دورية أكاديمية
أعرض في EDS

Gold Nanoparticle Smartphone Platform for Diagnosing Urinary Tract Infections.

التفاصيل البيبلوغرافية
العنوان: Gold Nanoparticle Smartphone Platform for Diagnosing Urinary Tract Infections.
المؤلفون: Zagorovsky K; Institute of Biomedical Engineering, University of Toronto, 164 College Street, Toronto, Ontario M5S 3G9, Canada.; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada., Fernández-Argüelles MT; Institute of Biomedical Engineering, University of Toronto, 164 College Street, Toronto, Ontario M5S 3G9, Canada.; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada.; Department of Physical and Analytical Chemistry, University of Oviedo, Julian Claveria 8, Oviedo, Asturias 33006, Spain., Bona D; Department of Biochemistry, University of Toronto, 661 University Avenue, Toronto, Ontario M5G 1M1, Canada., Elshawadfy AM; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada.; Department of Botany and Microbiology, Zagazig University, Zagazig 44519, Egypt., Syed AM; Institute of Biomedical Engineering, University of Toronto, 164 College Street, Toronto, Ontario M5S 3G9, Canada.; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada.; Gladstone Institute of Data Science and Biotechnology, 1650 Owens Street, San Francisco, California 94158, United States., Kadhiresan P; Institute of Biomedical Engineering, University of Toronto, 164 College Street, Toronto, Ontario M5S 3G9, Canada.; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada., Mazzulli T; Department of Microbiology, Mount Sinai Hospital/University Health Network, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada., Maxwell KL; Department of Biochemistry, University of Toronto, 661 University Avenue, Toronto, Ontario M5G 1M1, Canada., Chan WCW; Institute of Biomedical Engineering, University of Toronto, 164 College Street, Toronto, Ontario M5S 3G9, Canada.; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada.
المصدر: ACS nanoscience Au [ACS Nanosci Au] 2022 Aug 17; Vol. 2 (4), pp. 324-332. Date of Electronic Publication: 2022 Apr 04.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: American Chemical Society Country of Publication: United States NLM ID: 9918316881006676 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2694-2496 (Electronic) Linking ISSN: 26942496 NLM ISO Abbreviation: ACS Nanosci Au Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: [Columbus, OH] : American Chemical Society, [2021]-
مستخلص: Current urinary tract infection (UTI) diagnostic methods are slow or provide limited information, resulting in prescribing antibiotic therapy before bacterial pathogen identification. Here, we adapted a gold nanoparticle colorimetric approach and developed a smartphone platform for UTI detection. We show the parallel identification of five major UTI pathogens at clinically relevant concentrations of 10 5 bacteria/mL using bacteria-specific and universal probes. We validated the diagnostic technology using 115 positive and 19 negative samples from patients with Escherichia coli , Proteus mirabilis, and Klebsiella pneumoniae infections. The assay successfully identified the infecting pathogen (specificity: >98% and sensitivity: 51-73%) in 3 h. Our platform is faster than culturing and can wirelessly store and transmit results at the cost of $0.38 per assay.
Competing Interests: The authors declare the following competing financial interest(s): We have a start company called Luna Nanotech that will commercialize this technology.
(© 2022 The Authors. Published by American Chemical Society.)
References: Science. 1997 Aug 22;277(5329):1078-81. (PMID: 9262471)
Dis Mon. 2003 Feb;49(2):71-82. (PMID: 12601338)
Dtsch Arztebl Int. 2010 May;107(21):361-7. (PMID: 20539810)
J Clin Microbiol. 1989 Jun;27(6):1342-5. (PMID: 2473995)
J Clin Microbiol. 1999 May;37(5):1415-8. (PMID: 10203497)
Acad Emerg Med. 2013 Jul;20(7):631-45. (PMID: 23859578)
Nano Lett. 2009 May;9(5):1909-15. (PMID: 19344179)
Proc Natl Acad Sci U S A. 1997 Apr 29;94(9):4262-6. (PMID: 9113977)
Angew Chem Int Ed Engl. 2013 Mar 11;52(11):3168-71. (PMID: 23401222)
Small Struct. 2021 Aug;2(8):2100034. (PMID: 34230923)
Anal Chem. 2006 Dec 15;78(24):8313-8. (PMID: 17165821)
Clin Infect Dis. 2004 Apr 15;38(8):1150-8. (PMID: 15095222)
Nano Lett. 2021 Jun 23;21(12):5209-5216. (PMID: 34110166)
J Am Chem Soc. 2010 Jan 27;132(3):1051-9. (PMID: 20038095)
J Clin Microbiol. 1994 Jul;32(7):1757-62. (PMID: 7929770)
J Am Chem Soc. 2012 May 2;134(17):7266-9. (PMID: 22506486)
Nat Nanotechnol. 2013 May;8(5):369-75. (PMID: 23644570)
Chest. 2002 Jul;122(1):262-8. (PMID: 12114368)
ACS Nano. 2021 Jun 22;15(6):9379-9390. (PMID: 33970612)
Mol Microbiol. 2004 Jan;51(1):89-96. (PMID: 14651613)
Eur Respir J. 2006 Jan;27(1):158-64. (PMID: 16387949)
Chembiochem. 2010 May 3;11(7):866-79. (PMID: 20213779)
Nanoscale. 2013 Nov 7;5(21):10141-54. (PMID: 24057263)
Am J Clin Pathol. 2012 May;137(5):778-84. (PMID: 22523217)
تواريخ الأحداث: Date Created: 20220823 Latest Revision: 20220824
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC9389610
DOI: 10.1021/acsnanoscienceau.2c00001
PMID: 35996437
قاعدة البيانات: MEDLINE
الوصف
تدمد:2694-2496
DOI:10.1021/acsnanoscienceau.2c00001