دورية أكاديمية
Hydrogel-Embedded Poly(Lactic- co -Glycolic Acid) Microspheres for the Delivery of hMSC-Derived Exosomes to Promote Bioactive Annulus Fibrosus Repair.
| العنوان: | Hydrogel-Embedded Poly(Lactic- co -Glycolic Acid) Microspheres for the Delivery of hMSC-Derived Exosomes to Promote Bioactive Annulus Fibrosus Repair. |
|---|---|
| المؤلفون: | DiStefano TJ; Leni and Peter W. May Department of Orthopaedics, Icahn School of Medicine at Mount Sinai, New York, NY, USA., Vaso K; Department of Chemical Engineering, The Cooper Union for the Advancement of Science and Art, New York, NY, USA., Panebianco CJ; Leni and Peter W. May Department of Orthopaedics, Icahn School of Medicine at Mount Sinai, New York, NY, USA., Danias G; Leni and Peter W. May Department of Orthopaedics, Icahn School of Medicine at Mount Sinai, New York, NY, USA., Chionuma HN; Leni and Peter W. May Department of Orthopaedics, Icahn School of Medicine at Mount Sinai, New York, NY, USA., Kunnath K; Department of Chemical Engineering, New Jersey Institute of Technology, Newark, NJ, USA., Karoulias SZ; Leni and Peter W. May Department of Orthopaedics, Icahn School of Medicine at Mount Sinai, New York, NY, USA., Wang M; Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA.; Mount Sinai Center for Transformative Disease Modeling, Icahn School of Medicine at Mount Sinai, New York, NY, USA.; Icahn Institute for Data Science and Genomic Technology, Icahn School of Medicine at Mount Sinai, New York, NY, USA., Xu P; Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA.; Mount Sinai Center for Transformative Disease Modeling, Icahn School of Medicine at Mount Sinai, New York, NY, USA.; Icahn Institute for Data Science and Genomic Technology, Icahn School of Medicine at Mount Sinai, New York, NY, USA., Davé RN; Department of Chemical Engineering, New Jersey Institute of Technology, Newark, NJ, USA., Sahoo S; Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, New York, NY, USA., Weiser JR; Department of Chemical Engineering, The Cooper Union for the Advancement of Science and Art, New York, NY, USA., Iatridis JC; Orthopaedic Research Laboratories, Leni and Peter W. May Department of Orthopaedics, Icahn School of Medicine at Mount Sinai, New York, NY, USA. |
| المصدر: | Cartilage [Cartilage] 2022 Jul-Sep; Vol. 13 (3), pp. 19476035221113959. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: Sage Country of Publication: United States NLM ID: 101518378 Publication Model: Print Cited Medium: Internet ISSN: 1947-6043 (Electronic) Linking ISSN: 19476035 NLM ISO Abbreviation: Cartilage Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Los Angeles : Sage |
| مواضيع طبية MeSH: | Annulus Fibrosus* , Exosomes*/genetics , Exosomes*/metabolism , Mesenchymal Stem Cells*/metabolism, Glycols/metabolism ; Humans ; Hydrogels ; Microspheres ; Polylactic Acid-Polyglycolic Acid Copolymer/metabolism |
| مستخلص: | Objective: Intervertebral disk degeneration is a prevalent postoperative complication after discectomy, underscoring the need to develop preventative and bioactive treatment strategies that decelerate degeneration and seal annulus fibrosus (AF) defects. Human mesenchymal stem cell-derived exosomes (MSC-Exos) hold promise for cell-free bioactive repair; however, their ability to promote AF repair is poorly understood. The objective of this study was to evaluate the ability of MSC-Exos to promote endogenous AF repair processes and integrate MSC-Exos within a biomaterial delivery system. Design: We characterize biophysical and biochemical properties of normoxic (Nx) and hypoxic (Hx) preconditioned MSC-Exos from young, healthy donors and examine their effects on AF cell proliferation, migration, and gene