دورية أكاديمية
Microcalorimetric Investigations of Reversible Staphylococcal Enterotoxin Unfolding.
العنوان: | Microcalorimetric Investigations of Reversible Staphylococcal Enterotoxin Unfolding. |
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المؤلفون: | Berry SC; National Center for Toxicological Research, US Food and Drug Administration, 3900 NCTR Road, Jefferson, AR 72079, USA., Triplett OA; National Center for Toxicological Research, US Food and Drug Administration, 3900 NCTR Road, Jefferson, AR 72079, USA., Yu LR; National Center for Toxicological Research, US Food and Drug Administration, 3900 NCTR Road, Jefferson, AR 72079, USA., Hart ME; National Center for Toxicological Research, US Food and Drug Administration, 3900 NCTR Road, Jefferson, AR 72079, USA., Jackson LS; Division of Food Processing Science & Technology, Center for Food Safety and Applied Nutrition, US Food and Drug Administration, 6502 S. Archer Rd., Bedford Park, IL 60501, USA., Tolleson WH; National Center for Toxicological Research, US Food and Drug Administration, 3900 NCTR Road, Jefferson, AR 72079, USA. |
المصدر: | Toxins [Toxins (Basel)] 2022 Aug 15; Vol. 14 (8). Date of Electronic Publication: 2022 Aug 15. |
نوع المنشور: | Journal Article; Research Support, U.S. Gov't, P.H.S. |
اللغة: | English |
بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101530765 Publication Model: Electronic Cited Medium: Internet ISSN: 2072-6651 (Electronic) Linking ISSN: 20726651 NLM ISO Abbreviation: Toxins (Basel) Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Basel : MDPI |
مواضيع طبية MeSH: | Staphylococcal Food Poisoning* , Staphylococcal Infections*, Enterotoxins/genetics ; Food Microbiology ; Humans ; Leukocytes, Mononuclear ; Staphylococcus aureus/genetics |
مستخلص: | Staphylococcal food poisoning (SFP) is a common food-borne illness often associated with contamination during food handling. The genes for Staphylococcal enterotoxin (SE) isoforms SEA and SEB are frequently detected in human nasal Staphylococcus aureus isolates and these toxins are commonly associated with SFP. Past studies described the resistance of preformed SE proteins to heat inactivation and their reactivation upon cooling in foods. Full thermodynamic analyses for these processes have not been reported, however. The thermal stabilities of SEA, SEB, and SEH and reversibility of unfolding in simple buffers were investigated at pH 4.5 and pH 6.8 using differential scanning calorimetry (DSC). SEA and SEB unfolding was irreversible at pH 6.8 and at least partially reversible at pH 4.5 while SEH unfolding was irreversible at pH 4.5 and reversible at pH 6.8. Additional studies showed maximum refolding for SEB at pH 3.5-4.0 and diminished refolding at pH 4.5 with increasing ionic strength. SE-stimulated secretion of interferon-gamma by human peripheral blood mononuclear cells was used to assess residual SE biological activity following heat treatments using conditions matching those used for DSC studies. The biological activities of SEB and SEH exhibited greater resistance to heat inactivation than that of SEA. The residual activities of heat-treated SEB and SEH were measurable but diminished further in the presence of reconstituted nonfat dry milk adjusted to pH 4.5 or pH 6.8. To different extents, the pH and ionic strengths typical for foods influenced the thermal stabilities of SEA, SEB, and SEH and their potentials to renature spontaneously after heat treatments. |
