دورية أكاديمية
Effect of low-level light therapy before radiotherapy in oral squamous cell carcinoma: An in vitro study.
| العنوان: | Effect of low-level light therapy before radiotherapy in oral squamous cell carcinoma: An in vitro study. |
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| المؤلفون: | Tabosa ATL; Department of Dentistry, Universidade Estadual de Montes Claros (Unimontes), Montes Claros, Minas Gerais, Brazil.; Hospital Universitário Clemente Faria, Laboratório de Pesquisa em Saúde, Universidade Estadual de Montes Claros, 562 Av. Cula Mangabeira Santo Expedito, Montes Claros, MG, Brazil., Souza MG; Department of Dentistry, Universidade Estadual de Montes Claros (Unimontes), Montes Claros, Minas Gerais, Brazil.; Hospital Universitário Clemente Faria, Laboratório de Pesquisa em Saúde, Universidade Estadual de Montes Claros, 562 Av. Cula Mangabeira Santo Expedito, Montes Claros, MG, Brazil., de Jesus SF; Department of Dentistry, Universidade Estadual de Montes Claros (Unimontes), Montes Claros, Minas Gerais, Brazil.; Hospital Universitário Clemente Faria, Laboratório de Pesquisa em Saúde, Universidade Estadual de Montes Claros, 562 Av. Cula Mangabeira Santo Expedito, Montes Claros, MG, Brazil., Rocha DF; Department of Dentistry, Universidade Estadual de Montes Claros (Unimontes), Montes Claros, Minas Gerais, Brazil.; Hospital Universitário Clemente Faria, Laboratório de Pesquisa em Saúde, Universidade Estadual de Montes Claros, 562 Av. Cula Mangabeira Santo Expedito, Montes Claros, MG, Brazil., Queiroz LDRP; Department of Dentistry, Universidade Estadual de Montes Claros (Unimontes), Montes Claros, Minas Gerais, Brazil.; Hospital Universitário Clemente Faria, Laboratório de Pesquisa em Saúde, Universidade Estadual de Montes Claros, 562 Av. Cula Mangabeira Santo Expedito, Montes Claros, MG, Brazil., Santos EM; Department of Dentistry, Universidade Estadual de Montes Claros (Unimontes), Montes Claros, Minas Gerais, Brazil.; Hospital Universitário Clemente Faria, Laboratório de Pesquisa em Saúde, Universidade Estadual de Montes Claros, 562 Av. Cula Mangabeira Santo Expedito, Montes Claros, MG, Brazil., Guimarães VHD; Department of Dentistry, Universidade Estadual de Montes Claros (Unimontes), Montes Claros, Minas Gerais, Brazil.; Hospital Universitário Clemente Faria, Laboratório de Pesquisa em Saúde, Universidade Estadual de Montes Claros, 562 Av. Cula Mangabeira Santo Expedito, Montes Claros, MG, Brazil., Andrade LAA; Dilson Godinho Hospital, Montes Claros, Minas Gerais, Brazil., Santos SH; Institute of Agricultural Sciences, Universidade Federal de Minas Gerais (UFMG), Montes Claros, Minas Gerais, Brazil.; Hospital Universitário Clemente Faria, Laboratório de Pesquisa em Saúde, Universidade Estadual de Montes Claros, 562 Av. Cula Mangabeira Santo Expedito, Montes Claros, MG, Brazil., de Paula AMB; Department of Dentistry, Universidade Estadual de Montes Claros (Unimontes), Montes Claros, Minas Gerais, Brazil.; Hospital Universitário Clemente Faria, Laboratório de Pesquisa em Saúde, Universidade Estadual de Montes Claros, 562 Av. Cula Mangabeira Santo Expedito, Montes Claros, MG, Brazil., de Souza PEN; Institute of Physics of the University of Brasilia, University of Brasilia, Brasília, Federal District, Brazil., Farias LC; Department of Dentistry, Universidade Estadual de Montes Claros (Unimontes), Montes Claros, Minas Gerais, Brazil.; Hospital Universitário Clemente Faria, Laboratório de Pesquisa em Saúde, Universidade Estadual de Montes Claros, 562 Av. Cula Mangabeira Santo Expedito, Montes Claros, MG, Brazil., Guimarães ALS; Department of Dentistry, Universidade Estadual de Montes Claros (Unimontes), Montes Claros, Minas Gerais, Brazil. andreluizguimaraes@gmail.com.; Dilson Godinho Hospital, Montes Claros, Minas Gerais, Brazil. andreluizguimaraes@gmail.com.; Hospital Universitário Clemente Faria, Laboratório de Pesquisa em Saúde, Universidade Estadual de Montes Claros, 562 Av. Cula Mangabeira Santo Expedito, Montes Claros, MG, Brazil. andreluizguimaraes@gmail.com. |
| المصدر: | Lasers in medical science [Lasers Med Sci] 2022 Dec; Vol. 37 (9), pp. 3527-3536. Date of Electronic Publication: 2022 Aug 24. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: England NLM ID: 8611515 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1435-604X (Electronic) Linking ISSN: 02688921 NLM ISO Abbreviation: Lasers Med Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : Springer Original Publication: London : Baillière Tindall, c1986- |
| مواضيع طبية MeSH: | Mouth Neoplasms*/radiotherapy , Mouth Neoplasms*/pathology , Carcinoma, Squamous Cell*/radiotherapy , Carcinoma, Squamous Cell*/pathology , Low-Level Light Therapy*/methods , Head and Neck Neoplasms*/radiotherapy, Humans ; Squamous Cell Carcinoma of Head and Neck/radiotherapy ; Squamous Cell Carcinoma of Head and Neck/etiology ; Reactive Oxygen Species |
| مستخلص: | Radiation therapy for head and neck squamous cell carcinoma (HNSCC) is associated with several complications. Although photobiomodulation (PBM) has radioprotective effects in normal tissue, it could also enhance the growth of neoplastic cells. Thus, the present study aimed to investigate the cellular response of oral squamous cell carcinoma with pre-exposure to low-level phototherapy before radiotherapy. SCC9, Cal-27, A431, and HaCaT cell lines were subjected to low-level light therapy and radiotherapy. The cells were treated with a single energy density (300 J/cm 2 ) of a light-emitting diode (660 nm) prior to ionizing radiation at different doses (0, 2, 4, and 6 Gy). After 24 h, wound scratch, proliferation, clonogenic cell survival, cell death, and reactive oxygen species (ROS) analyses were performed to evaluate cell response. The cell lines pre-exposed to PBM at the analyzed dosage were radiosensitive. The treatment significantly reduced cell proliferation and clonogenic cell survival. Migration and cell death assays also revealed positive results, with the treatment group showing lower rate of migration and higher cell death than did the control group. Moreover, PBM effectively increased the intracellular levels of ROS. PBM at 300 J/cm 2 is a promising radiosensitizing modality to reduce the radiation dose and avoid the intolerable side effects of radiotherapy for HNSCC, thus increasing the probability of successful treatment. However, further studies are needed to support and confirm the results. (© 2022. The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature.) |
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| فهرسة مساهمة: | Keywords: Head and neck cancer; Ionizing radiation; LED; Low-level light therapy; Photobiomodulation; Radiosensitization |
| المشرفين على المادة: | 0 (Reactive Oxygen Species) |
| تواريخ الأحداث: | Date Created: 20220824 Date Completed: 20221201 Latest Revision: 20221201 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1007/s10103-022-03632-x |
| PMID: | 36001245 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1435-604X |
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| DOI: | 10.1007/s10103-022-03632-x |