دورية أكاديمية
أعرض في EDS

IL1β/ TNFα/COX-2/VEGF axis responsible for effective healing potential of C-glucoside xanthone (mangiferin) based ointment in immunocompromised rats.

التفاصيل البيبلوغرافية
العنوان: IL1β/ TNFα/COX-2/VEGF axis responsible for effective healing potential of C-glucoside xanthone (mangiferin) based ointment in immunocompromised rats.
المؤلفون: Yadav VP; Department of Pharmacology and Toxicology, College of Veterinary Sciences and Animal Husbandry, UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, India., Shukla A; Department of Pharmacology and Toxicology, College of Veterinary Sciences and Animal Husbandry, UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, India. Electronic address: princu.dr@gmail.com., Choudhury S; Department of Pharmacology and Toxicology, College of Veterinary Sciences and Animal Husbandry, UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, India., Singh R; Department of Pharmacology and Toxicology, College of Veterinary Sciences and Animal Husbandry, UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, India., Anand M; Department of Veterinary Physiology, College of Veterinary Sciences and Animal Husbandry, UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, India., Prabhu SN; Department of Veterinary Pathology, College of Veterinary Sciences and Animal Husbandry, UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, India.
المصدر: Cytokine [Cytokine] 2022 Oct; Vol. 158, pp. 156012. Date of Electronic Publication: 2022 Aug 26.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 9005353 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0023 (Electronic) Linking ISSN: 10434666 NLM ISO Abbreviation: Cytokine Subsets: MEDLINE
أسماء مطبوعة: Publication: <2001- > : Oxford : Elsevier Science Ltd.
Original Publication: [Philadelphia, PA] : Saunders Scientific Publications, W.B. Saunders, [c1989-
مواضيع طبية MeSH: Xanthones*/metabolism , Xanthones*/pharmacology, Animals ; Cyclooxygenase 2/metabolism ; Glucosides/pharmacology ; Hydroxyproline/metabolism ; Hydroxyproline/pharmacology ; Interleukin-1beta/metabolism ; Ointments/metabolism ; Ointments/pharmacology ; Rats ; Skin/metabolism ; Tumor Necrosis Factor-alpha/metabolism ; Vascular Endothelial Growth Factor A/metabolism
مستخلص: Present study was conducted to undermine the wound healing potential of mangiferin vis a vis its molecular dynamics in immunocompromised excisional rat model. 120 rats were randomly and equally divided into five groups viz. group I (Healthy control), group II (Immunocompromised control), group III (Immunocompromised group treated with silver sulphadiazine), group IV (Immunocompromised group treated with 2.5 %Mangiferin) and group V (Immunocompromised group treated with 5 %Mangiferin). Immuno compromised state was achieved following intramuscular injection of Hydrocortisone @ 80 mg/kg body weight. Study was conducted for a period of 28 days. Six animals from each group were humanely sacrificed at weekly interval till day 28th of study. Planimetric analysis, biochemical studies viz. hydroxyproline assay, total protein and DNA content, antioxidative potential through LPO assay was done along with molecular studies involving expression profiling of IL1β, TNFα and COX-2 and Immunohistochemistry of angiogenic marker i.e. VEGF was performed to undermine the pharmacodynamics of mangiferin. Histopathological studies including H&E and Masson's Trichome was also performed to study histoarchitectural changes in wound healing and reparative process following application of mangiferin ointment. Study revealed significant (P ≤ 0.05) reduction in wound area measurement and significant (P ≤ 0.05) increase in wound contraction (%) following mangiferin administration in immunocompromised rats. Hydroxyproline, DNA and total protein showed significant (P ≤ 0.05) increase in skin tissues of mangiferin treated immunocompromised rats. LPO assay revealed significant (P ≤ 0.05) reduction in mangiferin treated animals. Histopathological studies of skin tissues revealed complete restoration advocating grade III of healing in 2.5% mangiferin treated group. Higher expression and strong signal intensity of VEGF was noticed in 2.5% mangiferin treatment group along with significant (P ≤ 0.05) upregulation IL1β and TNFα on day 7 in 2.5% mangiferin treatment group with significant (P ≤ 0.05) down regulation of COX-2 in mangiferin treatment group as compared to other groups i.e. group II and III. It is concluded from our study that mangiferin facilitates wound healing through improved wound closure, organized deposition of collagen deposition and granulation matrix formation.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: Angiogenic markers etc; Immunocompromised model; Mangiferin; VEGF; Wound healing
المشرفين على المادة: 0 (Glucosides)
0 (IL1B protein, rat)
0 (Interleukin-1beta)
0 (Ointments)
0 (Tumor Necrosis Factor-alpha)
0 (Vascular Endothelial Growth Factor A)
0 (Xanthones)
0 (vascular endothelial growth factor A, rat)
1M84LD0UMD (mangiferin)
EC 1.14.99.1 (Cyclooxygenase 2)
RMB44WO89X (Hydroxyproline)
تواريخ الأحداث: Date Created: 20220828 Date Completed: 20220908 Latest Revision: 20220928
رمز التحديث: 20231215
DOI: 10.1016/j.cyto.2022.156012
PMID: 36030705
قاعدة البيانات: MEDLINE
الوصف
تدمد:1096-0023
DOI:10.1016/j.cyto.2022.156012