دورية أكاديمية
أعرض في EDS

Rapid Adaptation of Established High-Throughput Molecular Testing Infrastructure for Monkeypox Virus Detection.

التفاصيل البيبلوغرافية
العنوان: Rapid Adaptation of Established High-Throughput Molecular Testing Infrastructure for Monkeypox Virus Detection.
المؤلفون: Nörz D, Tang HT, Emmerich P, Giersch K, Fischer N, Schmiedel S, Addo MM, Aepfelbacher M, Pfefferle S, Lütgehetmann M
المصدر: Emerging infectious diseases [Emerg Infect Dis] 2022 Sep; Vol. 28 (9), pp. 1765-1769. Date of Electronic Publication: 2022 Jul 29.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: National Center for Infectious Diseases, Centers for Disease Control and Prevention (CDC) Country of Publication: United States NLM ID: 9508155 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1080-6059 (Electronic) Linking ISSN: 10806040 NLM ISO Abbreviation: Emerg Infect Dis Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Atlanta, GA : National Center for Infectious Diseases, Centers for Disease Control and Prevention (CDC), [1995-
مواضيع طبية MeSH: COVID-19* , Mpox (monkeypox)*/diagnosis , Mpox (monkeypox)*/epidemiology, Humans ; Molecular Diagnostic Techniques ; Monkeypox virus/genetics ; SARS-CoV-2/genetics ; Sensitivity and Specificity
مستخلص: Beginning in May 2022, a rising number of monkeypox cases were reported in non-monkeypox-endemic countries in the Northern Hemisphere. We adapted 2 published quantitative PCRs for use as a dual-target monkeypox virus test on widely used automated high-throughput PCR systems. We determined analytic performance by serial dilutions of monkeypox virus reference material, which we quantified by digital PCR. We found the lower limit of detection for the combined assays was 4.795 (95% CI 3.6-8.6) copies/mL. We compared clinical performance against a commercial manual orthopoxvirus research use only PCR kit by using clinical remnant swab samples. Our assay showed 100% positive (n = 11) and 100% negative (n = 56) agreement. Timely and scalable PCR tests are crucial for limiting further spread of monkeypox. The assay we provide streamlines high-throughput molecular testing for monkeypox virus on existing broadly established platforms used for SARS-CoV-2 diagnostic testing.
References: Euro Surveill. 2020 Jan;25(3):. (PMID: 31992387)
Ann Intern Med. 2022 Aug;175(8):1175-1176. (PMID: 35605243)
PLoS Negl Trop Dis. 2022 Feb 11;16(2):e0010141. (PMID: 35148313)
Lancet Infect Dis. 2022 Aug;22(8):1153-1162. (PMID: 35623380)
Lancet. 1988 Apr 2;1(8588):769-70. (PMID: 2895299)
Euro Surveill. 2022 Jun;27(22):. (PMID: 35656836)
N Engl J Med. 2004 Jan 22;350(4):342-50. (PMID: 14736926)
Euro Surveill. 2020 Mar;25(9):. (PMID: 32156329)
J Clin Microbiol. 2020 May 26;58(6):. (PMID: 32277022)
Euro Surveill. 2022 Jun;27(22):. (PMID: 35656830)
J Clin Virol. 2006 Jul;36(3):194-203. (PMID: 16731033)
J Virol Methods. 2011 Aug;175(2):163-9. (PMID: 21635922)
Euro Surveill. 2022 Jun;27(22):. (PMID: 35656834)
فهرسة مساهمة: Keywords: Germany; SARS-CoV-2; cobas; high-throughput PCR testing; molecular diagnostics; monkeypox virus; viruses; zoonoses
تواريخ الأحداث: Date Created: 20220729 Date Completed: 20220823 Latest Revision: 20231213
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC9423910
DOI: 10.3201/eid2809.220917
PMID: 35905463
قاعدة البيانات: MEDLINE
الوصف
تدمد:1080-6059
DOI:10.3201/eid2809.220917