expression. We then integrate a poly(lactic- co -glycolic acid) microsphere (PLGA µSphere) delivery platform within an interpenetrating network hydrogel to facilitate sustained MSC-Exo delivery. Results: Hx MSC-Exos led to a more robust response in AF cell proliferation and migration than Nx MSC-Exos and was selected for a downstream protection experiment. Hx MSC-Exos maintained a healthy AF cell phenotype under a TNFα challenge in vitro and attenuated catabolic responses. In all functional assays, AF cell responses were more sensitive to Hx MSC-Exos than Nx MSC-Exos. PLGA µSpheres released MSC-Exos over a clinically relevant timescale without affecting hydrogel modulus or pH upon initial embedment and µSphere degradation. Conclusions: This MSC-Exo treatment strategy may offer benefits of stem cell therapy without the need for exogenous stem cell transplantation by stimulating cell proliferation, promoting cell migration, and protecting cells from the degenerative proinflammatory microenvironment. |
| References: | Front Chem. 2018 Oct 22;6:499. (PMID: 30406081) Spine (Phila Pa 1976). 2005 Mar 1;30(5):487-96. (PMID: 15738779) J Cancer. 2017 Apr 9;8(7):1145-1152. (PMID: 28607588) J Anat. 2015 Dec;227(6):757-66. (PMID: 25913845) Acta Biochim Biophys Sin (Shanghai). 2017 Jan;49(1):1-13. (PMID: 27864283) Spine J. 2012 May;12(5):425-32. (PMID: 22627276) Biochem Biophys Res Commun. 2009 Feb 20;379(4):824-9. (PMID: 19133233) Eur Cell Mater. 2020 Nov 18;40:239-258. (PMID: 33206993) JOR Spine. 2019 Sep 07;2(3):e1061. (PMID: 31572978) Mol Cancer. 2019 Oct 27;18(1):148. (PMID: 31656200) ACS Biomater Sci Eng. 2020 Dec 14;6(12):6556-6586. (PMID: 33320618) Spine J. 2009 Mar;9(3):210-5. (PMID: 18440281) Nat Rev Rheumatol. 2019 Mar;15(3):167-179. (PMID: 30617265) Biomark Res. 2019 Apr 4;7:8. (PMID: 30992990) Free Radic Biol Med. 2019 Nov 1;143:1-15. (PMID: 31351174) J Bone Joint Surg Am. 2003 Jan;85(1):102-8. (PMID: 12533579) Cell Physiol Biochem. 2017;43(3):945-958. (PMID: 28957811) Biofabrication. 2016 Apr 25;8(2):025003. (PMID: 27108484) Spine (Phila Pa 1976). 1989 Apr;14(4):398-401. (PMID: 2718042) J Control Release. 2012 Jul 20;161(2):505-22. (PMID: 22353619) Stem Cells. 2017 Apr;35(4):851-858. (PMID: 28294454) Eur Spine J. 2008 Dec;17 Suppl 4:480-91. (PMID: 19005701) Eur Spine J. 2009 Mar;18(3):301-13. (PMID: 19104850) Spine (Phila Pa 1976). 1999 Dec 1;24(23):2475-83. (PMID: 10626310) JOR Spine. 2019 Dec 21;3(1):e1074. (PMID: 32211587) PLoS Genet. 2020 Apr 28;16(4):e1008592. (PMID: 32343702) Nucleic Acids Res. 2015 Apr 20;43(7):e47. (PMID: 25605792) Proc Natl Acad Sci U S A. 2013 Apr 30;110(18):7312-7. (PMID: 23589885) Circ Res. 2011 Sep 16;109(7):724-8. (PMID: 21835908) J Spinal Disord. 1991 Dec;4(4):428-36. (PMID: 1810565) Materials (Basel). 2019 Oct 12;12(20):. (PMID: 31614735) Spine (Phila Pa 1976). 2019 Dec 1;44(23):1613-1622. (PMID: 31730570) J Control Release. 2020 Aug 10;324:679-694. (PMID: 32534011) Int J Mol Sci. 2014 Mar 05;15(3):4007-18. (PMID: 24603539) Curr Protoc Cell Biol. 2006 Apr;Chapter 3:Unit 3.22. (PMID: 18228490) Biomaterials. 2017 Jun;129:54-67. (PMID: 28324865) Oncotarget. 2017 Oct 4;8(51):88845-88856. (PMID: 29179481) Theranostics. 2019 May 31;9(14):4084-4100. (PMID: 31281533) Acta Orthop Scand. 1969;40(1):23-42. (PMID: 4312806) Biochem Soc Trans. 2008 Dec;36(Pt 6):1224-31. (PMID: 19021530) J Control Release. 2002 Jan 17;78(1-3):199-209. (PMID: 11772461) J Anat. 2012 Dec;221(6):480-96. (PMID: 22686699) Arthritis Res Ther. 2010;12(1):R22. (PMID: 20149220) JOR Spine. 2019 Mar 01;2(1):e1043. (PMID: 31463457) Genome Biol. 2010;11(3):R25. (PMID: 20196867) J Cell Mol Med. 2018 Jan;22(1):261-276. (PMID: 28805297) Spine (Phila Pa 1976). 