دورية أكاديمية
Proteomic Profile of Procoagulant Extracellular Vesicles Reflects Complement System Activation and Platelet Hyperreactivity of Patients with Severe COVID-19.
| العنوان: | Proteomic Profile of Procoagulant Extracellular Vesicles Reflects Complement System Activation and Platelet Hyperreactivity of Patients with Severe COVID-19. |
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| المؤلفون: | Moraes ECDS; Laboratory of Toxinology, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil.; Laboratory of Immunopharmacology, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil., Martins-Gonçalves R; Laboratory of Immunopharmacology, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil., da Silva LR; Laboratory of Toxinology, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil.; Center for Technological Development in Health, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil., Mandacaru SC; Center for Technological Development in Health, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil., Melo RM; Laboratory Protein Chemistry and Biochemistry and Laboratory of Gene Biology, Department of Cell Biology, University of Brasília, Brasília, Brazil., Azevedo-Quintanilha I; Laboratory of Immunopharmacology, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil., Perales J; Laboratory of Toxinology, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil., Bozza FA; National Institute of Infectious Disease Evandro Chagas, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.; D'Or Institute for Research and Education, Rio de Janeiro, Brazil., Souza TML; Laboratory of Immunopharmacology, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil.; Center for Technological Development in Health, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil., Castro-Faria-Neto HC; Laboratory of Immunopharmacology, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil., Hottz ED; Laboratory of Immunothrombosis, Department of Biochemistry, Federal University of Juiz de Fora, Juiz de Fora, Brazil., Bozza PT; Laboratory of Immunopharmacology, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil., Trugilho MRO; Laboratory of Toxinology, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil.; Center for Technological Development in Health, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil. |
| المصدر: | Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2022 Jul 22; Vol. 12, pp. 926352. Date of Electronic Publication: 2022 Jul 22 (Print Publication: 2022). |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Media SA Country of Publication: Switzerland NLM ID: 101585359 Publication Model: eCollection Cited Medium: Internet ISSN: 2235-2988 (Electronic) Linking ISSN: 22352988 NLM ISO Abbreviation: Front Cell Infect Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Media SA |
| مواضيع طبية MeSH: | COVID-19* , Extracellular Vesicles*/metabolism, Humans ; Proteome/metabolism ; Proteomics/methods ; SARS-CoV-2 ; Tandem Mass Spectrometry |
| مستخلص: | Background: Extracellular vesicles (EVs) are a valuable source of biomarkers and display the pathophysiological status of various diseases. In COVID-19, EVs have been explored in several studies for their ability to reflect molecular changes caused by SARS-CoV-2. Here we provide insights into the roles of EVs in pathological processes associated with the progression and severity of COVID-19. Methods: In this study, we used a label-free shotgun proteomic approach to identify and quantify alterations in EV protein abundance in severe COVID-19 patients. We isolated plasma extracellular vesicles from healthy donors and patients with severe COVID-19 by size exclusion chromatography (SEC). Then, flow cytometry was performed to assess the origin of EVs and to investigate the presence of circulating procoagulant EVs in COVID-19 patients. A total protein extraction was performed, and samples were analyzed by nLC-MS/MS in a Q-Exactive HF-X. Finally, computational analysis was applied to signify biological processes related to disease pathogenesis. Results: We report significant changes in the proteome of EVs from patients with severe COVID-19. Flow cytometry experiments indicated an increase in total circulating EVs and with tissue factor (TF) dependent procoagulant activity. Differentially expressed proteins in the disease groups were associated with complement and coagulation cascades, platelet degranulation, and acute inflammatory response. Conclusions: The proteomic data reinforce the changes in the proteome of extracellular vesicles from patients infected with SARS-CoV-2 and suggest a role for EVs in severe COVID-19. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Moraes, Martins-Gonçalves, Silva, Mandacaru, Melo, Azevedo-Quintanilha, Perales, Bozza, Souza, Castro-Faria-Neto, Hottz, Bozza and Trugilho.) |
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| فهرسة مساهمة: | Keywords: Extracellular vesicles; LC-MS/MS; SARS-CoV-2; complement factors; platelets; proteome; tissue factor |
| المشرفين على المادة: | 0 (Proteome) |
| تواريخ الأحداث: | Date Created: 20220808 Date Completed: 20220809 Latest Revision: 20220817 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC9354812 |
| DOI: | 10.3389/fcimb.2022.926352 |
| PMID: | 35937696 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2235-2988 |
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| DOI: | 10.3389/fcimb.2022.926352 |