دورية أكاديمية
Gold Nanoparticle Smartphone Platform for Diagnosing Urinary Tract Infections.
العنوان: | Gold Nanoparticle Smartphone Platform for Diagnosing Urinary Tract Infections. |
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المؤلفون: | Zagorovsky K; Institute of Biomedical Engineering, University of Toronto, 164 College Street, Toronto, Ontario M5S 3G9, Canada.; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada., Fernández-Argüelles MT; Institute of Biomedical Engineering, University of Toronto, 164 College Street, Toronto, Ontario M5S 3G9, Canada.; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada.; Department of Physical and Analytical Chemistry, University of Oviedo, Julian Claveria 8, Oviedo, Asturias 33006, Spain., Bona D; Department of Biochemistry, University of Toronto, 661 University Avenue, Toronto, Ontario M5G 1M1, Canada., Elshawadfy AM; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada.; Department of Botany and Microbiology, Zagazig University, Zagazig 44519, Egypt., Syed AM; Institute of Biomedical Engineering, University of Toronto, 164 College Street, Toronto, Ontario M5S 3G9, Canada.; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada.; Gladstone Institute of Data Science and Biotechnology, 1650 Owens Street, San Francisco, California 94158, United States., Kadhiresan P; Institute of Biomedical Engineering, University of Toronto, 164 College Street, Toronto, Ontario M5S 3G9, Canada.; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada., Mazzulli T; Department of Microbiology, Mount Sinai Hospital/University Health Network, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada., Maxwell KL; Department of Biochemistry, University of Toronto, 661 University Avenue, Toronto, Ontario M5G 1M1, Canada., Chan WCW; Institute of Biomedical Engineering, University of Toronto, 164 College Street, Toronto, Ontario M5S 3G9, Canada.; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada. |
المصدر: | ACS nanoscience Au [ACS Nanosci Au] 2022 Aug 17; Vol. 2 (4), pp. 324-332. Date of Electronic Publication: 2022 Apr 04. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 9918316881006676 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2694-2496 (Electronic) Linking ISSN: 26942496 NLM ISO Abbreviation: ACS Nanosci Au Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: [Columbus, OH] : American Chemical Society, [2021]- |
مستخلص: | Current urinary tract infection (UTI) diagnostic methods are slow or provide limited information, resulting in prescribing antibiotic therapy before bacterial pathogen identification. Here, we adapted a gold nanoparticle colorimetric approach and developed a smartphone platform for UTI detection. We show the parallel identification of five major UTI pathogens at clinically relevant concentrations of 10 5 bacteria/mL using bacteria-specific and universal probes. We validated the diagnostic technology using 115 positive and 19 negative samples from patients with Escherichia coli , Proteus mirabilis, and Klebsiella pneumoniae infections. The assay successfully identified the infecting pathogen (specificity: >98% and sensitivity: 51-73%) in 3 h. Our platform is faster than culturing and can wirelessly store and transmit results at the cost of $0.38 per assay. Competing Interests: The authors declare the following competing financial interest(s): We have a start company called Luna Nanotech that will commercialize this technology. (© 2022 The Authors. Published by American Chemical Society.) |
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تواريخ الأحداث: | Date Created: 20220823 Latest Revision: 20220824 |
رمز التحديث: | 20221213 |
مُعرف محوري في PubMed: | PMC9389610 |
DOI: | 10.1021/acsnanoscienceau.2c00001 |
PMID: | 35996437 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2694-2496 |
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DOI: | 10.1021/acsnanoscienceau.2c00001 |