دورية أكاديمية
أعرض في EDS

The Critical Role of Hypoxia in the Re-Differentiation of Human Articular Chondrocytes.

التفاصيل البيبلوغرافية
العنوان: The Critical Role of Hypoxia in the Re-Differentiation of Human Articular Chondrocytes.
المؤلفون: Martinez-Armenta C; Graduate Program in Experimental Biology, DCBS, Universidad Autónoma Metropolitana Iztapalapa, Mexico City 09340, Mexico.; Laboratorio de Gerociencias, Instituto Nacional de Rehabilitación 'Luis Guillermo Ibarra Ibara', Mexico City 14389, Mexico., Suarez-Ahedo C; Departamento de Reconstrucción Articular, Instituto Nacional de Rehabilitación 'Luis Guillermo Ibarra Ibara', Mexico City 14389, Mexico., Olivos-Meza A; Servicio de Medicina del Deporte y Ortopedia, Instituto Nacional de Rehabilitación 'Luis Guillermo Ibarra Ibara', Mexico City 14389, Mexico., Camacho-Rea MC; Departamento de Nutrición Animal, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Secretaria de Salud, Mexico City 14080, Mexico., Martínez-Gómez LE; Laboratorio de Gerociencias, Instituto Nacional de Rehabilitación 'Luis Guillermo Ibarra Ibara', Mexico City 14389, Mexico., Jimenez-Gutierrez GE; Laboratorio de Gerociencias, Instituto Nacional de Rehabilitación 'Luis Guillermo Ibarra Ibara', Mexico City 14389, Mexico., Martínez-Nava GA; Laboratorio de Gerociencias, Instituto Nacional de Rehabilitación 'Luis Guillermo Ibarra Ibara', Mexico City 14389, Mexico., Gomez-Quiroz LE; Laboratorio de Medicina Experimental y Carcinogénesis, Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana Iztapalapa, Mexico City 09340, Mexico., Pineda C; Dirección General, Instituto Nacional de Rehabilitación 'Luis Guillermo Ibarra Ibara', Mexico City 14389, Mexico., López-Reyes A; Laboratorio de Gerociencias, Instituto Nacional de Rehabilitación 'Luis Guillermo Ibarra Ibara', Mexico City 14389, Mexico.
المصدر: Cells [Cells] 2022 Aug 17; Vol. 11 (16). Date of Electronic Publication: 2022 Aug 17.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101600052 Publication Model: Electronic Cited Medium: Internet ISSN: 2073-4409 (Electronic) Linking ISSN: 20734409 NLM ISO Abbreviation: Cells Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI
مواضيع طبية MeSH: Cartilage, Articular*/metabolism , Chondrocytes*/metabolism, Chondrogenesis ; Humans ; Hypoxia/metabolism
مستخلص: The preservation of the chondrogenic phenotype and hypoxia-related physiological microenvironment are major challenges in the 2D culture of primary human chondrocytes. To address this problem, we develop a 3D culture system generating scaffold-free spheroids from human chondrocytes. Our results highlight the chondrogenic potential of cultured human articular chondrocytes in a 3D system combined with hypoxia independently of the cartilage source. After 14 days of culture, we developed spheroids with homogenous diameter and shape from hyaline cartilage donors. Spheroids generated in hypoxia showed a significantly increased glycosaminoglycans synthesis and up-regulated the expression of SOX9 , ACAN , COL2A1 , COMP , and SNAI1 compared to those obtained under normoxic conditions. Therefore, we conclude that spheroids developed under hypoxic conditions modulate the expression of chondrogenesis-related genes and native tissue features better than 2D cultures. Thus, this scaffold-free 3D culture system represents a novel in vitro model that can be used for cartilage biology research.
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فهرسة مساهمة: Keywords: 3D cell culture; cartilage; chondrocyte; hypoxia; re-differentiation
تواريخ الأحداث: Date Created: 20220826 Date Completed: 20220829 Latest Revision: 20221126
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC9406483
DOI: 10.3390/cells11162553
PMID: 36010629
قاعدة البيانات: MEDLINE
الوصف
تدمد:2073-4409
DOI:10.3390/cells11162553