دورية أكاديمية
أعرض في EDS

A molecular dyad delivered by biodegradable polymeric nanoparticles for combined PDT and NO-PDT in cancer cells.

التفاصيل البيبلوغرافية
العنوان: A molecular dyad delivered by biodegradable polymeric nanoparticles for combined PDT and NO-PDT in cancer cells.
المؤلفون: Parisi C; PhotoChemLab, Department of Drug and Health Sciences, University of Catania, I-95125 Catania, Italy., Longobardi G; Drug Delivery Laboratory, Department of Pharmacy, University of Napoli Federico II, I-80131 Napoli, Italy., Graziano ACE; PhotoChemLab, Department of Drug and Health Sciences, University of Catania, I-95125 Catania, Italy., Fraix A; PhotoChemLab, Department of Drug and Health Sciences, University of Catania, I-95125 Catania, Italy., Conte C; Drug Delivery Laboratory, Department of Pharmacy, University of Napoli Federico II, I-80131 Napoli, Italy., Quaglia F; Drug Delivery Laboratory, Department of Pharmacy, University of Napoli Federico II, I-80131 Napoli, Italy. Electronic address: quaglia@unina.it., Sortino S; PhotoChemLab, Department of Drug and Health Sciences, University of Catania, I-95125 Catania, Italy. Electronic address: ssortino@unict.it.
المصدر: Bioorganic chemistry [Bioorg Chem] 2022 Nov; Vol. 128, pp. 106050. Date of Electronic Publication: 2022 Jul 22.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: United States NLM ID: 1303703 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2120 (Electronic) Linking ISSN: 00452068 NLM ISO Abbreviation: Bioorg Chem Subsets: MEDLINE
أسماء مطبوعة: Publication: Amsterdam : Elsevier
Original Publication: New York, London, Academic Press.
مواضيع طبية MeSH: Nanoparticles*/chemistry , Neoplasms* , Photochemotherapy*, Cell Line, Tumor ; Nitric Oxide ; Photosensitizing Agents/chemistry ; Photosensitizing Agents/pharmacology ; Photosensitizing Agents/therapeutic use ; Polymers/chemistry ; Water
مستخلص: The design, synthesis, photochemical properties, and biological evaluation of a novel molecular dyad with double photodynamic action and its formulation within biodegradable polymeric nanoparticles (NPs) are reported. A BODIPY-based singlet oxygen ( 1 O 2 ) photosensitizer (PS) and a nitric oxide (NO) photodonor (NOPD) based on an amino-nitro-benzofurazan moiety have been covalently joined in a new molecular dyad, through a flexible alkyl spacer. Excitation of the dyad with visible light in the range 400-570 nm leads to the concomitant generation of the cytotoxic 1 O 2 and NO with effective quantum yields, being Φ Δ  = 0.49 ± 0.05 and Φ NO  = 0.18 ± 0.01, respectively. Besides, the non-fluorescent NOPD unit becomes highly fluorescent after the NO release, acting as an optical reporter for the NO photogenerated. The dyad is not soluble in water medium but can be effectively entrapped in water-dispersible, biodegradable polymeric NPs made of mPEG-PCL, ca. 66 nm in diameter. The polymeric nano-environment affects in an opposite way the photochemical performances of the dyad, reducing Φ Δ to 0.16 ± 0.02 and increasing Φ NO to 0.92 ± 0.03, respectively. The NPs effectively deliver the photoactive cargo into the cytoplasm of HepG2 hepatocellular carcinoma cells. A remarkable level of cell mortality is observed for the loaded NPs at very low concentrations of the dyad (1-5 µM) and very low light doses (≤0.8 J cm -2 ) more likely as the result of the combined photodynamic action of 1 O 2 and NO.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: Light; Multimodal therapy; Nitric oxide; Photodynamic therapy; Polymer nanoparticles
المشرفين على المادة: 0 (Photosensitizing Agents)
0 (Polymers)
059QF0KO0R (Water)
31C4KY9ESH (Nitric Oxide)
تواريخ الأحداث: Date Created: 20220730 Date Completed: 20220913 Latest Revision: 20220916
رمز التحديث: 20240628
DOI: 10.1016/j.bioorg.2022.106050
PMID: 35907377
قاعدة البيانات: MEDLINE
الوصف
تدمد:1090-2120
DOI:10.1016/j.bioorg.2022.106050