دورية أكاديمية
Improved Methods for Bulk Cultivation and Fixation of Loxodes Ciliates for Fluorescence Microscopy.
| العنوان: | Improved Methods for Bulk Cultivation and Fixation of Loxodes Ciliates for Fluorescence Microscopy. |
|---|---|
| المؤلفون: | Seah BKB; Max Planck Institute for Biology, Max-Planck-Ring 5, 72076 Tübingen, Germany. Electronic address: kb.seah@tuebingen.mpg.de., Emmerich C; Max Planck Institute for Biology, Max-Planck-Ring 5, 72076 Tübingen, Germany., Singh A; Max Planck Institute for Biology, Max-Planck-Ring 5, 72076 Tübingen, Germany., Swart EC; Max Planck Institute for Biology, Max-Planck-Ring 5, 72076 Tübingen, Germany. Electronic address: estienne.swart@tuebingen.mpg.de. |
| المصدر: | Protist [Protist] 2022 Oct; Vol. 173 (5), pp. 125905. Date of Electronic Publication: 2022 Aug 03. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: G. Fischer Country of Publication: Germany NLM ID: 9806488 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-0941 (Electronic) Linking ISSN: 14344610 NLM ISO Abbreviation: Protist Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Jena : G. Fischer, 1998- |
| مواضيع طبية MeSH: | Methanol* , Ciliophora*, Fixatives ; Nitrates ; Zinc Sulfate ; Formaldehyde ; Microscopy, Fluorescence ; Ethanol ; Soil |
| مستخلص: | Loxodes is one of the best ecologically characterized ciliate genera with numerous intriguing physiological abilities, including gravity-sensing organelles and nitrate respiration. However, these cells have been considered challenging to cultivate in bulk, and are poorly preserved by conventional fixatives used for fluorescence microscopy. Here we describe methods to grow and harvest Loxodes cells in bulk with liquid soil extract medium, as well as a new fixative called ZFAE (zinc sulfate, formaldehyde, acetic acid, ethanol) that can fix Loxodes cells more effectively than buffered formaldehyde or methanol. We show that ZFAE is compatible with immunofluorescence and the nuclear stain DAPI. Loxodes is thus now amenable to long-term maintenance, large-scale growth, and modern cell biology investigations of monoclonal strains in laboratory conditions. (Copyright © 2022 The Authors. Published by Elsevier GmbH.. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Ciliophora; Karyorelictea; antibody; culture; cytoskeleton; fluorescence microscopy |
| المشرفين على المادة: | 0 (Fixatives) Y4S76JWI15 (Methanol) 0 (Nitrates) 7733-02-0 (Zinc Sulfate) 1HG84L3525 (Formaldehyde) 3K9958V90M (Ethanol) 0 (Soil) |
| تواريخ الأحداث: | Date Created: 20220826 Date Completed: 20221025 Latest Revision: 20221229 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.protis.2022.125905 |
| PMID: | 36027633 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1618-0941 |
|---|---|
| DOI: | 10.1016/j.protis.2022.125905 |