دورية أكاديمية
أعرض في EDS

Effects of Extracellular Vesicles Secreted by TGFβ-Stimulated Umbilical Cord Mesenchymal Stem Cells on Skin Fibroblasts by Promoting Fibroblast Migration and ECM Protein Production.

التفاصيل البيبلوغرافية
العنوان: Effects of Extracellular Vesicles Secreted by TGFβ-Stimulated Umbilical Cord Mesenchymal Stem Cells on Skin Fibroblasts by Promoting Fibroblast Migration and ECM Protein Production.
المؤلفون: Vu DM; Vinmec Center for Applied Sciences and Regenerative Medicine, Vinmec Healthcare System, Hanoi 100000, Vietnam., Nguyen VT; Vinmec Center for Applied Sciences and Regenerative Medicine, Vinmec Healthcare System, Hanoi 100000, Vietnam., Nguyen TH; Vinmec Center for Applied Sciences and Regenerative Medicine, Vinmec Healthcare System, Hanoi 100000, Vietnam., Do PTX; Vinmec Center for Applied Sciences and Regenerative Medicine, Vinmec Healthcare System, Hanoi 100000, Vietnam.; Faculty of Biology, VNU University of Science, Vietnam National University, Hanoi 100000, Vietnam., Dao HH; Vinmec Center for Applied Sciences and Regenerative Medicine, Vinmec Healthcare System, Hanoi 100000, Vietnam.; Faculty of Biology, VNU University of Science, Vietnam National University, Hanoi 100000, Vietnam., Hai DX; Department of Practical and Experimental Surgery, Vietnam Military Medical University, Hanoi 12108, Vietnam., Le NT; Vinmec Center for Applied Sciences and Regenerative Medicine, Vinmec Healthcare System, Hanoi 100000, Vietnam.; Faculty of Biology, VNU University of Science, Vietnam National University, Hanoi 100000, Vietnam., Nguyen XH; Vinmec Center for Applied Sciences and Regenerative Medicine, Vinmec Healthcare System, Hanoi 100000, Vietnam.; College of Health Sciences, VinUniversity, Hanoi 100000, Vietnam., Than UTT; Vinmec Center for Applied Sciences and Regenerative Medicine, Vinmec Healthcare System, Hanoi 100000, Vietnam.
المصدر: Biomedicines [Biomedicines] 2022 Jul 27; Vol. 10 (8). Date of Electronic Publication: 2022 Jul 27.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101691304 Publication Model: Electronic Cited Medium: Print ISSN: 2227-9059 (Print) Linking ISSN: 22279059 NLM ISO Abbreviation: Biomedicines Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI AG, [2013]-
مستخلص: Umbilical cord-derived mesenchymal stem cells (UCMSCs) have been illustrated for their roles in immunological modulation and tissue regeneration through the secretome. Additionally, culture conditions can trigger the secretion of extracellular vesicles (EVs) into extracellular environments with significant bioactivities. This study aims to investigate the roles of three EV sub-populations released by UCMSCs primed with transforming growth factor β (TGFβ) and their capacity to alter dermal fibroblast functions for skin aging. Results show that three EV sub-populations, including apoptotic bodies (ABs), microvesicles (MVs), and exosomes (EXs), were separated from conditioned media. These three EVs carried growth factors, such as FGF-2, HGF, and VEGF-A, and did not express noticeable effects on fibroblast proliferation and migration. Only EX from TGFβ-stimulated UCMSCs exhibited a better capacity to promote fibroblasts migrating to close scratched wounds than EX from UCMSCs cultured in the normal condition from 24 h to 52 h. Additionally, mRNA levels of ECM genes (COL I, COL III, Elastin, HAS II, and HAS III) were detected with lower levels in fibroblasts treated with EVs from normal UCMSCs or TGFβ-stimulated UCMSCs compared to EV-depleted condition. On the contrary, the protein levels of total collagen and elastin released by fibroblasts were greater in the cell groups treated with EVs compared to EV-depleted conditions; particularly elastin associated with TGFβ-stimulated UCMSCs. These data indicate the potential roles of EVs from UCMSCs in protecting skin from aging by promoting ECM protein production.
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فهرسة مساهمة: Keywords: extracellular matrix; extracellular vesicles; fibroblast migration; fibroblast proliferation; mesenchymal stem cells; skin rejuvenation; umbilical cord-derived
تواريخ الأحداث: Date Created: 20220826 Latest Revision: 20220830
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC9405311
DOI: 10.3390/biomedicines10081810
PMID: 36009357
قاعدة البيانات: MEDLINE
الوصف
تدمد:2227-9059
DOI:10.3390/biomedicines10081810