST) analysis revealed novel sequence types for Aeromonas and Klebsiella species. The current findings highlight the large genetic diversity of these species, emphasizing their great adaptability to the environment. The results also indicate a public health risk because these antimicrobial-resistant genes have the potential to reach wastewater treatment plants and larger water bodies. Considering that they are major interfaces between humans and the environment, they could spread throughout the community to clinical settings. IMPORTANCE In the "One Health" approach, which encompasses human, animal, and environmental health, emerging issues of antimicrobial resistance are associated with hospital effluents that contain clinically relevant antibiotic-resistant bacteria along with a wide range of antibiotic concentrations, and lack regulatory status for mandatory prior and effective treatment. bla GES genes have been reported in aquatic environments despite the low detection of these genes among clinical isolates within the studied hospitals. Carbapenemase enzymes, which are relatively unusual globally, such as GES type inserted into new integrons on plasmids, are worrisome. Notably, K. grimontii, a newly identified species, carried two plasmids with bla GES-5 , and K. quasipneumoniae carried two copies of bla GES-5 at the same plasmid. These kinds of plasmids are primarily responsible for multidrug resistance among bacteria in both clinical and natural environments, and they harbor resistant genes against antibiotics of key importance in clinical therapy, possibly leading to a public health problem of large proportion.
References: Diagn Microbiol Infect Dis. 2018 Oct;92(2):147-151. (PMID: 29861147)
Methods Mol Med. 1998;15:33-50. (PMID: 21390741)
Antimicrob Agents Chemother. 2010 Nov;54(11):4872-8. (PMID: 20805394)
Antimicrob Agents Chemother. 2009 Sep;53(9):3908-13. (PMID: 19596871)
Antimicrob Agents Chemother. 2000 Mar;44(3):622-32. (PMID: 10681329)
BMC Genomics. 2008 Feb 08;9:75. (PMID: 18261238)
Trends Microbiol. 2021 Jan;29(1):65-83. (PMID: 32448764)
Nucleic Acids Res. 1997 Sep 1;25(17):3389-402. (PMID: 9254694)
Bioinformatics. 2000 Oct;16(10):944-5. (PMID: 11120685)
Front Microbiol. 2016 Feb 19;7:173. (PMID: 26925045)
Antimicrob Agents Chemother. 2020 Aug 20;64(9):. (PMID: 32631822)
J Antimicrob Chemother. 2010 Dec;65(12):2518-29. (PMID: 20935300)
J Enzyme Inhib Med Chem. 2017 Dec;32(1):917-919. (PMID: 28719998)
Antimicrob Agents Chemother. 2009 Jun;53(6):2492-8. (PMID: 19332679)
Plasmid. 2012 Jan;67(1):15-34. (PMID: 22037393)
Antimicrob Agents Chemother. 2003 Apr;47(4):1481-2. (PMID: 12654700)
Plasmid. 1999 Sep;42(2):73-91. (PMID: 10489325)
Appl Environ Microbiol. 2012 Aug;78(15):5413-6. (PMID: 22582073)
Ecotoxicol Environ Saf. 2017 Feb;136:62-69. (PMID: 27816836)
Plasmid. 2015 Mar;78:79-87. (PMID: 25102058)
