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Megahertz pulse trains enable multi-hit serial femtosecond crystallography experiments at X-ray free electron lasers.

التفاصيل البيبلوغرافية
العنوان: Megahertz pulse trains enable multi-hit serial femtosecond crystallography experiments at X-ray free electron lasers.
المؤلفون: Holmes S; Department of Mathematical and Physical Sciences, School of Engineering, Computing and Mathematical Sciences, La Trobe University, Melbourne, VIC, 3086, Australia.; La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086, Australia., Kirkwood HJ; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Bean R; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Giewekemeyer K; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Martin AV; School of Science, RMIT University, Melbourne, VIC, 3000, Australia., Hadian-Jazi M; Department of Mathematical and Physical Sciences, School of Engineering, Computing and Mathematical Sciences, La Trobe University, Melbourne, VIC, 3086, Australia.; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.; Australian Nuclear Science and Technology Organisation (ANSTO), Sydney, NSW, 2234, Australia., Wiedorn MO; Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany., Oberthür D; Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany., Marman H; Department of Mathematical and Physical Sciences, School of Engineering, Computing and Mathematical Sciences, La Trobe University, Melbourne, VIC, 3086, Australia.; La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086, Australia., Adriano L; Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany., Al-Qudami N; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Bajt S; Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.; The Hamburg Centre for Ultrafast Imaging, Luruper Chaussee 149, Hamburg, 22761, Germany., Barák I; Institute of Molecular Biology, SAS, Dubravska cesta 21, 845 51, Bratislava, Slovakia., Bari S; Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany., Bielecki J; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Brockhauser S; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Coleman MA; Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA, 94550, USA., Cruz-Mazo F; Dept. de Ingeniería Aeroespacial y Mecánica de Fluidos, ETSI, Universidad de Sevilla, 41092, Sevilla, Spain.; Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ, 08544, USA., Danilevski C; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Dörner K; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Gañán-Calvo AM; Dept. de Ingeniería Aeroespacial y Mecánica de Fluidos, ETSI, Universidad de Sevilla, 41092, Sevilla, Spain., Graceffa R; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Fanghor H; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.; Max-Planck Institute for the Structure and Dynamics of Matter, Luruper Chaussee 175, 22761, Hamburg, Germany.; University of Southampton, Southampton, SO17 1BJ, UK., Heymann M; Institute of Biomaterials and Biomolecular Systems, University of Stuttgart, Am Pfaffenwaldring 57, 70569, Stuttgart, Germany., Frank M; Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA, 94550, USA., Kaukher A; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Kim Y; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Kobe B; School of Chemistry and Molecular Biosciences, Institute for Molecular Bioscience and Australian Infectious Diseases Research Centre, University of Queensland, Brisbane, QLD, 4072, Australia., Knoška J; Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.; Department of Physics, Universität Hamburg, Luruper Chaussee 149, 22761, Hamburg, Germany., Laurus T; Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany., Letrun R; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Maia L; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Messerschmidt M; School of Molecular Science, Arizona State University, Tempe, AZ, 85281, USA., Metz M; Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany., Michelat T; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Mills G; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Molodtsov S; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.; Institute of Experimental Physics, TU Bergakademie Freiberg, Leipziger, Str. 23, 09599, Freiberg, Germany.; ITMO University, Kronverksky pr. 49, St. Petersburg, 197101, Russia., Monteiro DCF; The Hamburg Centre for Ultrafast Imaging, Luruper Chaussee 149, Hamburg, 22761, Germany.; Hauptman-Woodward Medical Research Institute, 700 Ellicott St., Buffalo, NY, 14203, USA., Morgan AJ; Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.; Department of Physics, University of Melbourne, Parkville, VIC, 3010, Australia., Münnich A; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Peña Murillo GE; Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany., Previtali G; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Round A; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Sato T; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.; Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany., Schubert R; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Schulz J; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Shelby M; Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA, 94550, USA., Seuring C; Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.; The Hamburg Centre for Ultrafast Imaging, Luruper Chaussee 149, Hamburg, 22761, Germany., Sellberg JA; Biomedical and X-ray Physics, Department of Applied Physics, AlbaNova University Center, KTH Royal Institute of Technology, SE-106 91, Stockholm, Sweden., Sikorski M; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Silenzi A; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Stern S; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Sztuk-Dambietz J; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Szuba J; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Trebbin M; Institute of Experimental Physics, TU Bergakademie Freiberg, Leipziger, Str. 23, 09599, Freiberg, Germany.; Department of Chemistry, State University of New York at Buffalo, 760 Natural Sciences Complex, Buffalo, NY, 14260, USA., Vagovic P; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Ve T; Institute for Glycomics, Griffith University, Southport, QLD, 4222, Australia., Weinhausen B; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Wrona K; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Xavier PL; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.; Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.; Max-Planck Institute for the Structure and Dynamics of Matter, Luruper Chaussee 175, 22761, Hamburg, Germany., Xu C; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Yefanov O; Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany., Nugent KA; Department of Quantum Science and Technology, Research School of Physics, Australian National University, Canberra, ACT, 2601, Australia., Chapman HN; Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.; The Hamburg Centre for Ultrafast Imaging, Luruper Chaussee 149, Hamburg, 22761, Germany.; Department of Physics, Universität Hamburg, Luruper Chaussee 149, 22761, Hamburg, Germany., Mancuso AP; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.; La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086, Australia., Barty A; Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany., Abbey B; Department of Mathematical and Physical Sciences, School of Engineering, Computing and Mathematical Sciences, La Trobe University, Melbourne, VIC, 3086, Australia. b.abbey@latrobe.edu.au.; La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086, Australia. b.abbey@latrobe.edu.au., Darmanin C; Department of Mathematical and Physical Sciences, School of Engineering, Computing and Mathematical Sciences, La Trobe University, Melbourne, VIC, 3086, Australia. c.darmanin@latrobe.edu.au.; La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086, Australia. c.darmanin@latrobe.edu.au.
المصدر: Nature communications [Nat Commun] 2022 Aug 11; Vol. 13 (1), pp. 4708. Date of Electronic Publication: 2022 Aug 11.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Pub. Group
مواضيع طبية MeSH: Electrons* , Lasers*, Crystallography, X-Ray ; Radiography ; X-Rays
مستخلص: The European X-ray Free Electron Laser (XFEL) and Linac Coherent Light Source (LCLS) II are extremely intense sources of X-rays capable of generating Serial Femtosecond Crystallography (SFX) data at megahertz (MHz) repetition rates. Previous work has shown that it is possible to use consecutive X-ray pulses to collect diffraction patterns from individual crystals. Here, we exploit the MHz pulse structure of the European XFEL to obtain two complete datasets from the same lysozyme crystal, first hit and the second hit, before it exits the beam. The two datasets, separated by <1 µs, yield up to 2.1 Å resolution structures. Comparisons between the two structures reveal no indications of radiation damage or significant changes within the active site, consistent with the calculated dose estimates. This demonstrates MHz SFX can be used as a tool for tracking sub-microsecond structural changes in individual single crystals, a technique we refer to as multi-hit SFX.
(© 2022. The Author(s).)
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معلومات مُعتمدة: R01 GM117342 United States GM NIGMS NIH HHS
تواريخ الأحداث: Date Created: 20220811 Date Completed: 20220815 Latest Revision: 20230329
رمز التحديث: 20230329
مُعرف محوري في PubMed: PMC9372077
DOI: 10.1038/s41467-022-32434-6
PMID: 35953469
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-1723
DOI:10.1038/s41467-022-32434-6