دورية أكاديمية
Validation of the modified radiographic union score for tibia fractures (mRUST) in murine femoral fractures.
| العنوان: | Validation of the modified radiographic union score for tibia fractures (mRUST) in murine femoral fractures. |
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| المؤلفون: | Alentado VJ; Department of Neurological Surgery, School of Medicine, Indiana University, Indianapolis, IN, United States., Knox AM; Department of Orthopaedic Surgery, School of Medicine, Indiana University, Indianapolis, IN, United States., Staut CA; Department of Orthopaedic Surgery, School of Medicine, Indiana University, Indianapolis, IN, United States., McGuire AC; Department of Orthopaedic Surgery, School of Medicine, Indiana University, Indianapolis, IN, United States., Chitwood JR; Department of Orthopaedic Surgery, School of Medicine, Indiana University, Indianapolis, IN, United States., Mostardo SL; Department of Orthopaedic Surgery, School of Medicine, Indiana University, Indianapolis, IN, United States., Shaikh MZ; Department of Orthopaedic Surgery, School of Medicine, Indiana University, Indianapolis, IN, United States., Blosser RJ; Department of Orthopaedic Surgery, School of Medicine, Indiana University, Indianapolis, IN, United States., Dadwal UC; Department of Orthopaedic Surgery, School of Medicine, Indiana University, Indianapolis, IN, United States., Chu TG; Department of Biomedical Sciences and Comprehensive Care, School of Dentistry, Indiana University, Indianapolis, IN, United States., Collier CD; Department of Orthopaedic Surgery, School of Medicine, Indiana University, Indianapolis, IN, United States., Li J; Department of Biology, Indiana University, Purdue University, Indianapolis, IN, United States., Liu Z; Department of Biostatistics and Health Data Science, School of Public Health, Indiana University, Indianapolis, IN, United States., Kacena MA; Department of Orthopaedic Surgery, School of Medicine, Indiana University, Indianapolis, IN, United States.; Richard L. Roudebush VA Medical Center, Department of Veterans Affairs, Indianapolis, IN, United States., Natoli RM; Department of Orthopaedic Surgery, School of Medicine, Indiana University, Indianapolis, IN, United States. |
| المصدر: | Frontiers in endocrinology [Front Endocrinol (Lausanne)] 2022 Aug 03; Vol. 13, pp. 911058. Date of Electronic Publication: 2022 Aug 03 (Print Publication: 2022). |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101555782 Publication Model: eCollection Cited Medium: Print ISSN: 1664-2392 (Print) Linking ISSN: 16642392 NLM ISO Abbreviation: Front Endocrinol (Lausanne) Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Lausanne : Frontiers Research Foundation] |
| مواضيع طبية MeSH: | Femoral Fractures*/diagnostic imaging , Femoral Fractures*/surgery , Tibia*, Animals ; Bony Callus ; Fracture Healing ; Humans ; Mice ; X-Ray Microtomography |
| مستخلص: | Bony union is a primary predictor of outcome after surgical fixation of long bone fractures. Murine models offer many advantages in assessing bony healing due to their low costs and small size. However, current fracture recovery investigations in mice frequently rely on animal sacrifice and costly analyses. The modified Radiographic Union Score for Tibia fractures (mRUST) scoring system is a validated metric for evaluating bony healing in humans utilizing plain radiographs, which are relatively inexpensive and do not require animal sacrifice. However, its use has not been well established in murine models. The aim of this study was to characterize the longitudinal course of mRUST and compare mRUST to other conventional murine fracture analyses. 158 mice underwent surgically created midshaft femur fractures. Mice were evaluated after fracture creation and at 7, 10, 14, 17, 21, 24, 28, 35, and 42 days post-injury. mRUST scoring of plain radiographs was performed by three orthopaedic surgeons in a randomized, blinded fashion. Interrater correlations were calculated. Micro-computed tomography (μCT) was analyzed for tissue mineral density (TMD), total callus volume (TV), bone volume (BV), trabecular thickness, trabecular number, and trabecular separation. Histomorphometry measures of total callus area, cartilage area, fibrous tissue area, and bone area were performed in a blinded fashion. Ultimate torque, stiffness, toughness, and twist to failure were calculated from torque-twist curves. A sigmoidal log-logistic curve fit was generated for mRUST scores over time which shows mRUST scores of 4 to 6 at 7 days post-injury that improve to plateaus of 14 to 16 by 24 days post-injury. mRUST interrater correlations at each timepoint ranged from 0.51 to 0.86, indicating substantial agreement. mRUST scores correlated well with biomechanical, histomorphometry, and μCT parameters, such as ultimate torque (r=0.46, p<0.0001), manual stiffness (r=0.51, p<0.0001), bone percentage based on histomorphometry (r=0.86, p<0.0001), cartilage percentage (r=-0.87, p<0.0001), tissue mineral density (r=0.83, p<0.0001), BV/TV based on μCT (r=0.65, p<0.0001), and trabecular thickness (r=0.78, p<0.0001), among others. These data demonstrate that mRUST is reliable, trends temporally, and correlates to standard measures of murine fracture healing. Compared to other measures, mRUST is more cost-effective and non-terminal. The mRUST log-logistic curve could be used to characterize differences in fracture healing trajectory between experimental groups, enabling high-throughput analysis. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Alentado, Knox, Staut, McGuire, Chitwood, Mostardo, Shaikh, Blosser, Dadwal, Chu, Collier, Li, Liu, Kacena and Natoli.) |
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| معلومات مُعتمدة: | R01 AG060621 United States AG NIA NIH HHS; I01 RX003552 United States RX RRD VA; T35 HL110854 United States HL NHLBI NIH HHS; U54 DK106846 United States DK NIDDK NIH HHS; IK6 RX004809 United States RX RRD VA; I01 BX003751 United States BX BLRD VA; T32 AR065971 United States AR NIAMS NIH HHS |
| فهرسة مساهمة: | Keywords: bone healing; fracture biomechanics; fracture healing; histomorphometry; micro computed tomography; radiographic union score for tibial fractures |
| تواريخ الأحداث: | Date Created: 20220822 Date Completed: 20220823 Latest Revision: 20240423 |
| رمز التحديث: | 20240423 |
| مُعرف محوري في PubMed: | PMC9381990 |
| DOI: | 10.3389/fendo.2022.911058 |
| PMID: | 35992150 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1664-2392 |
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| DOI: | 10.3389/fendo.2022.911058 |