دورية أكاديمية
Differentiation and CRISPR-Cas9-mediated genetic engineering of human intestinal organoids.
| العنوان: | Differentiation and CRISPR-Cas9-mediated genetic engineering of human intestinal organoids. |
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| المؤلفون: | Martinez-Silgado A; Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and UMC Utrecht, 3584 CT Utrecht, the Netherlands., Yousef Yengej FA; Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and UMC Utrecht, 3584 CT Utrecht, the Netherlands; Department of Nephrology and Hypertension, University Medical Center Utrecht, 3584 CX Utrecht, the Netherlands., Puschhof J; Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and UMC Utrecht, 3584 CT Utrecht, the Netherlands; Microbiome and Cancer Division, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 280, 69120 Heidelberg, Germany., Geurts V; Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and UMC Utrecht, 3584 CT Utrecht, the Netherlands., Boot C; Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and UMC Utrecht, 3584 CT Utrecht, the Netherlands., Geurts MH; Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and UMC Utrecht, 3584 CT Utrecht, the Netherlands., Rookmaaker MB; Department of Nephrology and Hypertension, University Medical Center Utrecht, 3584 CX Utrecht, the Netherlands., Verhaar MC; Department of Nephrology and Hypertension, University Medical Center Utrecht, 3584 CX Utrecht, the Netherlands., Beumer J; Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and UMC Utrecht, 3584 CT Utrecht, the Netherlands. Electronic address: j.beumer@hubrecht.eu., Clevers H; Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and UMC Utrecht, 3584 CT Utrecht, the Netherlands; Head of Pharma, Research and Early Development (pRED) and Member of the Enlarged Executive Committee of F. Hoffmann La Roche Ltd., Basel, Switzerland. Electronic address: h.clevers@hubrecht.eu. |
| المصدر: | STAR protocols [STAR Protoc] 2022 Aug 18; Vol. 3 (3), pp. 101639. Date of Electronic Publication: 2022 Aug 18 (Print Publication: 2022). |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: eCollection Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Cambridge, MA] : Cell Press, [2020]- |
| مواضيع طبية MeSH: | CRISPR-Cas Systems*/genetics , Organoids*, Cell Differentiation/genetics ; Genetic Engineering ; Humans ; Intestines |
| مستخلص: | Intestinal organoids are three-dimensional cultures that resemble key aspects of the epithelium of origin. Here, we describe how to differentiate human small intestinal organoids by combining growth media variations and genetic engineering. We detail the differentiation of human intestinal organoids in the presence and absence of BMP agonists to recapitulate a broader scope of functional cell states found in vivo . Using transient overexpression of the transcription factor Neurogenin-3, we describe the enhancement of differentiation toward rare enteroendocrine cells. For complete details on the use and execution of this protocol, please refer to Beumer et al. (2022). Competing Interests: H.C. is head of Roche’s Pharma Research and Early Development in Basel, Switzerland since March 2022. J.B. and H.C. are inventors on several patents related to organoid technology. H.C.’s full disclosure is given at https://www.uu.nl/staff/JCClevers/. (© 2022 The Author(s).) |
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| فهرسة مساهمة: | Keywords: CRISPR; Cell differentiation; Organoids |
| تواريخ الأحداث: | Date Created: 20220831 Date Completed: 20220902 Latest Revision: 20220930 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC9420536 |
| DOI: | 10.1016/j.xpro.2022.101639 |
| PMID: | 36042877 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2666-1667 |
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| DOI: | 10.1016/j.xpro.2022.101639 |