دورية أكاديمية
أعرض في EDS

Impact of Exogenous Application of Potato Virus Y-Specific dsRNA on RNA Interference, Pattern-Triggered Immunity and Poly(ADP-ribose) Metabolism.

التفاصيل البيبلوغرافية
العنوان: Impact of Exogenous Application of Potato Virus Y-Specific dsRNA on RNA Interference, Pattern-Triggered Immunity and Poly(ADP-ribose) Metabolism.
المؤلفون: Samarskaya VO; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, Russia., Spechenkova N; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, Russia., Markin N; Doka-Gene Technologies Ltd., 141880 Rogachevo, Russia., Suprunova TP; Doka-Gene Technologies Ltd., 141880 Rogachevo, Russia., Zavriev SK; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, Russia., Love AJ; The James Hutton Institute, Invergowrie, Dundee DD2 5DA, UK., Kalinina NO; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, Russia.; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991 Moscow, Russia., Taliansky M; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, Russia.; The James Hutton Institute, Invergowrie, Dundee DD2 5DA, UK.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2022 Jul 18; Vol. 23 (14). Date of Electronic Publication: 2022 Jul 18.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: Potyvirus*/physiology , Solanum tuberosum*/metabolism, Plant Diseases/genetics ; Poly Adenosine Diphosphate Ribose ; RNA Interference ; RNA, Double-Stranded/genetics ; RNA, Small Interfering/genetics ; RNA, Small Interfering/metabolism
مستخلص: In this work we developed and exploited a spray-induced gene silencing (SIGS)-based approach to deliver double-stranded RNA (dsRNA), which was found to protect potato against potato virus Y (PVY) infection. Given that dsRNA can act as a defence-inducing signal that can trigger sequence-specific RNA interference (RNAi) and non-specific pattern-triggered immunity (PTI), we suspected that these two pathways may be invoked via exogeneous application of dsRNA, which may account for the alterations in PVY susceptibility in dsRNA-treated potato plants. Therefore, we tested the impact of exogenously applied PVY-derived dsRNA on both these layers of defence (RNAi and PTI) and explored its effect on accumulation of a homologous virus (PVY) and an unrelated virus (potato virus X, PVX). Here, we show that application of PVY dsRNA in potato plants induced accumulation of both small interfering RNAs (siRNAs), a hallmark of RNAi, and some PTI-related gene transcripts such as WRKY29 (WRKY transcription factor 29; molecular marker of PTI), RbohD (respiratory burst oxidase homolog D), EDS5 (enhanced disease susceptibility 5), SERK3 (somatic embryogenesis receptor kinase 3) encoding brassinosteroid-insensitive 1-associated receptor kinase 1 (BAK1), and PR-1b (pathogenesis-related gene 1b). With respect to virus infections, PVY dsRNA suppressed only PVY replication but did not exhibit any effect on PVX infection in spite of the induction of PTI-like effects in the presence of PVX. Given that RNAi-mediated antiviral immunity acts as the major virus resistance mechanism in plants, it can be suggested that dsRNA-based PTI alone may not be strong enough to suppress virus infection. In addition to RNAi- and PTI-inducing activities, we also showed that PVY-specific dsRNA is able to upregulate production of a key enzyme involved in poly(ADP-ribose) metabolism, namely poly(ADP-ribose) glycohydrolase (PARG), which is regarded as a positive regulator of biotic stress responses. These findings offer insights for future development of innovative approaches which could integrate dsRNA-induced RNAi, PTI and modulation of poly(ADP-ribose) metabolism in a co-ordinated manner, to ensure a high level of crop protection.
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معلومات مُعتمدة: 22-14-00049 Russian Science Foundation
فهرسة مساهمة: Keywords: RNA interference; pattern-triggered immunity; poly(ADP-ribose) metabolism; potato virus Y
المشرفين على المادة: 0 (RNA, Double-Stranded)
0 (RNA, Small Interfering)
26656-46-2 (Poly Adenosine Diphosphate Ribose)
تواريخ الأحداث: Date Created: 20220727 Date Completed: 20220728 Latest Revision: 20220731
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC9317112
DOI: 10.3390/ijms23147915
PMID: 35887257
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms23147915