دورية أكاديمية
[Mechanism of Panlongqi Tablets intervening in vertebral artery type of cervical spondylosis in rats through PI3K/AKT signaling pathway based on network pharmacology and experimental verification].
| العنوان: | [Mechanism of Panlongqi Tablets intervening in vertebral artery type of cervical spondylosis in rats through PI3K/AKT signaling pathway based on network pharmacology and experimental verification]. |
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| المؤلفون: | Ming RR; Institute of Chinese Materia Medica, China Academy of Chinese Medicine Sciences Beijing 100700, China., Zhang YQ; Institute of Chinese Materia Medica, China Academy of Chinese Medicine Sciences Beijing 100700, China., Xu Y; Institute of Chinese Materia Medica, China Academy of Chinese Medicine Sciences Beijing 100700, China., Xu TT; Institute of Chinese Materia Medica, China Academy of Chinese Medicine Sciences Beijing 100700, China., Fang LC; Institute of Chinese Materia Medica, China Academy of Chinese Medicine Sciences Beijing 100700, China., Wang JX; Institute of Chinese Materia Medica, China Academy of Chinese Medicine Sciences Beijing 100700, China., Wang XX; Institute of Chinese Materia Medica, China Academy of Chinese Medicine Sciences Beijing 100700, China., Hu ZX; Institute of Chinese Materia Medica, China Academy of Chinese Medicine Sciences Beijing 100700, China., Yang C; Institute of Chinese Materia Medica, China Academy of Chinese Medicine Sciences Beijing 100700, China., Jia KX; Institute of Chinese Materia Medica, China Academy of Chinese Medicine Sciences Beijing 100700, China., Wang L; Institute of Chinese Materia Medica, China Academy of Chinese Medicine Sciences Beijing 100700, China., Liu CF; Institute of Chinese Materia Medica, China Academy of Chinese Medicine Sciences Beijing 100700, China., Lin N; Institute of Chinese Materia Medica, China Academy of Chinese Medicine Sciences Beijing 100700, China. |
| المصدر: | Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica [Zhongguo Zhong Yao Za Zhi] 2022 Aug; Vol. 47 (16), pp. 4454-4461. |
| نوع المنشور: | Journal Article |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Zhongguo yao xue hui : Zhongguo Zhong yi yan jiu yuan Zhong yao yan jiu suo Country of Publication: China NLM ID: 8913656 Publication Model: Print Cited Medium: Print ISSN: 1001-5302 (Print) Linking ISSN: 10015302 NLM ISO Abbreviation: Zhongguo Zhong Yao Za Zhi Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Beijing : Zhongguo yao xue hui : Zhongguo Zhong yi yan jiu yuan Zhong yao yan jiu suo Original Publication: [Beijing] : Zhongguo yao xue hui : [Zhongguo Zhong yi yan jiu yuan Zhong yao yan jiu suo, 1989- |
| مواضيع طبية MeSH: | NF-kappa B*/metabolism , Spondylosis*/drug therapy, Animals ; Drugs, Chinese Herbal ; I-kappa B Kinase/metabolism ; I-kappa B Kinase/pharmacology ; Network Pharmacology ; Phosphatidylinositol 3-Kinases/genetics ; Phosphatidylinositol 3-Kinases/metabolism ; Protein Serine-Threonine Kinases ; Proto-Oncogene Proteins c-akt/genetics ; Proto-Oncogene Proteins c-akt/metabolism ; Rats ; Signal Transduction ; Tumor Necrosis Factor-alpha/metabolism ; Vascular Endothelial Growth Factor A/genetics ; Vertebral Artery/metabolism |
| مستخلص: | This study aimed to further explore the relevant mechanism of action by network pharmacology integrated with animal experimental verification based on previous proven effective treatment of vertebral artery type of cervical spondylosis(CSA) by Panlongqi Tablets. Bionetwork analysis was performed to establish drug-disease interaction network, and it was found that the key candidate targets of Panlongqi Tablets were enriched in multiple signaling pathways related to CSA pathological links, among which phosphatidylinositol 3-kinase(PI3 K)/serine-threonine kinase(AKT/PKB) signaling pathway was the most significant. Further, mixed modeling method was used to build the CSA rat model, and the rats were divided into normal, model, Panlongqi Tablets low-, medium-and high-dose(0.16, 0.32, 0.64 g·kg~(-1)) and Jingfukang Granules(positive drug, 1.35 g·kg~(-1)) groups. After successful modeling, the rats were administered for 8 consecutive weeks. Pathological changes of rat cervical muscle tissues were detected by hematoxylin-eosin(HE) staining, and the content of interleukin-1β(IL-1β), tumor necrosis factor-α(TNF-α), vascular endothelial cell growth factor(VEGF) and chemokine(C-C motif) ligand 2(CCL2) in rat serum and/or cervical tissues was determined by enzyme-linked immunosorbent assay(ELISA). Western blot was employed to detect the protein expression levels of chemokine(C-C motif) receptor 2(CCR2), PI3 K, AKT, phosphorylated AKT(p-AKT), I-kappa-B-kinase beta(IKK-beta/IKKβ), nuclear factor kappa B(NF-κB P65) and phosphorylated nuclear factor kappa B(NF-κB p-P65) in rat cervical tissues, and positive expression of p-NF-κB P65 in rat cervical muscle tissues was detected by immunofluorescence. The results showed that Panlongqi Tablets at different doses improved the degree of muscle fibrosis and inflammation in cervical muscle tissues of CSA rats, and reduced the content of inflammatory factors IL-1β, TNF-α, VEGF, CCL2 and CCR2 in serum and/or cervical tissues. The protein expression levels of PI3 K, p-AKT, IKKβ and p-NF-κB P65 as well as the nuclear entry of p-NF-κB P65 in cervical tissues were down-regulated. These findings suggest that Panlongqi Tablets can significantly inhibit the inflammatory response of CSA rats, and the mechanism of action may be related to the down-regulation of the activation of PI3 K/AKT signaling pathway. |
| فهرسة مساهمة: | Keywords: Panlongqi Tablets; inflammatory pathways; network pharmacology; vertebral artery type of cervical spondylosis |
| المشرفين على المادة: | 0 (Drugs, Chinese Herbal) 0 (NF-kappa B) 0 (Tumor Necrosis Factor-alpha) 0 (Vascular Endothelial Growth Factor A) 0 (panlongqi) EC 2.7.11.1 (Protein Serine-Threonine Kinases) EC 2.7.11.1 (Proto-Oncogene Proteins c-akt) EC 2.7.11.10 (I-kappa B Kinase) |
| تواريخ الأحداث: | Date Created: 20220901 Date Completed: 20220908 Latest Revision: 20220908 |
| رمز التحديث: | 20231215 |
| DOI: | 10.19540/j.cnki.cjcmm.20220302.701 |
| PMID: | 36046875 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1001-5302 |
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| DOI: | 10.19540/j.cnki.cjcmm.20220302.701 |