دورية أكاديمية
أعرض في EDS

An AuNPs-Based Fluorescent Sensor with Truncated Aptamer for Detection of Sulfaquinoxaline in Water.

التفاصيل البيبلوغرافية
العنوان: An AuNPs-Based Fluorescent Sensor with Truncated Aptamer for Detection of Sulfaquinoxaline in Water.
المؤلفون: Chen X; College of Life Science, Chongqing Normal University, Chongqing 401331, China., Yang L; College of Life Science, Chongqing Normal University, Chongqing 401331, China., Tang J; College of Life Science, Chongqing Normal University, Chongqing 401331, China., Wen X; College of Life Science, Chongqing Normal University, Chongqing 401331, China., Zheng X; College of Life Science, Chongqing Normal University, Chongqing 401331, China., Chen L; College of Life Science, Chongqing Normal University, Chongqing 401331, China., Li J; College of Life Science, Chongqing Normal University, Chongqing 401331, China., Xie Y; Bioassay 3D Reconstruction Laboratory, Chongqing College of Electronic Engineering, Chongqing 401331, China., Le T; College of Life Science, Chongqing Normal University, Chongqing 401331, China.
المصدر: Biosensors [Biosensors (Basel)] 2022 Jul 11; Vol. 12 (7). Date of Electronic Publication: 2022 Jul 11.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Pub Country of Publication: Switzerland NLM ID: 101609191 Publication Model: Electronic Cited Medium: Internet ISSN: 2079-6374 (Electronic) Linking ISSN: 20796374 NLM ISO Abbreviation: Biosensors (Basel) Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI Pub.
مواضيع طبية MeSH: Aptamers, Nucleotide*/chemistry , Biosensing Techniques*/methods , Metal Nanoparticles*/chemistry, Gold/chemistry ; Limit of Detection ; Molecular Docking Simulation ; Sulfaquinoxaline ; Water
مستخلص: Herein, we developed a novel truncation technique for aptamer sequences to fabricate highly sensitive aptasensors based on molecular docking and molecular dynamics simulations. The binding mechanism and energy composition of the aptamer/sulfaquinoxaline (SQX) complexes were investigated. We successfully obtained a new SQX-specific aptamer (SBA28-1: CCCTAGGGG) with high affinity (K d = 27.36 nM) and high specificity determined using graphene oxide. This aptamer has a unique stem-loop structure that can bind to SQX. Then, we fabricated a fluorescence aptasensor based on SBA28-1, gold nanoparticles (AuNPs), and rhodamine B (RhoB) that presented a good linear range of 1.25-160 ng/mL and a limit of detection of 1.04 ng/mL. When used to analyze water samples, the aptasensor presented acceptable recovery rates of 93.1-100.1% and coefficients of variation (CVs) of 2.2-10.2%. In conclusion, the fluorescence aptasensor can accurately and sensitively detect SQX in water samples and has good application prospects.
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معلومات مُعتمدة: No. 31671939 National Natural Science Foundation of China; CYS21273 Postgraduate Scientific Research and Innovation Project of Chongqing Municipal Education Commission; No. CXQT20031 Chongqing of University Innovation Research Group Project; cstc2021jcyj-msmX0314 Chongqing Natural Science Foundation project
فهرسة مساهمة: Keywords: aptamer truncation; fluorescence aptasensor; gold nanoparticles; molecular docking; molecular dynamics simulations
المشرفين على المادة: 0 (Aptamers, Nucleotide)
059QF0KO0R (Water)
7440-57-5 (Gold)
WNW8115TM9 (Sulfaquinoxaline)
تواريخ الأحداث: Date Created: 20220727 Date Completed: 20220728 Latest Revision: 20220731
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC9312917
DOI: 10.3390/bios12070513
PMID: 35884316
قاعدة البيانات: MEDLINE
الوصف
تدمد:2079-6374
DOI:10.3390/bios12070513