دورية أكاديمية
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TbpB Y167A -Based Vaccine Can Protect Pigs against Glässer's Disease Triggered by Glaesserella parasuis SV7 Expressing TbpB Cluster I.

التفاصيل البيبلوغرافية
العنوان: TbpB Y167A -Based Vaccine Can Protect Pigs against Glässer's Disease Triggered by Glaesserella parasuis SV7 Expressing TbpB Cluster I.
المؤلفون: Ramos Prigol S; Laboratory of Microbiology and Advanced Immunology, Faculty of Agronomy and Veterinary Medicine, University of Passo Fundo, Passo Fundo 99052-900, Rio Grande do Sul, Brazil.; AFK Imunotech Ltd.a, Passo Fundo 99050-144, Rio Grande do Sul, Brazil., Klein R; Laboratory of Microbiology and Advanced Immunology, Faculty of Agronomy and Veterinary Medicine, University of Passo Fundo, Passo Fundo 99052-900, Rio Grande do Sul, Brazil., Chaudhuri S; Department of Microbiology and Infectious Diseases, Faculty of Medicine, University of Calgary, Calgary, AB T2N 4N1, Canada., Paraboni Frandoloso G; AFK Imunotech Ltd.a, Passo Fundo 99050-144, Rio Grande do Sul, Brazil., Guizzo JA; AFK Imunotech Ltd.a, Passo Fundo 99050-144, Rio Grande do Sul, Brazil., Gutiérrez Martín CB; Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de León, Campus de Vegazana s/n, 24007 Leon, Spain., Schryvers AB; Department of Microbiology and Infectious Diseases, Faculty of Medicine, University of Calgary, Calgary, AB T2N 4N1, Canada., Kreutz LC; Laboratory of Microbiology and Advanced Immunology, Faculty of Agronomy and Veterinary Medicine, University of Passo Fundo, Passo Fundo 99052-900, Rio Grande do Sul, Brazil., Frandoloso R; Laboratory of Microbiology and Advanced Immunology, Faculty of Agronomy and Veterinary Medicine, University of Passo Fundo, Passo Fundo 99052-900, Rio Grande do Sul, Brazil.
المصدر: Pathogens (Basel, Switzerland) [Pathogens] 2022 Jul 04; Vol. 11 (7). Date of Electronic Publication: 2022 Jul 04.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101596317 Publication Model: Electronic Cited Medium: Print ISSN: 2076-0817 (Print) Linking ISSN: 20760817 NLM ISO Abbreviation: Pathogens Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI AG, 2012-
مستخلص: Glaesserella parasuis is the etiological agent of Glässer's disease (GD), one of the most important diseases afflicting pigs in the nursery phase. We analyzed the genetic and immunological properties of the TbpB protein naturally expressed by 27 different clinical isolates of G. parasuis that were typed as serovar 7 and isolated from pigs suffering from GD. All the strains were classified as virulent by LS-PCR. The phylogenetic analyses demonstrated high similarity within the amino acid sequence of TbpB from 24 clinical strains all belonging to cluster III of TbpB, as does the protective antigen TbpB Y167A . Three G. parasuis isolates expressed cluster I TbpBs, indicating antigenic diversity within the SV7 group of G. parasuis . The antigenic analysis demonstrated the presence of common epitopes on all variants of the TbpB protein, which could be recognized by an in vitro analysis using pig IgG induced by a TbpB Y167A -based vaccine. The proof of concept of the complete cross-protection between clusters I and III was performed in SPF pigs immunized with the TbpB Y167A -based vaccine (cluster III) and challenged with G. parasuis SV7, strains LM 360.18 (cluster I). Additionally, pigs immunized with a whole-cell inactivated vaccine based on G. parasuis SV5 (Nagasaki strain) did not survive the challenge performed with SV7 (strain 360.18), demonstrating the absence of cross-protection between these two serovars. Based on these results, we propose that a properly formulated TbpB Y167A -based vaccine may elicit a protective antibody response against all strains of G. parasuis SV7, despite TbpB antigenic diversity, and this might be extrapolated to other serovars. This result highlights the promising use of the TbpB Y167A antigen in a future commercial vaccine for GD prevention.
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فهرسة مساهمة: Keywords: Glaesserella parasuis; Glässer’s disease; TbpBY167A; cross-protection; serovar 7; vaccine
تواريخ الأحداث: Date Created: 20220727 Latest Revision: 20220731
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC9323293
DOI: 10.3390/pathogens11070766
PMID: 35890011
قاعدة البيانات: MEDLINE
الوصف
تدمد:2076-0817
DOI:10.3390/pathogens11070766