Antimicrob Agents Chemother. 2010 Mar;54(3):1331-3. (PMID: 20065056)
Clin Microbiol Rev. 2007 Jul;20(3):440-58, table of contents. (PMID: 17630334)
Antimicrob Agents Chemother. 2005 Nov;49(11):4809-10. (PMID: 16251340)
J Antimicrob Chemother. 2002 Mar;49(3):561-5. (PMID: 11864961)
Bioinformatics. 2014 Aug 1;30(15):2114-20. (PMID: 24695404)
Diagn Microbiol Infect Dis. 2007 Aug;58(4):465-8. (PMID: 17467221)
J Glob Antimicrob Resist. 2021 Jun;25:287-309. (PMID: 33895415)
J Antimicrob Chemother. 2010 Aug;65(8):1664-71. (PMID: 20542902)
Arq Neuropsiquiatr. 2014 May;72(5):398-9. (PMID: 24863522)
Environ Pollut. 2020 May;260:113913. (PMID: 31972417)
Antimicrob Agents Chemother. 2006 Jan;50(1):178-84. (PMID: 16377684)
Antimicrob Agents Chemother. 2011 Mar;55(3):1256-61. (PMID: 21149627)
Front Cell Infect Microbiol. 2020 Oct 26;10:563482. (PMID: 33194801)
PLoS Genet. 2010 Jan;6(1):e1000793. (PMID: 20066027)
Diagn Microbiol Infect Dis. 2019 Aug;94(4):321-325. (PMID: 31029489)
Infect Drug Resist. 2018 Sep 20;11:1523-1536. (PMID: 30288063)
BMC Genomics. 2011 Aug 08;12:402. (PMID: 21824423)
Clin Microbiol Rev. 2018 Aug 1;31(4):. (PMID: 30068738)
J Comput Biol. 2012 May;19(5):455-77. (PMID: 22506599)
J Glob Antimicrob Resist. 2021 Sep;26:230-232. (PMID: 34273594)
mSphere. 2020 Sep 2;5(5):. (PMID: 32878926)
J Antimicrob Chemother. 2010 Aug;65(8):1594-8. (PMID: 20525990)
Front Microbiol. 2019 Sep 12;10:2132. (PMID: 31572337)
Infect Control Hosp Epidemiol. 2006 Nov;27(11):1153-8. (PMID: 17080370)
Clin Infect Dis. 2007 Jan 15;44(2):280-6. (PMID: 17173232)
Clin Infect Dis. 2020 Dec 17;71(10):2553-2560. (PMID: 31746994)
Nucleic Acids Res. 1995 Jun 11;23(11):1990-6. (PMID: 7596828)
Antimicrob Agents Chemother. 2013 May;57(5):2397-400. (PMID: 23459483)
Diagn Microbiol Infect Dis. 2021 Feb;99(2):115239. (PMID: 33130509)
J Antimicrob Chemother. 2007 Sep;60(3):470-82. (PMID: 17595289)
Rev Infect Dis. 1988 Jul-Aug;10(4):867-78. (PMID: 3263690)
PLoS One. 2017 Jun 6;12(6):e0179169. (PMID: 28586403)
Front Microbiol. 2019 Jul 23;10:1669. (PMID: 31396186)
Rev Soc Bras Med Trop. 2015 Mar-Apr;48(2):162-9. (PMID: 25992930)
Antimicrob Agents Chemother. 2016 Jan 04;60(3):1928-31. (PMID: 26729503)
Bioinformatics. 2009 Apr 15;25(8):1096-8. (PMID: 19228805)
Int J Antimicrob Agents. 2015 Feb;45(2):174-7. (PMID: 25499185)
Bioinformatics. 2014 Jul 15;30(14):2068-9. (PMID: 24642063)
J Appl Microbiol. 2021 Jul;131(1):169-181. (PMID: 33306232)
Front Microbiol. 2018 Sep 11;9:2170. (PMID: 30271396)
فهرسة مساهمة: Keywords: ESBL; MLST; carbapenemase; integrons; multireplicons
المشرفين على المادة: 0 (Anti-Bacterial Agents)
EC 3.5.2.6 (beta-Lactamases)
تواريخ الأحداث: Date Created: 20220726 Date Completed: 20220908 Latest Revision: 20240903
رمز التحديث: 20240903
مُعرف محوري في PubMed: PMC9430818
DOI: 10.1128/spectrum.02469-21
PMID: 35880869
قاعدة البيانات: MEDLINE
الوصف
تدمد:2165-0497
DOI:10.1128/spectrum.02469-21