2016 May;41(9):743-50. (PMID: 26630431) Arthritis Res Ther. 2005;7(4):R732-45. (PMID: 15987475) Sci Rep. 2016 Nov 17;6:37360. (PMID: 27853274) Gastroenterology. 2007 Aug;133(2):647-58. (PMID: 17681183) Adv Healthc Mater. 2022 Mar;11(5):e2100596. (PMID: 34297485) Biomed Pharmacother. 2020 Nov;131:110660. (PMID: 32853910) Pediatr Res. 2019 Oct;86(4):460-470. (PMID: 30822775) J Vis Exp. 2014 Jun 01;(88):. (PMID: 24962652) Lancet. 2018 Jun 9;391(10137):2356-2367. (PMID: 29573870) Spine (Phila Pa 1976). 2008 Aug 1;33(17):1843-9. (PMID: 18670337) Exp Ther Med. 2016 Apr;11(4):1425-1432. (PMID: 27073460) Front Immunol. 2018 Nov 12;9:2575. (PMID: 30483255) J Bone Joint Surg Br. 1951 Nov;33-B(4):612-25. (PMID: 14880589) Anat Rec (Hoboken). 2015 Apr;298(4):720-6. (PMID: 25284686) Spine (Phila Pa 1976). 2018 Mar 1;43(5):E308-E315. (PMID: 29176471) Cancer Cell Int. 2021 Jan 15;21(1):55. (PMID: 33451320) Spine (Phila Pa 1976). 2008 Jul 15;33(16):1731-8. (PMID: 18628705) JOR Spine. 2018 Dec;1(4):e1036. (PMID: 30895277) J Cell Biochem. 2018 May;119(5):4150-4160. (PMID: 29243842) Eur Cell Mater. 2016 Aug 30;32:163-80. (PMID: 27572543) Nature. 2004 Sep 16;431(7006):350-5. (PMID: 15372042) Ann N Y Acad Sci. 2019 Apr;1442(1):61-78. (PMID: 30604562) Nat Rev Rheumatol. 2016 Apr;12(4):243-9. (PMID: 26729461) Int J Mol Sci. 2020 May 20;21(10):. (PMID: 32443722) AJNR Am J Neuroradiol. 2009 Mar;30(3):500-6. (PMID: 19147713) Spine (Phila Pa 1976). 1990 Aug;15(8):762-7. (PMID: 2237626) Cureus. 2019 May 7;11(5):e4613. (PMID: 31312540) J Tissue Eng Regen Med. 2012 May;6(5):348-55. (PMID: 21671407) PLoS One. 2017 Mar 28;12(3):e0174514. (PMID: 28350871) Cartilage. 2021 Oct;12(4):512-525. (PMID: 30971109) Cells. 2018 Oct 30;7(11):. (PMID: 30380806) Eur Cell Mater. 2015 Sep 21;30:104-16; discussion 116-7. (PMID: 26388614) J Biomech Eng. 2016 Feb;138(2):021007. (PMID: 26720265) J Control Release. 2015 Dec 10;219:119-128. (PMID: 26325405) J Bone Miner Metab. 2019 May;37(3):455-466. (PMID: 30187277) Biochem Biophys Res Commun. 2005 Jun 17;331(4):1185-92. (PMID: 15883001) Arthritis Res Ther. 2007;9(4):R77. (PMID: 17688691) Acta Biomater. 2020 Dec;118:215-232. (PMID: 33065285) Int J Mol Sci. 2019 Sep 21;20(19):. (PMID: 31546622) Stem Cell Res Ther. 2017 May 10;8(1):108. (PMID: 28486958) Acta Biomater. 2008 Sep;4(5):1161-71. (PMID: 18515199) Stem Cells Int. 2019 May 6;2019:8404168. (PMID: 31249601) Stem Cell Res Ther. 2019 Nov 21;10(1):340. (PMID: 31753036) Tissue Eng Part A. 2014 May;20(9-10):1379-91. (PMID: 24372278) Nat Rev Rheumatol. 2014 Sep;10(9):561-6. (PMID: 24914695) Spine (Phila Pa 1976). 2006 Mar 15;31(6):653-7. (PMID: 16540869) Acta Biomater. 2018 Sep 15;78:13-22. (PMID: 30092378) Spine (Phila Pa 1976). 2012 Apr 20;37(9):722-32. (PMID: 21897341) Acta Biomater. 2015 Oct;26:215-24. (PMID: 26116448) Nanomaterials (Basel). 2019 Feb 14;9(2):. (PMID: 30769853) Eur Cell Mater. 2013 Jan 02;25:1-21. (PMID: 23283636) Biomaterials. 2020 Nov;258:120309. (PMID: 32823020) Am J Med Sci. 2020 Dec;360(6):693-700. (PMID: 32771218) |
| معلومات مُعتمدة: | R01 AR057397 United States AR NIAMS NIH HHS; R01 AR080096 United States AR NIAMS NIH HHS; T32 GM062754 United States GM NIGMS NIH HHS |
| فهرسة مساهمة: | Keywords: drug delivery; exosomes; extracellular vesicles; hydrogels; intervertebral disk |
| المشرفين على المادة: | 0 (Glycols) 0 (Hydrogels) 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer) |
| تواريخ الأحداث: | Date Created: 20220830 Date Completed: 20220902 Latest Revision: 20220911 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC9434687 |
| DOI: | 10.1177/19476035221113959 |
| PMID: | 36040157 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1947-6043 |
|---|---|
| DOI: | 10.1177/19476035221113959 |