دورية أكاديمية
أعرض في EDS

Small Extracellular Vesicles from Inflamed Adipose Derived Stromal Cells Enhance the NF- κ B-Dependent Inflammatory/Catabolic Environment of Osteoarthritis.

التفاصيل البيبلوغرافية
العنوان: Small Extracellular Vesicles from Inflamed Adipose Derived Stromal Cells Enhance the NF- κ B-Dependent Inflammatory/Catabolic Environment of Osteoarthritis.
المؤلفون: Cavallo C; Laboratorio RAMSES, IRCCS Istituto Ortopedico Rizzoli, Bologna 40136, Italy., Merli G; Applied and Translational Research Center (ATRc), IRCCS Istituto Ortopedico Rizzoli, Bologna 40136, Italy., Zini N; CNR Institute of Molecular Genetics 'Luigi Luca Cavalli-Sforza', Unit of Bologna, Bologna 40136, Italy.; IRCCS Istituto Ortopedico Rizzoli, Bologna 40136, Italy., D'Adamo S; Department of Medical and Surgical Sciences, University of Bologna, Bologna 40138, Italy.; Laboratorio di Immunoreumatologia e Rigenerazione Tissutale, IRCCS Istituto Ortopedico Rizzoli, Bologna 40136, Italy., Cattini L; Laboratorio di Immunoreumatologia e Rigenerazione Tissutale, IRCCS Istituto Ortopedico Rizzoli, Bologna 40136, Italy., Guescini M; Department of Biomolecular Science, University of Urbino Carlo Bo, Urbino 61029, Italy., Grigolo B; Laboratorio RAMSES, IRCCS Istituto Ortopedico Rizzoli, Bologna 40136, Italy., Di Martino A; Clinica Ortopedica e Traumatologica 2, IRCCS Istituto Ortopedico Rizzoli, Bologna 40136, Italy., Santi S; CNR Institute of Molecular Genetics 'Luigi Luca Cavalli-Sforza', Unit of Bologna, Bologna 40136, Italy.; IRCCS Istituto Ortopedico Rizzoli, Bologna 40136, Italy., Borzì RM; Laboratorio di Immunoreumatologia e Rigenerazione Tissutale, IRCCS Istituto Ortopedico Rizzoli, Bologna 40136, Italy., Filardo G; Applied and Translational Research Center (ATRc), IRCCS Istituto Ortopedico Rizzoli, Bologna 40136, Italy.
المصدر: Stem cells international [Stem Cells Int] 2022 Jul 18; Vol. 2022, pp. 9376338. Date of Electronic Publication: 2022 Jul 18 (Print Publication: 2022).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Hindawi Country of Publication: United States NLM ID: 101535822 Publication Model: eCollection Cited Medium: Print ISSN: 1687-966X (Print) NLM ISO Abbreviation: Stem Cells Int Subsets: PubMed not MEDLINE
أسماء مطبوعة: Publication: 2011- : New York : Hindawi
Original Publication: London, England ; New York, NY : SAGE-Hindawi Access to Research
مستخلص: The last decade has seen exponentially growing efforts to exploit the effects of adipose derived stromal cells (ADSC) in the treatment of a wide range of chronic degenerative diseases, including osteoarthritis (OA), the most prevalent joint disorder. In the perspective of developing a cell-free advanced therapy medicinal product, a focus has been recently addressed to the ADSC secretome that lends itself to an allogeneic use and can be further dissected for the selective purification of small extracellular vesicles (sEVs). sEVs can act as "biological drug carriers" to transfer information that mirror the pathophysiology of the providing cells. This is important in the clinical perspective where many OA patients are also affected by the metabolic syndrome (MetS). ADSC from MetS OA patients are dysfunctional and "inflammatory" primed within the adipose tissue. To mimic this condition, we exposed ADSC to IL-1 β , and then we investigated the effects of the isolated sEVs on chondrocytes and synoviocytes, either cultured separately or in co-culture, to tease out the effects of these "IL-1 β primed sEVs" on gene and protein expression of major inflammatory and catabolic OA markers. In comparison with sEVs isolated from unstimulated ADSC, the IL-1 β primed sEVs were able to propagate NF- κ B activation in bystander joint cells. The effects were more prominent on synoviocytes, possibly because of a higher expression of binding molecules such as CD44. These findings call upon a careful characterization of the "inflammatory fingerprint" of ADSC to avoid the transfer of an unwanted message as well as the development of in vitro "preconditioning" strategies able to rescue the antiinflammatory/anticatabolic potential of ADSC-derived sEVs.
Competing Interests: The authors declare that there is no conflict of interest regarding the publication of this paper.
(Copyright © 2022 Carola Cavallo et al.)
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تواريخ الأحداث: Date Created: 20220728 Latest Revision: 20220729
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC9314187
DOI: 10.1155/2022/9376338
PMID: 35898656
قاعدة البيانات: MEDLINE
الوصف
تدمد:1687-966X
DOI:10.1155/2022/9376338