دورية أكاديمية
أعرض في EDS

Single-cell transcriptomics of staged oocytes and somatic cells reveal novel regulators of follicle activation.

التفاصيل البيبلوغرافية
العنوان: Single-cell transcriptomics of staged oocytes and somatic cells reveal novel regulators of follicle activation.
المؤلفون: Chen YY; Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA., Russo DD; Ragon Institute of MGH, MIT, and Harvard, Cambridge, Massachusetts, USA.; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Institute of Medical Science and Engineering, Department of Chemistry, and Koch Institute for Integrative Cancer Research, MIT, Cambridge, Massachusetts, USA., Drake RS; Ragon Institute of MGH, MIT, and Harvard, Cambridge, Massachusetts, USA.; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Institute of Medical Science and Engineering, Department of Chemistry, and Koch Institute for Integrative Cancer Research, MIT, Cambridge, Massachusetts, USA., Duncan FE; Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA., Shalek AK; Ragon Institute of MGH, MIT, and Harvard, Cambridge, Massachusetts, USA.; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.; Institute of Medical Science and Engineering, Department of Chemistry, and Koch Institute for Integrative Cancer Research, MIT, Cambridge, Massachusetts, USA., Goods BA; The Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire, USA., Woodruff TK; Department of Obstetrics and Gynecology, Michigan State University, East Lansing, Michigan, USA.
المصدر: Reproduction (Cambridge, England) [Reproduction] 2022 Jul 07; Vol. 164 (2), pp. 55-70. Date of Electronic Publication: 2022 Jul 07 (Print Publication: 2022).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Published for the Society for Reproduction and Fertility by BioScientifica Country of Publication: England NLM ID: 100966036 Publication Model: Electronic-Print Cited Medium: Internet ISSN: 1741-7899 (Electronic) Linking ISSN: 14701626 NLM ISO Abbreviation: Reproduction Subsets: MEDLINE
أسماء مطبوعة: Publication: <2004->: Bristol, UK : Published for the Society for Reproduction and Fertility by BioScientifica
Original Publication: Cambridge, UK : Journals of Reproduction and Fertility, Ltd. c2001-
مواضيع طبية MeSH: Ovarian Follicle* , Transcriptome*, Animals ; Female ; Granulosa Cells ; Mammals ; Mice ; Oocytes ; Ovary/metabolism
مستخلص: In Brief: Proper development of ovarian follicles, comprised of an oocyte and surrounding somatic cells, is essential to support female fertility and endocrine health. Here, we describe a method to isolate single oocytes and somatic cells from the earliest stage follicles, called primordial follicles, and we characterize signals that drive their activation.
Abstract: Primordial follicles are the first class of follicles formed in the mammalian ovary and are comprised of an oocyte surrounded by a layer of squamous pre-granulosa cells. This developmental class remains in a non-growing state until individual follicles activate to initiate folliculogenesis. What regulates the timing of follicle activation and the upstream signals that govern these processes are major unanswered questions in ovarian biology. This is partly due to the paucity of data on staged follicle cells since isolating and manipulating individual oocytes and somatic cells from early follicle stages are challenging. To date, most studies on isolated primordial follicles have been conducted on cells collected from animal-age- or oocyte size-specific samples, which encompass multiple follicular stages. Here, we report a method for collecting primordial follicles and their associated oocytes and somatic cells from neonatal murine ovaries using liberase, DNase I, and Accutase. This methodology allows for the identification and collection of follicles immediately post-activation enabling unprecedented interrogation of the primordial-to-primary follicle transition. Molecular profiling by single-cell RNA sequencing revealed that processes including organelle disassembly and cadherin binding were enriched in oocytes and somatic cells as they transitioned from primordial to the primary follicle stage. Furthermore, targets including WNT4, TGFB1, FOXO3, and a network of transcription factors were identified in the transitioning oocytes and somatic cells as potential upstream regulators that collectively may drive follicle activation. Taken together, we have developed a more precise characterization and selection method for studying staged-follicle cells, revealing several novel regulators of early folliculogenesis.
