دورية أكاديمية
أعرض في EDS

The potential role of serum expression profile of long non coding RNAs, Cox2 and HOTAIR as novel diagnostic biomarkers in systemic lupus erythematosus.

التفاصيل البيبلوغرافية
العنوان: The potential role of serum expression profile of long non coding RNAs, Cox2 and HOTAIR as novel diagnostic biomarkers in systemic lupus erythematosus.
المؤلفون: Mahmoud RH; Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Fayoum University, Fayoum, Egypt., Fouad NA; Department of Rheumatology and Rehabilitation, Faculty of Medicine, Fayoum University, Fayoum, Egypt., Hefzy EM; Department of Medical Microbiology and Immunology, Faculty of Medicine, Fayoum University, Cairo, Egypt., Shaker OG; Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt., Ahmed TI; Department of Internal Medicine, Faculty of Medicine, Fayoum University, Fayoum, Egypt., Hussein HA; Department of Internal Medicine, Faculty of Medicine, Fayoum University, Fayoum, Egypt., Nasr MH; Department of Rheumatology and Rehabilitation, Faculty of Medicine, Fayoum University, Fayoum, Egypt., Zaki OM; Department of Clinical Pathology, Faculty of Medicine, Damietta University, Damietta, Egypt., Abdelghaffar NK; Department of Clinical Pathology, Faculty of Medicine, Fayoum University, Fayoum, Egypt., Abdelaleem OO; Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Fayoum University, Fayoum, Egypt.
المصدر: PloS one [PLoS One] 2022 Aug 16; Vol. 17 (8), pp. e0268176. Date of Electronic Publication: 2022 Aug 16 (Print Publication: 2022).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Lupus Erythematosus, Systemic*/diagnosis , Lupus Erythematosus, Systemic*/genetics , Lupus Nephritis* , RNA, Long Noncoding*/genetics, Biomarkers ; Humans ; Interleukin-6/blood ; Matrix Metalloproteinase 9/blood
مستخلص: Background: The role of the long non-coding RNAs (lncRNAs) in the pathogenesis of systemic lupus erythematosus (SLE) is mostly unknown, despite increasing evidence that lncRNAs extensively participate in physiological and pathological conditions.
Aim: To detect the level of lncRNA-Cox2, HOTAIR, IL-6, and MMP-9 in the serum of SLE patients and to correlate these levels with disease activity and patients' clinical and laboratory data to evaluate the value of these biomarkers for SLE diagnosis and assessment of disease activity.
Methods: Blood samples from 58 SLE patients, and 60 healthy controls (HCs) were used for detection of lncRNAs-Cox2 and HOTAIR expression levels by real-time polymerase chain reaction. Both IL-6 and MMP-9 serum levels were assayed by enzyme-linked immunosorbent assay. Lupus activity was assessed with the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI).
Results: The serum expression levels of lncRNA-Cox2 and HOTAIR were significantly up-regulated in SLE patients vs HCs (fold change [median (IQR) was 1.29(0.81-1.71, P<0.0001) and 2.68(0.95-3.67), P = 0.038) for lncRNA-Cox2 and HOTAIR, respectively. Serum levels of both IL-6 and MMP-9 were significantly high in SLE patients compared with HCs (P≤0.001 for each). The up-regulated lncRNA-Cox2 was positively associated with the presence of neurological manifestations in SLE patients (P = 0.007). Furthermore, HOTAIR expression level had significantly positive correlation with IL-6 (r = 0.578, P<0.0001), MMP-9 level (r = 0.762, P<0.0001), nephritis grades (r = 0.296, P = 0.024) and proteinuria (r = 0.287, P = 0.035). LncRNA-Cox2 showed sensitivity and specificity 72.4%, and 100.0% respectively. HOTAIR sensitivity was 60.3%, and specificity was 100.0%. By multiple logistic regression analysis, lncRNA-Cox2 and HOTAIR were found as SLE independent predictors.
Conclusion: LncRNA-COX2 and HOTAIR can be used as new non-invasive biomarkers for the diagnosis of SLE.
Competing Interests: The authors have declared that no competing interests exist
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المشرفين على المادة: 0 (Biomarkers)
0 (HOTAIR long untranslated RNA, human)
0 (Interleukin-6)
0 (RNA, Long Noncoding)
EC 3.4.24.35 (Matrix Metalloproteinase 9)
تواريخ الأحداث: Date Created: 20220816 Date Completed: 20220818 Latest Revision: 20240902
رمز التحديث: 20240902
مُعرف محوري في PubMed: PMC9380942
DOI: 10.1371/journal.pone.0268176
PMID: 35972968
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0268176