دورية أكاديمية
أعرض في EDS

Microcalorimetric Investigations of Reversible Staphylococcal Enterotoxin Unfolding.

التفاصيل البيبلوغرافية
العنوان: Microcalorimetric Investigations of Reversible Staphylococcal Enterotoxin Unfolding.
المؤلفون: Berry SC; National Center for Toxicological Research, US Food and Drug Administration, 3900 NCTR Road, Jefferson, AR 72079, USA., Triplett OA; National Center for Toxicological Research, US Food and Drug Administration, 3900 NCTR Road, Jefferson, AR 72079, USA., Yu LR; National Center for Toxicological Research, US Food and Drug Administration, 3900 NCTR Road, Jefferson, AR 72079, USA., Hart ME; National Center for Toxicological Research, US Food and Drug Administration, 3900 NCTR Road, Jefferson, AR 72079, USA., Jackson LS; Division of Food Processing Science & Technology, Center for Food Safety and Applied Nutrition, US Food and Drug Administration, 6502 S. Archer Rd., Bedford Park, IL 60501, USA., Tolleson WH; National Center for Toxicological Research, US Food and Drug Administration, 3900 NCTR Road, Jefferson, AR 72079, USA.
المصدر: Toxins [Toxins (Basel)] 2022 Aug 15; Vol. 14 (8). Date of Electronic Publication: 2022 Aug 15.
نوع المنشور: Journal Article; Research Support, U.S. Gov't, P.H.S.
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101530765 Publication Model: Electronic Cited Medium: Internet ISSN: 2072-6651 (Electronic) Linking ISSN: 20726651 NLM ISO Abbreviation: Toxins (Basel) Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel : MDPI
مواضيع طبية MeSH: Staphylococcal Food Poisoning* , Staphylococcal Infections*, Enterotoxins/genetics ; Food Microbiology ; Humans ; Leukocytes, Mononuclear ; Staphylococcus aureus/genetics
مستخلص: Staphylococcal food poisoning (SFP) is a common food-borne illness often associated with contamination during food handling. The genes for Staphylococcal enterotoxin (SE) isoforms SEA and SEB are frequently detected in human nasal Staphylococcus aureus isolates and these toxins are commonly associated with SFP. Past studies described the resistance of preformed SE proteins to heat inactivation and their reactivation upon cooling in foods. Full thermodynamic analyses for these processes have not been reported, however. The thermal stabilities of SEA, SEB, and SEH and reversibility of unfolding in simple buffers were investigated at pH 4.5 and pH 6.8 using differential scanning calorimetry (DSC). SEA and SEB unfolding was irreversible at pH 6.8 and at least partially reversible at pH 4.5 while SEH unfolding was irreversible at pH 4.5 and reversible at pH 6.8. Additional studies showed maximum refolding for SEB at pH 3.5-4.0 and diminished refolding at pH 4.5 with increasing ionic strength. SE-stimulated secretion of interferon-gamma by human peripheral blood mononuclear cells was used to assess residual SE biological activity following heat treatments using conditions matching those used for DSC studies. The biological activities of SEB and SEH exhibited greater resistance to heat inactivation than that of SEA. The residual activities of heat-treated SEB and SEH were measurable but diminished further in the presence of reconstituted nonfat dry milk adjusted to pH 4.5 or pH 6.8. To different extents, the pH and ionic strengths typical for foods influenced the thermal stabilities of SEA, SEB, and SEH and their potentials to renature spontaneously after heat treatments.
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معلومات مُعتمدة: U19 FD005322 United States FD FDA HHS
فهرسة مساهمة: Keywords: Staphylococcal enterotoxins; differential scanning calorimetry; food safety; protein unfolding
المشرفين على المادة: 0 (Enterotoxins)
تواريخ الأحداث: Date Created: 20220825 Date Completed: 20220829 Latest Revision: 20230525
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC9414061
DOI: 10.3390/toxins14080554
PMID: 36006217
قاعدة البيانات: MEDLINE
الوصف
تدمد:2072-6651
DOI:10.3390/toxins14080554