References: Proc Natl Acad Sci U S A. 2007 Jan 2;104(1):187-92. (PMID: 17189423)
J Vis Exp. 2011 Mar 15;(49):. (PMID: 21445043)
Reprod Biol Endocrinol. 2016 Dec 5;14(1):82. (PMID: 27919266)
Reprod Biomed Online. 2015 Feb;30(2):181-90. (PMID: 25530035)
Tissue Eng. 2006 Oct;12(10):2739-46. (PMID: 17518643)
Dev Biol. 2001 Dec 15;240(2):488-98. (PMID: 11784078)
Anim Reprod Sci. 2007 Sep;101(1-2):163-71. (PMID: 17250982)
Mol Cell. 2018 Dec 20;72(6):1021-1034.e4. (PMID: 30472193)
Proc Natl Acad Sci U S A. 2013 Oct 22;110(43):17474-9. (PMID: 24082083)
Biol Reprod. 2012 May 17;86(5):153, 1-14. (PMID: 22321830)
BMC Bioinformatics. 2013 Apr 15;14:128. (PMID: 23586463)
Mech Dev. 2000 Mar 1;91(1-2):61-8. (PMID: 10704831)
Dev Biol. 2006 Oct 1;298(1):132-48. (PMID: 16930587)
J Assist Reprod Genet. 2018 Jul;35(7):1135-1148. (PMID: 29691711)
J Reprod Fertil. 1989 Nov;87(2):561-71. (PMID: 2689642)
Mol Hum Reprod. 2021 Feb 5;27(2):. (PMID: 33538812)
Genes Dev. 1997 Sep 1;11(17):2153-62. (PMID: 9303532)
J Reprod Dev. 2020 Apr 10;66(2):105-113. (PMID: 31902808)
Genome Biol. 2015 Sep 25;16:209. (PMID: 26408185)
Mol Cell. 2017 Feb 16;65(4):631-643.e4. (PMID: 28212749)
Genome Biol. 2014;15(12):550. (PMID: 25516281)
Proc Natl Acad Sci U S A. 2006 May 23;103(21):8090-5. (PMID: 16690745)
Science. 2004 Aug 20;305(5687):1157-9. (PMID: 15326356)
Mol Hum Reprod. 2009 Dec;15(12):795-803. (PMID: 19710243)
Nat Commun. 2020 Jan 16;11(1):330. (PMID: 31949138)
J Assist Reprod Genet. 2014 Aug;31(8):1013-28. (PMID: 24845158)
Genome Biol. 2020 Jun 2;21(1):130. (PMID: 32487174)
Biol Reprod. 2022 Mar 19;106(3):503-514. (PMID: 34673933)
Curr Protoc Mol Biol. 2014 Jul 01;107:4.22.1-17. (PMID: 24984854)
Cell. 2021 Jun 24;184(13):3573-3587.e29. (PMID: 34062119)
Hum Reprod Update. 2015 Nov-Dec;21(6):779-86. (PMID: 26231759)
Biol Reprod. 2015 Oct;93(4):88. (PMID: 26246221)
Dev Biol. 2001 Jun 15;234(2):339-51. (PMID: 11397004)
Hum Reprod. 1999 May;14(5):1299-301. (PMID: 10325281)
Tissue Eng Part A. 2020 Jul;26(13-14):712-719. (PMID: 32598233)
Int J Mol Sci. 2021 Jun 18;22(12):. (PMID: 34207376)
Sci Rep. 2020 Mar 3;10(1):3906. (PMID: 32127571)
Curr Protoc. 2021 Mar;1(3):e90. (PMID: 33780170)
PLoS Biol. 2020 Dec 22;18(12):e3001025. (PMID: 33351795)
J Ovarian Res. 2017 Oct 23;10(1):71. (PMID: 29061149)
Proc Natl Acad Sci U S A. 2019 May 14;116(20):9775-9784. (PMID: 31028141)
J Cell Sci. 2008 Dec 1;121(Pt 23):3890-900. (PMID: 19001500)
Hum Reprod. 2012 Jun;27(6):1801-10. (PMID: 22456922)
Hum Reprod. 2006 Feb;21(2):413-20. (PMID: 16199426)
Sci Rep. 2019 Apr 24;9(1):6513. (PMID: 31015579)
Mol Reprod Dev. 2004 Aug;68(4):422-8. (PMID: 15236325)
Proc Natl Acad Sci U S A. 2019 Jun 18;116(25):12321-12326. (PMID: 31147464)
Biomaterials. 2007 Oct;28(30):4439-48. (PMID: 17643486)
Curr Biol. 2014 Nov 3;24(21):2501-8. (PMID: 25438940)
Development. 2021 May 1;148(9):. (PMID: 33914868)
Endocrinology. 2015 Apr;156(4):1464-76. (PMID: 25594701)
Nucleic Acids Res. 2016 Jul 8;44(W1):W90-7. (PMID: 27141961)
J Cell Sci. 2018 Sep 7;131(17):. (PMID: 30111581)
Fertil Steril. 1997 Mar;67(3):481-6. (PMID: 9091334)
J Biol Chem. 2014 Mar 21;289(12):8299-311. (PMID: 24515103)
Nat Commun. 2019 Apr 3;10(1):1523. (PMID: 30944313)
Mech Dev. 2002 Feb;111(1-2):137-41. (PMID: 11804785)
Dev Biol. 2005 Oct 15;286(2):493-506. (PMID: 16168984)
Syst Biol Reprod Med. 2018 Feb;64(1):3-11. (PMID: 29224376)
Front Oncol. 2019 Feb 11;9:60. (PMID: 30805310)
Sci Rep. 2015 Dec 03;5:17709. (PMID: 26633657)
Dev Biol. 2008 Sep 1;321(1):197-204. (PMID: 18601916)
Fertil Steril. 2011 Aug;96(2):379-383.e3. (PMID: 21719006)
تواريخ الأحداث: Date Created: 20220728 Date Completed: 20220729 Latest Revision: 20220926
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC9354060
DOI: 10.1530/REP-22-0053
PMID: 35899878
قاعدة البيانات: MEDLINE
الوصف
تدمد:1741-7899
DOI:10.1530/REP-22-0053