دورية أكاديمية
Evaluation of the biotechnological potential of peptide Cupiennin 1a and analogs.
| العنوان: | Evaluation of the biotechnological potential of peptide Cupiennin 1a and analogs. |
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| المؤلفون: | Araújo RO; Centro de Análises Proteômicas e Bioquímicas, Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, Brazil., Leite ML; Centro de Análises Proteômicas e Bioquímicas, Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, Brazil., Dutra TTB; Centro de Análises Proteômicas e Bioquímicas, Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, Brazil., Brito da Cunha N; Centro de Análises Proteômicas e Bioquímicas, Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, Brazil.; Faculdade de Agronomia e Medicina Veterinária, Universidade de Brasília - UnB, Brasília, Brazil., Rezende TMB; Centro de Análises Proteômicas e Bioquímicas, Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, Brazil.; Pós-Graduação em Ciências da Saúde, Universidade de Brasília, Brasília, Brazil., Ramada MHS; Centro de Análises Proteômicas e Bioquímicas, Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, Brazil.; Programa de Pós-Graduação em Gerontologia, Universidade Católica de Brasília, Brasília, Brazil., Dias SC; Centro de Análises Proteômicas e Bioquímicas, Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, Brazil.; Pós-Graduação ao em Biologia Animal, Campus Universitário Darcy Ribeiro, Universidade de Brasília, Brasília, Brazil. |
| المصدر: | Frontiers in microbiology [Front Microbiol] 2022 Aug 18; Vol. 13, pp. 850007. Date of Electronic Publication: 2022 Aug 18 (Print Publication: 2022). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101548977 Publication Model: eCollection Cited Medium: Print ISSN: 1664-302X (Print) Linking ISSN: 1664302X NLM ISO Abbreviation: Front Microbiol Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Research Foundation |
| مستخلص: | Antimicrobial peptides (AMPs) are components in the innate immune system of various organisms, and many AMPs can be found in poisons from animals such as spiders, scorpions, and snakes. The peptide Cupiennin-1a is present in the venom of the spider Cupiennius salei and belongs to a group of peptides called cupiennins. The peptide demonstrated high cytotoxic activity against mammalian cells; thus, aiming to solve this problem, seven analogs were designed (R1a, R1b, R2b, R3b, R6b, R8b, and R10b) based on the primary structure of the peptide Cupiennin 1a, reducing its size and substituting some amino acid residues. The antimicrobial results showed that all Cupiennin 1a analogs displayed antimicrobial activity against the tested bacterial and fungal strains. Cytotoxicity tests demonstrated a decrease in the cytotoxic effect of the analogs when compared to the peptide Cupiennin-1a. The antitumor activity against breast adenocarcinoma lines was observed for all the peptides, displaying a better effect against the MCF-7 and MDAMB-231 cell lines. The eight peptides have insecticidal potential, and the original peptide and analogs R6b, R8b, and R10b showed better efficiency even at low concentrations. The rational design of the analogs led to new molecules displaying activities against different cell types and reduced cytotoxicity toward healthy mammalian cells when compared to the original peptide, demonstrating that this was an interesting approach for the development of molecules with biotechnological potential. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Araújo, Leite, Dutra, Brito da Cunha, Rezende, Ramada and Dias.) |
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| فهرسة مساهمة: | Keywords: analogs; antimicrobial peptides; cytotoxicity; rational design; spider |
| تواريخ الأحداث: | Date Created: 20220905 Latest Revision: 20220907 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC9433906 |
| DOI: | 10.3389/fmicb.2022.850007 |
| PMID: | 36060778 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1664-302X |
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| DOI: | 10.3389/fmicb.2022.850007 |