دورية أكاديمية
Technical note: Evaluation of sampling methods for methane concentration from in vitro fermentation.
| العنوان: | Technical note: Evaluation of sampling methods for methane concentration from in vitro fermentation. |
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| المؤلفون: | D'Souza GM; Department of Animal Science, Texas A&M University, College Station, TX 77843-2471, USA., Norris AB; Department of Natural Resources Management, Texas Tech University, Lubbock, TX 79409, USA., Tedeschi LO; Department of Animal Science, Texas A&M University, College Station, TX 77843-2471, USA. |
| المصدر: | Journal of animal science [J Anim Sci] 2022 Sep 01; Vol. 100 (9). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society of Animal Science Country of Publication: United States NLM ID: 8003002 Publication Model: Print Cited Medium: Internet ISSN: 1525-3163 (Electronic) Linking ISSN: 00218812 NLM ISO Abbreviation: J Anim Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Champaign, IL : American Society of Animal Science |
| مواضيع طبية MeSH: | Methane*/metabolism , Steam*, Animal Feed/analysis ; Animals ; Cattle ; Diet ; Fermentation ; Rumen/metabolism |
| مستخلص: | The objectives of this multipart study were 1) to assess the efficacy of sampling methods of methane concentration ([CH4]) of headspace gas produced during in vitro gas production (IVGP) fermentation, 2) to verify whether headspace [CH4] sampled from an exetainer has the same [CH4] as the headspace of IVGP bottles, 3) to measure relative humidity (RH) within an IVGP bottle, and 4) to compare [CH4] on a dry-gas (DG) basis when accounting for water vapor pressure (Pw). The original IVGP protocol recommends placing bottles on ice (0 °C) for 30 min to stop fermentation (ICE). A laboratory protocol recommends placing the bottles in the refrigerator (4 to 6 °C) to slow fermentation for 48 h and subsequently allowing the bottles to return to ambient temperature before sampling (FRIDGE). This study evaluated the previous methods against a direct sampling of the headspace gas after incubation (DIRECT). Rumen inoculum from four rumen-cannulated beef steers was combined and homogenized before incubating the fermentable substrate of ground alfalfa hay. After 48 h of IVGP incubation, each bottle was randomly assigned to a treatment protocol. The pressure (P), volume (V), and temperature (T) of headspace gas in each bottle were recorded. Headspace gas was then thoroughly mixed, and 12 mL gas was removed into an evacuated exetainer for [CH4] sampling via gas chromatography (EXET; Objective 1). Eight bottles from ICE and FRIDGE were randomly selected to follow EXET, whereas the remaining bottles had [CH4] directly measured from their headspace (BOTT; Objective 2). Five diets of differing feed composition and nutrient densities were used with a blank to test the RH of the IVGP slurry (Objective 3). Using RH, [CH4] was transformed to a DG basis to account for Pw (Objective 4). Statistical analysis was completed using a random coefficients model. There were no differences between EXET and BOTT (P = 0.28). The RH of the IVGP slurry was 100% (P = 1.00), confirming that IVGP gas is saturated with water vapor. The P, V, and T differed among treatments (P < 0.01). The [CH4] of DIRECT, ICE, and FRIDGE were different (P < 0.01). Dry-gas P, V, and [CH4] differed among treatments (P < 0.01). As the methods differ in their assessment of [CH4], there is no clear recommendation. Instead, to present a more accurate [CH4], P, V, and T should be measured when sampling headspace gas and equations presented should be used to remove volume inflation due to water vapor and present [CH4] on a DG basis. (© The Author(s) 2022. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.) |
| References: | J Dairy Sci. 1993 Apr;76(4):1063-73. (PMID: 8486838) Br J Nutr. 1976 Jul;36(1):1-14. (PMID: 949464) J Dairy Sci. 2009 Jan;92(1):401-13. (PMID: 19109298) Trends Biotechnol. 2016 Jan;34(1):26-35. (PMID: 26603286) Water Sci Technol. 2001;44(8):149-56. (PMID: 11730130) J Anim Physiol Anim Nutr (Berl). 2005 Apr-Jun;89(3-6):158-65. (PMID: 15787988) Animals (Basel). 2012 Apr 13;2(2):160-83. (PMID: 26486915) Animals (Basel). 2019 Oct 21;9(10):. (PMID: 31640130) J Dairy Sci. 2012 Jun;95(6):3166-80. (PMID: 22612952) |
| معلومات مُعتمدة: | Texas A&M AgriLife Research; #09123 USDA-NIFA Hatch |
| فهرسة مساهمة: | Keywords: cattle; headspace gas; in vitro gas production; methane; ruminant Local Abstract: [plain-language-summary] Greenhouse gas emissions (GHG) from ruminant production equate to 81% of total global livestock supply chain emissions, with 51% originating from beef cattle production. Traditional in vivo estimation methods of methane (CH4), a highly scrutinized greenhouse gas, are timely and costly. In vitro gas production (IVGP) methods can accurately describe CH4 emission patterns from the rumen but tend to overestimate quantities. Additionally, in vivo estimation methods present CH4 on a dry-gas basis, whereas in vitro do not. In vitro methods utilize a gas chromatography machine to estimate CH4. Laboratory constraints can impose deviations to a strict IVGP protocol. This multi-objective study evaluates three treatment methods of IVGP bottles to understand whether discrepancies exist in CH4 estimation when deviating from the published protocol. To estimate CH4 from IVGP more accurately and provide a more comparable number to in vivo methods, this study also evaluates environmental conditions within an IVGP bottle to formulate a system of equations to calculate CH4 on a dry-gas basis. This study found that the treatment method of the IVGP bottle had an impact on CH4 estimation, and the developed equations should be utilized to produce more comparable estimates. |
| المشرفين على المادة: | 0 (Steam) OP0UW79H66 (Methane) |
| تواريخ الأحداث: | Date Created: 20220811 Date Completed: 20220928 Latest Revision: 20230813 |
| رمز التحديث: | 20230813 |
| مُعرف محوري في PubMed: | PMC9512091 |
| DOI: | 10.1093/jas/skac259 |
| PMID: | 35953208 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1525-3163 |
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| DOI: | 10.1093/jas/skac259 |