دورية أكاديمية
An antigen in metaphase chromatin and the midbody of mammalian cells binds to scleroderma sera.
| العنوان: | An antigen in metaphase chromatin and the midbody of mammalian cells binds to scleroderma sera. |
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| المؤلفون: | Fritzler MJ, Ayer LM, Gohill J, O'Connor C, Laxer RM, Humbel RL |
| المصدر: | The Journal of rheumatology [J Rheumatol] 1987 Apr; Vol. 14 (2), pp. 291-4. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Journal Of Rheumatology Publishing Co Country of Publication: Canada NLM ID: 7501984 Publication Model: Print Cited Medium: Print ISSN: 0315-162X (Print) Linking ISSN: 0315162X NLM ISO Abbreviation: J Rheumatol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Toronto : Journal Of Rheumatology Publishing Co Original Publication: Toronto. |
| مواضيع طبية MeSH: | Antigens/*immunology , Cells/*immunology , Chromatin/*immunology , Scleroderma, Systemic/*blood, Cell Line ; Cells/ultrastructure ; Humans ; Metaphase ; Scleroderma, Systemic/immunology |
| مستخلص: | Antibodies from 5 patients with systemic sclerosis reacted with an antigen localized to the metaphase chromatin, the cleavage furrow and the midbody of anaphase and telophase HEp-2 cells. The titer of antimidbody antibodies ranged from 1:160 to 1:1280. Four patients had systemic sclerosis and one had idiopathic Raynaud's phenomenon. In situ biochemical characterization of the antigen revealed that it was resistant to DNase I, micrococcal nuclease and RNase A, but was sensitive to trypsin treatment. The antigen remained insoluble in 400 mM acetic acid but was extracted from the cells with 400 mM hydrochloric acid. The antibody was not seen in sera from 2500 normal female blood donors, 120 patients with systemic lupus, 60 patients with rheumatoid arthritis, 15 patients with linear scleroderma or 25 patients with Raynaud's disease. |
| المشرفين على المادة: | 0 (Antigens) 0 (Chromatin) |
| تواريخ الأحداث: | Date Created: 19870401 Date Completed: 19870810 Latest Revision: 20061115 |
| رمز التحديث: | 20221213 |
| PMID: | 3598998 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0315-162X |
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