دورية أكاديمية
Epidemiological Cutoff Values for Standard Broth Microdilution and Disk Diffusion Susceptibility Testing of Aeromonas hydrophila Isolated from Fish.
| العنوان: | Epidemiological Cutoff Values for Standard Broth Microdilution and Disk Diffusion Susceptibility Testing of Aeromonas hydrophila Isolated from Fish. |
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| المؤلفون: | Gieseker CM; Food and Drug Administration, Center for Veterinary Medicine, Office of Research, Laurel, Maryland, USA., Gaunt PS; Thad Cochran National Warmwater Aquaculture Center, College of Veterinary Medicine, Mississippi State University, Stoneville, Mississippi, USA., Hawke JP; Department of Pathobiological Science, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana, USA., Crosby TC; Food and Drug Administration, Center for Veterinary Medicine, Office of Research, Laurel, Maryland, USA., Hasbrouck NR; Food and Drug Administration, Center for Veterinary Medicine, Office of Research, Laurel, Maryland, USA., Gao DX; Thad Cochran National Warmwater Aquaculture Center, College of Veterinary Medicine, Mississippi State University, Stoneville, Mississippi, USA., Stine CB; Food and Drug Administration, Center for Veterinary Medicine, Office of Research, Laurel, Maryland, USA., Evans ER; Food and Drug Administration, Center for Veterinary Medicine, Office of Research, Laurel, Maryland, USA., Grim CJ; Food and Drug Administration, Center for Food Safety and Applied Nutrition, Office of Applied Research and Safety Assessment, Laurel, Maryland, USA. |
| المصدر: | Microbial drug resistance (Larchmont, N.Y.) [Microb Drug Resist] 2022 Aug; Vol. 28 (8), pp. 893-903. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Mary Ann Liebert Country of Publication: United States NLM ID: 9508567 Publication Model: Print Cited Medium: Internet ISSN: 1931-8448 (Electronic) Linking ISSN: 10766294 NLM ISO Abbreviation: Microb Drug Resist Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Larchmont, N.Y. : Mary Ann Liebert, c1995- |
| مواضيع طبية MeSH: | Aeromonas*/genetics , Anti-Infective Agents*, Aeromonas hydrophila ; Animals ; Anti-Bacterial Agents/pharmacology ; Fishes ; Microbial Sensitivity Tests |
| مستخلص: | Aeromonas hydrophila and other closely related Aeromonas species cause motile aeromonad septicemia, a common fish disease. The disease affects many aquaculture sectors potentially requiring antimicrobial treatments. Therefore, researchers and laboratory diagnosticians need criteria called epidemiological cutoff values (ECVs) to determine whether a bacterial isolate has developed decreased susceptibility to an antimicrobial. To generate ECVs for this bacterium, we assembled a diverse collection of 245 isolates previously identified as A. hydrophila from fish. Using rpoD sequencing, we confirmed that 97 of the 245 isolates were A. hydrophila . We allocated the isolates among three laboratories and tested their susceptibility against eight antimicrobials using standard Clinical and Laboratory Standards Institute (CLSI) disk diffusion and broth microdilution methods. The resulting frequency distributions were statistically analyzed to determine wild-type cutoff estimates, which, along with scatterplots, were used to estimate potential ECVs. In collaboration with the CLSI, aquaculture working group, we proposed ECVs for six of the eight antimicrobials tested. Subsequently, the CLSI Subcommittee on Veterinary Antimicrobial Susceptibility Testing reviewed our data and approved the ECVs to be added to the 2020 edition of the VET04 performance standards for antimicrobial susceptibility testing of aquatic bacteria. |
| فهرسة مساهمة: | Keywords: Aeromonas hydrophila; epidemiological cutoff values; fish; motile aeromonad septicemia |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (Anti-Infective Agents) |
| تواريخ الأحداث: | Date Created: 20220816 Date Completed: 20220818 Latest Revision: 20220818 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1089/mdr.2021.0316 |
| PMID: | 35972765 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1931-8448 |
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| DOI: | 10.1089/mdr.2021.0316 |