References: | J Exp Med. 1994 Nov 1;180(5):1675-83. (PMID: 7964453) Front Microbiol. 2020 Jun 30;11:1483. (PMID: 32714310) Biochem Biophys Res Commun. 2015 Nov 6;467(1):171-7. (PMID: 26299923) Methods Cell Biol. 2008;84:115-41. (PMID: 17964930) Food Chem Toxicol. 2013 Aug;58:116-23. (PMID: 23603103) Biochemistry. 2003 Aug 12;42(31):9498-506. (PMID: 12899637) FEMS Microbiol Rev. 2012 Jul;36(4):815-36. (PMID: 22091892) Appl Microbiol. 1971 Dec;22(6):1034-40. (PMID: 5137578) Emerg Infect Dis. 2011 Jan;17(1):7-15. (PMID: 21192848) Toxins (Basel). 2019 Mar 23;11(3):. (PMID: 30909619) J Immunol. 1999 Dec 15;163(12):6686-93. (PMID: 10586065) EMBO J. 1993 Mar;12(3):861-7. (PMID: 8458342) Proteomics Clin Appl. 2017 Jan;11(1-2):. (PMID: 27634590) Biochem J. 1999 Mar 1;338 ( Pt 2):273-9. (PMID: 10024502) FEMS Microbiol Lett. 2005 Nov 15;252(2):267-72. (PMID: 16213675) Eur J Pharmacol. 2014 Jan 5;722:95-107. (PMID: 24184671) Biol Direct. 2007 Jul 02;2:18. (PMID: 17605815) J Membr Biol. 2015 Jun;248(3):383-94. (PMID: 25281329) J Food Prot. 1987 Mar;50(3):239-242. (PMID: 30965423) Int J Food Microbiol. 2007 Apr 20;115(3):369-75. (PMID: 17306397) J Biol Chem. 2000 Jan 21;275(3):1665-72. (PMID: 10636860) Crit Rev Food Sci Nutr. 2018;58(12):1943-1970. (PMID: 28362113) J Infect Dis. 1996 May;173(5):1263-7. (PMID: 8627083) Appl Microbiol. 1971 Jun;21(6):1064-6. (PMID: 4998349) EMBO J. 1995 Jul 17;14(14):3292-301. (PMID: 7628431) Int J Food Microbiol. 1990 Jan;10(1):33-42. (PMID: 2397149) Int J Food Microbiol. 1988 Dec 31;7(4):311-6. (PMID: 3275329) EMBO J. 2001 Jul 2;20(13):3306-12. (PMID: 11432818) Toxins (Basel). 2010 Jul;2(7):1751-73. (PMID: 22069659) Appl Microbiol. 1975 Nov;30(5):755-8. (PMID: 950) Clin Microbiol Rev. 2013 Jul;26(3):422-47. (PMID: 23824366) Appl Microbiol. 1973 Dec;26(6):938-42. (PMID: 4767298) J Biol Inorg Chem. 2015 Sep;20(6):921-33. (PMID: 26077813) Int J Food Microbiol. 2017 Sep 1;256:30-35. (PMID: 28582663) FEMS Microbiol Lett. 2018 Jul 1;365(13):. (PMID: 29878105) Int J Food Microbiol. 2018 Jan 16;265:23-29. (PMID: 29112896) Mol Pharm. 2015 Jan 5;12(1):179-93. (PMID: 25389571) J Mol Biol. 2000 Sep 22;302(3):527-37. (PMID: 10986116) Toxins (Basel). 2020 Sep 10;12(9):. (PMID: 32927913) PLoS One. 2017 Feb 16;12(2):e0172445. (PMID: 28207867) Epidemiol Infect. 2003 Feb;130(1):33-40. (PMID: 12613743) Biophys J. 1997 Jan;72(1):65-76. (PMID: 8994593) Can J Microbiol. 2017 Oct;63(10):834-840. (PMID: 28820948) Appl Environ Microbiol. 2005 May;71(5):2793-5. (PMID: 15870376) Front Microbiol. 2018 Mar 13;9:436. (PMID: 29662470) Toxins (Basel). 2020 Aug 20;12(9):. (PMID: 32825515) J Food Drug Anal. 2018 Apr;26(2):741-750. (PMID: 29567245) J Biol Chem. 1996 Dec 13;271(50):32212-6. (PMID: 8943278) Int J Food Microbiol. 2021 Nov 2;357:109366. (PMID: 34454396) J Dairy Sci. 1966 Feb;49(2):202-3. (PMID: 5952024) Protein Eng Des Sel. 2010 May;23(5):415-21. (PMID: 20181657) Appl Environ Microbiol. 1996 Sep;62(9):3274-6. (PMID: 8795216) Chem Phys Lipids. 1982 May;30(2-3):229-59. (PMID: 7046969) Int Arch Allergy Immunol. 2013;161(4):304-14. (PMID: 23689556) J Food Prot. 1996 Dec;59(12):1292-1299. (PMID: 31195505) J Agric Food Chem. 2010 May 12;58(9):5421-6. (PMID: 20402509) |
معلومات مُعتمدة: | U19 FD005322 United States FD FDA HHS |
فهرسة مساهمة: | Keywords: Staphylococcal enterotoxins; differential scanning calorimetry; food safety; protein unfolding |
المشرفين على المادة: | 0 (Enterotoxins) |
تواريخ الأحداث: | Date Created: 20220825 Date Completed: 20220829 Latest Revision: 20230525 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC9414061 |
DOI: | 10.3390/toxins14080554 |
PMID: | 36006217 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2072-6651 |
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DOI: | 10.3390/toxins14080554 |